Results 251 to 260 of about 1,734,103 (274)

Probing and Enhancing DNA Ligase Specificity via Se‐Atom‐Mutagenesis of ATP for Accurate Gene Synthesis and Detection

Angewandte Chemie - International Edition
Abstract Discriminating against base‐pair mismatches by nucleic acid‐related enzymes is essential for DNA replication, RNA replication, nucleic acid repair, transcription, and translation. However, the discrimination mechanisms at the atomic level remain poorly understood, limited by the availability of atom ...
Zhen Huang, Bei Hu
exaly   +3 more sources

Functional domains of an ATP-dependent DNA ligase 1 1Edited by A. R. Fersht

Journal of Molecular Biology, 1999
Dale Wigley, Aidan Doherty
exaly   +2 more sources

Synthesis of bisphosphonate derivatives of ATP by T4 DNA ligase, ubiquitin activating enzyme (E1) and other ligases

Biochemical Pharmacology, 2008
T4 DNA ligase and the ubiquitin activating enzyme (E1), catalyze the synthesis of ATP beta,gamma-bisphosphonate derivatives. Concerning T4 DNA ligase: (i) etidronate (pC(OH)(CH(3))p) displaced the AMP moiety of the complex E-AMP in a concentration dependent manner; (ii) the K(m) values and the rate of synthesis k(cat) (s(-1)), determined for the ...
Günther Sillero, María A., Diego, Anabel de, Pérez-Zuñiga, Francisco J., Sillero, Antonio   +3 more
openaire   +3 more sources

Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana

Plant Molecular Biology, 2001
Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA)
Y Q, Wu, B, Hohn, A, Ziemienowic
openaire   +2 more sources

Mechanistic and Kinetic Study of the ATP-Dependent DNA Ligase of Neisseria meningitidis

Biochemistry, 2003
The gene from Neisseria meningitidis serogroup A, encoding a putative, secreted ATP-dependent DNA ligase was cloned and overexpressed, and the soluble protein was purified. Mass spectrometry indicated that the homogeneous protein was adenylated as isolated, and sedimentation velocity experiments suggested that the enzyme exists as a monomer in solution.
Sophie, Magnet, John S, Blanchard
openaire   +2 more sources

Molecular cloning and characterization of Pcal_0039, an ATP-/NAD+-independent DNA ligase from hyperthermophilic archaeon Pyrobaculum calidifontis

International Journal of Biological Macromolecules, 2023
The genome sequence of hyperthermophilic archaeon Pyrobaculum calidifontis contains an open reading frame, Pcal_0039, which encodes a putative DNA ligase. Structural analysis disclosed the presence of signature sequences of ATP-dependent DNA ligases. We have heterologously expressed Pcal_0039 gene in Escherichia coli.
Qamar, Abbas, Majida Atta, Muhammad, Nisar Ahmad, Shakir, Mehwish, Aslam, Naeem, Rashid   +4 more
openaire   +2 more sources

Free-labelled fluorescent method for ATP detection assisted by T4 DNA ligase

Analytical Methods, 2017
A facile, highly selective biosensor was used for ATP quantitative analysis.
Hongyun Huang, Shui Fu, Yan Huang, Jianniao Tian, Yanchun Zhao, Shulin Zhao   +5 more
openaire   +1 more source

Functional domains of an ATP-dependent DNA ligase.

Journal of molecular biology, 1999
The crystal structure of an ATP-dependent DNA ligase from bacteriophage T7 revealed that the protein comprised two structural domains. In order to investigate the biochemical activities of these domains, we have overexpressed them separately and purified them to homogeneity. The larger N-terminal domain retains adenylation and ligase activities, though
A J, Doherty, D B, Wigley
openaire   +1 more source

Synthesis and bioevaluation of aryl hydroxamates distinguishing between NAD+ and ATP-dependent DNA ligases

MedChemComm, 2012
Identification of compounds distinguishing between NAD+ and ATP-dependent ligases is a key factor to discover new antimicrobials. Based on virtual screening experiments a series of hydroxamates distinguishing between the above two classes of enzymes are synthesized.
Vandna Kukshal, Mridul Mishra, Arya Ajay, Taran Khanam, Rahul Sharma, Divya Dube, Deepti Chopra, Rama Pati Tripathi, Ravishankar Ramachandran   +8 more
openaire   +1 more source

A new group of plant-specific ATP-dependent DNA ligases identified by protein phylogeny, hydrophobic cluster analysis and 3-dimensional modelling

Functional Plant Biology, 2005
The eukaryotic ATP-dependent DNA ligases comprise a group of orthologous proteins that have distinct roles in DNA metabolism. In contrast with the well-known DNA ligases of animal cells, the DNA ligases of plant cells are poorly described. Until now, only two DNA ligases (I and IV) genes of Arabidopsis thaliana (L.) Heynh were isolated and ...
Diego, Bonatto, Martin, Brendel, João Antonio Pêgas, Henriques   +2 more
openaire   +2 more sources