Results 311 to 320 of about 337,826 (349)
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BioEssays, 1997
AbstractDNA joining enzymes play an essential role in the maintenance of genomic integrity and stability. Three mammalian genes encoding DNA ligases, LIG1, LIG3 and LIG4, have been identified. Since DNA ligase II appears to be derived from DNA ligase III by a proteolytic mechanism, the three LIG genes can account for the four biochemically distinct DNA
David S. Levin, Alan E. Tomkinson
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AbstractDNA joining enzymes play an essential role in the maintenance of genomic integrity and stability. Three mammalian genes encoding DNA ligases, LIG1, LIG3 and LIG4, have been identified. Since DNA ligase II appears to be derived from DNA ligase III by a proteolytic mechanism, the three LIG genes can account for the four biochemically distinct DNA
David S. Levin, Alan E. Tomkinson
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Current Protocols in Molecular Biology, 2011
AbstractThe DNA ligase enzyme family catalyzes the formation of a phosphodiester bond between juxtaposed 5′‐phosphate and 3′‐hydroxyl termini in duplex DNA. This activity can seal nicks in duplex DNA or join double‐stranded DNA fragments having either blunt or cohesive ends.
Gregory J S, Lohman +2 more
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AbstractThe DNA ligase enzyme family catalyzes the formation of a phosphodiester bond between juxtaposed 5′‐phosphate and 3′‐hydroxyl termini in duplex DNA. This activity can seal nicks in duplex DNA or join double‐stranded DNA fragments having either blunt or cohesive ends.
Gregory J S, Lohman +2 more
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Current Protocols in Molecular Biology, 1989
AbstractDNA ligases catalyze the formation of phosphodiester bonds between juxtaposed 5' phosphate and a 3'‐hydroxyl terminus in duplex DNA. This activity can repair single‐stranded nicks in duplex DNA and join duplex DNA restriction fragments having either blunt ends or homologous cohesive ends. Two ligases are used for nucleic acid research and their
Nicole M. Nichols +2 more
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AbstractDNA ligases catalyze the formation of phosphodiester bonds between juxtaposed 5' phosphate and a 3'‐hydroxyl terminus in duplex DNA. This activity can repair single‐stranded nicks in duplex DNA and join duplex DNA restriction fragments having either blunt ends or homologous cohesive ends. Two ligases are used for nucleic acid research and their
Nicole M. Nichols +2 more
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A DNA metalloenzyme with DNA ligase activity
Nature, 1995Single-stranded DNA can fold into well-defined sequence-dependent tertiary structures that specifically bind a variety of target molecules, raising the possibility that some folded single-stranded DNAs might exhibit catalytic activities similar to those of ribozymes and protein enzymes.
Bernard Cuenoud, Jack W. Szostak
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DNA ligases in the repair and replication of DNA
Mutation Research/DNA Repair, 2000DNA ligases are critical enzymes of DNA metabolism. The reaction they catalyse (the joining of nicked DNA) is required in DNA replication and in DNA repair pathways that require the re-synthesis of DNA. Most organisms express DNA ligases powered by ATP, but eubacteria appear to be unique in having ligases driven by NAD(+).
Dale B. Wigley +2 more
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DNA ligase-AMP adducts: Identification of yeast DNA ligase polypeptides
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1985Yeast DNA ligase is radioactively labelled in vitro by incubating a crude cell extract with [alpha-32P]ATP. The product of this reaction is the stable covalent ligase-AMP adduct, which can be characterized by its reactivity with either pyrophosphate or nicked DNA and visualized by gel electrophoresis and autoradiography.
Geoffrey R. Banks, David G. Barker
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Ligation of DNA with T4 DNA Ligase
2003Since they are involved in such important processes as DNA replication, DNA repair, and DNA recombination, DNA ligases can be found in all living cells. Two prokaryotic DNA ligases have become indispensible tools in the fields of in vitro DNA recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and
Wim Gaastra, Kirsten Hansen
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Leukemia Research, 1985
Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
Jean-Pierre Marie +9 more
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Following partial purification on sucrose gradient and/or phosphocellulose chromatography, DNA ligase was tested in peripheral white blood and bone marrow cells of nearly 100 patients with various kinds of leukemias, mainly acute leukemias. Terminal deoxynucleotidyl transferase (TdT) was tested in parallel.
Jean-Pierre Marie +9 more
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DNA Polymerases and DNA Ligases
2013DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms. Multiple enzymes have been identified from each organism, and the functional sharing of these enzymes has been investigated for both DNA ...
Sonoko Ishino, Yoshizumi Ishino
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Prokaryotic DNA Ligases Unwind Superhelical DNA
Biochemical and Biophysical Research Communications, 1996We have studied the effect on DNA topology of binding of prokaryotic DNA ligases (T4 and E. coli) to superhelical or nicked circular DNA. Performing topoisomerase I-mediated relaxation in the presence of increasing amounts of T4 ligase led to a shift in the topoisomer distribution to increasingly more negative values.
Kensal E. van Holde +3 more
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