Results 101 to 110 of about 2,162,763 (370)

Elongation/Compaction of Giant DNA Caused by Depletion Interaction with a Flexible Polymer [PDF]

, 2005
Structural changes in giant DNA induced by the addition of the flexible polymer PEG were examined by the method of single-DNA observation. In dilute DNA conditions, individual DNA assumes a compact state via a discrete coil-globule transition, whereas in concentrated solution, DNA molecules exhibit an extended conformation via macroscopic phase ...
arxiv   +1 more source

The 2'-5' RNA Ligase of Escherichia coli: Purification, Cloning, and Genomic Disruption [PDF]

, 1996
An RNA ligase previously detected in extracts of Escherichia coli is capable of joining Saccharomyces cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2-5 phosphodiester linkage (Greer, C., Javor, B., and Abelson, J. (1983) Cell 33,
Abelson, John N., Arn, Eric A.
core  

Nse2, a component of the Smc5-6 complex, is a SUMO ligase required for the response to DNA damage [PDF]

, 2005
The Schizosaccharomyces pombe SMC proteins Rad18 (Smc6) and Spr18 (Smc5) exist in a high-M(r) complex which also contains the non-SMC proteins Nse1, Nse2, Nse3, and Rad62.
Andrews, Emily A, Lehmann, Alan R, Palecek, Jan, Sergeant, John, Taylor, Elaine, Watts, Felicity Z   +5 more
core   +2 more sources

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS‐PAGE with low acrylamide concentration

FEBS Open Bio, EarlyView.
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang   +13 more
wiley   +1 more source

E3-targetPred: Prediction of E3-Target Proteins Using Deep Latent Space Encoding [PDF]

arXiv, 2020
Understanding E3 ligase and target substrate interactions are important for cell biology and therapeutic development. However, experimental identification of E3 target relationships is not an easy task due to the labor-intensive nature of the experiments. In this article, a sequence-based E3-target prediction model is proposed for the first time.
arxiv  

Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF [PDF]

The repair of DNA double-strand breaks by nonhomologous end-joining (NHEJ) is essential for maintenance of genomic integrity and cell viability. Central to the molecular mechanism of NHEJ is DNA ligase IV/XRCC4/XLF complex, which rejoins the DNA.
Ahnesorg, Andres, Araujo, Baker, Baumann, Ben-Omran, Blanchette, Boyer, Boyer, Buck, Carson, Costantini, Critchlow, Deshpande, Drouet, Gary Ketner, Graham, Grawunder, Grawunder, Hanakahi, Hanakahi, Hentges, Hsu, Iiizumi, Junop, Koch, Kysela, Leber, Les A. Hanakahi, Lewis, Li, Lieber, Lu, Ma, Marchetti, Mari, Mizuta, Modesti, Modesti, Mohammadi, Nicolas, Noritaka Adachi, O’Dell, O’Driscoll, Querido, Riballo, Rooney, Sarnow, Smeaton, Smith, Sriskanda, Stracker, Sumithra Jayaram, Wu, Yano, Yu   +55 more
core   +2 more sources

DNA Ligase-Mediated Translation of DNA Into Densely Functionalized Nucleic Acid Polymers

Journal of the American Chemical Society, 2012
We developed a method to translate DNA sequences into densely functionalized nucleic acids by using T4 DNA ligase to mediate the DNA-templated polymerization of 5′-phosphorylated trinucleotides containing a wide variety of appended functional groups ...
R. Hili, Jia Niu, David R. Liu
semanticscholar   +1 more source

Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater

FEBS Open Bio, EarlyView.
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui, Ariel Kushmaro, Hana Barak, Anja Poehlein, Rolf Daniel, Hans‐Jürgen Mägert   +5 more
wiley   +1 more source

DNA Ligase IV Syndrome: A Rare Cause of Growth Failure & Hypogonadism

AACE Clinical Case Reports, 2019
: Objective: DNA ligase IV syndrome is a rare genetic disorder characterized by pronounced radiosensitivity, growth failure, pancytopenia, hypogonadism, and immunodeficiency.
Jennifer J. Iyengar, MD, Shane C. Quinonez, MD, Nataliya Razumilava, MD, Barbara Soyster, MD, Yolanda R. Smith, MD, Mark T. Vander Lugt, MD, Jennifer Wyckoff, MD   +6 more
doaj  

Functional redundancy between DNA ligases I and III in DNA replication in vertebrate cells [PDF]

, 2011
In eukaryotes, the three families of ATP-dependent DNA ligases are associated with specific functions in DNA metabolism. DNA ligase I (LigI) catalyzes Okazaki-fragment ligation at the replication fork and nucleotide excision repair (NER).
Alena A. Bencsik-Theilen, Arakawa, Arakawa, Barnes, Barnes, Bentley, Bentley, Cardoso, Critchlow, Ellenberger, Emil Mladenov, Frank, Frank, Freie, Frosina, Gao, George Iliakis, Grawunder, Hiroshi Arakawa, Katja Paul, Lakshmipathy, Lee, Lindahl, Mackenney, Mackey, Minli Wang, Montecucco, Montecucco, Moser, Okano, Okano, Pascal, Perez-Jannotti, Puebla-Osorio, Sekiguchi, Simsek, Simsek, Theresa Bednar, Tomkinson, Wang, Wang, Webster, Zhang   +42 more
core   +3 more sources