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Cold Spring Harbor Protocols, 2009
INTRODUCTIONMany different DNA isolation methods have been employed successfully in ants. Parameters such as the size and developmental stage of the specimen (egg, larvae, or adult) and the subsequent use of the DNA will mostly determine which method should be used. Ant body sizes range from minute (1-2 mm in length) to large (30 mm), and the volume of
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INTRODUCTIONMany different DNA isolation methods have been employed successfully in ants. Parameters such as the size and developmental stage of the specimen (egg, larvae, or adult) and the subsequent use of the DNA will mostly determine which method should be used. Ant body sizes range from minute (1-2 mm in length) to large (30 mm), and the volume of
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Isolation and Quantification of DNA
Cold Spring Harbor Protocols, 2018Purifying DNA is the key to successful cloning. The cleaner the final preparation of DNA, the more efficient will be the enzymatic reactions that use the DNA as a template or a substrate. In the 1930s and 1940s, the scientific literature began to accumulate methods to release DNA from cells and to remove cellular constituents that inhibit or act as ...
Michael R, Green, Joseph, Sambrook
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Analytical Biochemistry, 1991
A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation.
T L, Kendall, D J, Byerley, R, Dean
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A rapid, economical method of DNA isolation from blood was developed that yields DNA suitable for Southern analysis and polymerase chain reactions without organic solvent extractions. Bovine DNA was prepared from peripheral leukocytes and nuclei using pronase E digestion and ethanol precipitation.
T L, Kendall, D J, Byerley, R, Dean
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Caulimovirus Isolation and DNA Extraction
1998Members of the cauhmovn-us group (1) each have a circular double-stranded DNA genome of approx 8 kbp that is encapsidated m a spherical, naked nucleocapsid of approx 50 nm diameter (Fig. 1). Caulimovnuses charactertsttcally produce subcellular mclusron bodies m infected tissues that contam most of the virions found in cells, embedded m an apparently ...
S N, Covey +3 more
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Analytical Biochemistry, 1989
Methods are described that allow DNA to be prepared from widely different yeasts (Candida utilis, Saccharomyces cerevisiae, and Schizosaccharomyces pombe). The methods are reliably reproducible, and the DNA obtained is of appropriate quality for the construction of gene libraries (upper limit of size range consistently 50-150 kbp).
W, Mann, J, Jeffery
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Methods are described that allow DNA to be prepared from widely different yeasts (Candida utilis, Saccharomyces cerevisiae, and Schizosaccharomyces pombe). The methods are reliably reproducible, and the DNA obtained is of appropriate quality for the construction of gene libraries (upper limit of size range consistently 50-150 kbp).
W, Mann, J, Jeffery
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1997
Green plants and photosynthetic organisms are unique among eukaryotes since they possess three genomes: the nuclear, the mitochondrial and the plastid. The first indication that genes were present in the cytoplasm was discovered by the inheritance of certain traits that did not follow Mendel’s rules. Even though these observations were made as early as
M. Landgren, K. Glimelius
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Green plants and photosynthetic organisms are unique among eukaryotes since they possess three genomes: the nuclear, the mitochondrial and the plastid. The first indication that genes were present in the cytoplasm was discovered by the inheritance of certain traits that did not follow Mendel’s rules. Even though these observations were made as early as
M. Landgren, K. Glimelius
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Cold Spring Harbor Protocols, 2018
This method is used to isolate yeast genomic DNA or shuttle plasmids that replicated both in Saccharomyces cerevisiae and in E. coli.
Michael R, Green, Joseph, Sambrook
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This method is used to isolate yeast genomic DNA or shuttle plasmids that replicated both in Saccharomyces cerevisiae and in E. coli.
Michael R, Green, Joseph, Sambrook
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2002
Literally hundreds of protocols for DNA preparation from various sources of tissue have been published over the last few decades. To display all of these preparations would take volumes of manual space so instead we present in this chapter several of the preparations that have been used successfully in our laboratories. We also present a few “classical”
Michele K. Nishiguchi +10 more
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Literally hundreds of protocols for DNA preparation from various sources of tissue have been published over the last few decades. To display all of these preparations would take volumes of manual space so instead we present in this chapter several of the preparations that have been used successfully in our laboratories. We also present a few “classical”
Michele K. Nishiguchi +10 more
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Isolation of Single‐Stranded DNA
Current Protocols in Molecular Biology, 2014AbstractSingle‐stranded DNA (ssDNA) is often used for DNA sequencing as well as microarray and hybridization technologies. Asymmetric PCR or exonuclease digestion followed by urea gel separation and isolation on streptavidin‐coated magnetic beads are commonly used for this purpose. These two methods may not yield large amounts of highly purified ssDNA.
Yuji, Wakimoto +2 more
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Isolation of DNA from exosomes
2020Exosomes are small extracellular vesicles released by prokaryotic and eukaryotic cells with a crucial role in cell-to-cell communication in both physiological and pathological conditions. Exosomes contain and transfer active biomolecules, including nucleic acids, proteins and lipids to target recipient cells. In the last decade, many methodologies have
Sheila, Spada +2 more
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