Comparative analysis of the end-joining activity of several DNA ligases. [PDF]
PLoS ONE, 2017 DNA ligases catalyze the repair of phosphate backbone breaks in DNA, acting with highest activity on breaks in one strand of duplex DNA. Some DNA ligases have also been observed to ligate two DNA fragments with short complementary overhangs or blunt ...
Robert J Bauer +6 more
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Naphthalimides Selectively Inhibit the Activity of Bacterial, Replicative DNA Ligases and Display Bactericidal Effects against Tubercle Bacilli [PDF]
Molecules, 2017 The DNA ligases, enzymes that seal breaks in the backbones of DNA, are essential for all organisms, however bacterial ligases essential for DNA replication use β-nicotinamide adenine dinucleotide as their co-factor, whereas those that are essential in ...
Malgorzata Korycka-Machala +6 more
doaj +3 more sources
The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation. [PDF]
PLoS Pathogens, 2017 Hepadnavirus covalently closed circular (ccc) DNA is the bona fide viral transcription template, which plays a pivotal role in viral infection and persistence.
Quanxin Long +11 more
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DNA ligases: progress and prospects. [PDF]
J Biol Chem, 2009 DNA ligases seal 5'-PO4 and 3'-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are essential guardians of genomic integrity, and ligase dysfunction underlies human genetic disease syndromes.
Shuman S.
europepmc +5 more sources
Thermostable DNA ligases from hyperthermophiles in biotechnology [PDF]
Frontiers in Microbiology, 2023 DNA ligase is an important enzyme ubiquitous in all three kingdoms of life that can ligate DNA strands, thus playing essential roles in DNA replication, repair and recombination in vivo.
Jingru Shi +3 more
doaj +2 more sources
Extraordinarily Stable Hairpin-Based Biosensors for Rapid Detection of DNA Ligases [PDF]
Biosensors, 2023 DNA ligases are essential enzymes involved in DNA replication and repair processes in all organisms. These enzymes seal DNA breaks by catalyzing the formation of phosphodiester bonds between juxtaposed 5′ phosphate and 3′ hydroxyl termini in double ...
Ziang Wu +6 more
doaj +2 more sources
Bacteriophage origin of some minimal ATP-dependent DNA ligases: a new structure from Burkholderia pseudomallei with striking similarity to Chlorella virus ligase [PDF]
Scientific Reports, 2021 DNA ligases, the enzymes responsible for joining breaks in the phosphodiester backbone of DNA during replication and repair, vary considerably in size and structure.
Jolyn Pan +4 more
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DNA binding with a minimal scaffold: structure-function analysis of Lig E DNA ligases. [PDF]
Nucleic Acids Res, 2018 DNA ligases join breaks in the phosphodiester backbone of DNA by catalysing the formation of bonds between opposing 5′P and 3′OH ends in an adenylation-dependent manner.
Williamson A, Grgic M, Leiros HS.
europepmc +2 more sources
Biochemical and Structural Characterisation of DNA Ligases from Bacteria and Archaea. [PDF]
Biosci Rep, 2016 DNA ligases are enzymes that seal breaks in the backbones of DNA, leading to them being essential for the survival of all organisms. DNA ligases have been studied from many different types of cells and organisms and shown to have diverse sizes and ...
Pergolizzi G, Wagner GK, Bowater RP.
europepmc +2 more sources
Molecular Microbiology, 2001 DNA ligases join breaks in the phosphodiester backbone of DNA molecules and are used in many essential reactions within the cell. All DNA ligases follow the same reaction mechanism, but they may use either ATP or NAD+ as a cofactor. All Bacteria (eubacteria) contain NAD+‐dependent DNA ligases, and the uniqueness of these enzymes to Bacteria makes them ...
Wilkinson, A, Day, J, Bowater, R
openaire +4 more sources