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Rapid isolation of eukaryotic DNA
Analytical Biochemistry, 1987Guanidine hydrochloride (GHCl) is frequently used for the isolation of RNA from eukaryotic cells. However, it appears that its use for the rapid isolation of chromosomal DNA has been little appreciated. Intact and readily digestible DNA was prepared from a range of murine tissues and cell lines which had been dispersed in GHCl and ethanol precipitated,
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Silanized polymeric nanoparticles for DNA isolation
Materials Science and Engineering: C, 2013The aim of this study is to prepare silanized polymeric nanoparticles for DNA isolation. Polymeric p(HEMA)-IMEO-PBA nanoparticles around 85.7 nm diameter, was obtained by surfactant free emulsion polymerization for DNA isolation. Synthesized nanoparticles for characterization studies were realized scanning electron microscopy (SEM), Fourier transform ...
Turkcan, Ceren +2 more
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DNA polymorphisms within SpanishTrichinella isolates
Parasitology Research, 1991A total of 13 Spanish Trichinella isolates were characterised by DNA analysis. Genomic DNA cross-hybridisation tests revealed two distinct groups that exhibited weak cross-reactions. Further diagnostic subdivision was attempted according to restriction-fragment polymorphism using a cloned T.
T, Garate +4 more
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1998
Abstract Population geneticists are relying increasingly on the study of DNA variation within and among populations. However, the feasibility of such studies is often limited by one's ability to isolate DNA from many individuals. The problem of dealing with many samples is particularly acute for the study of plants and ancient or ...
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Abstract Population geneticists are relying increasingly on the study of DNA variation within and among populations. However, the feasibility of such studies is often limited by one's ability to isolate DNA from many individuals. The problem of dealing with many samples is particularly acute for the study of plants and ancient or ...
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Recombinant DNA and gene isolation
Trends in Cardiovascular Medicine, 1993Although a formidable array of approaches fall under the broad umbrella of molecular genetics, the basic technology is elegant and conceptually simple (Watson et al. 1983, Roberts et al. 1992). It has enabled a reductionist approach to be applied to the study of genes and their sequences, as well as the transcripts that underlie organ development and ...
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2003
Blood samples for most coagulation tests are collected into 3.8% trisodium citrate in a ratio of 1 part anticoagulant to 9 parts blood. Whole-blood samples for DNA isolation can be stored at -50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats-the interface between the red cells and ...
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Blood samples for most coagulation tests are collected into 3.8% trisodium citrate in a ratio of 1 part anticoagulant to 9 parts blood. Whole-blood samples for DNA isolation can be stored at -50°C and the DNA prepared at a later stage. A more convenient method requiring less freezer space is to store buffy coats-the interface between the red cells and ...
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Isolation of Recombinant DNA Elongation Proteins
2009This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) delta, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d) replication factor C (RF-C).
van Loon, B, Ferrari, E, Hübscher, U
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1987
Within the scope of this laboratory guide it is impossible to give a full review of all the procedures used in the isolation of intact genomic DNA. However, we shall describe a general method we used which gives an excellent quality of both nuclei and DNA suitable for genomic sequencing.
Hanspeter Saluz, Jean-Pierre Jost
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Within the scope of this laboratory guide it is impossible to give a full review of all the procedures used in the isolation of intact genomic DNA. However, we shall describe a general method we used which gives an excellent quality of both nuclei and DNA suitable for genomic sequencing.
Hanspeter Saluz, Jean-Pierre Jost
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Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions
Ca-A Cancer Journal for Clinicians, 2021Michael L Cheng +2 more
exaly