Results 21 to 30 of about 52,926 (249)
DNA binding with a minimal scaffold: structure–function analysis of Lig E DNA ligases [PDF]
Nucleic Acids Research, 2018 Adele Williamson +2 more
exaly +2 more sources
Joining of DNA breaks- interplay between DNA ligases and poly (ADP-ribose) polymerases [PDF]
DNA RepairAlan E Tomkinson
exaly +2 more sources
Role of Human DNA Ligases in Mediating Pharmacological Activities of Flavonoids [PDF]
International Journal of Molecular SciencesDaekyu Sun, Vijay Gokhale
exaly +2 more sources
Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
Molecules, 2021 Present in all organisms, DNA ligases catalyse the formation of a phosphodiester bond between a 3′ hydroxyl and a 5′ phosphate, a reaction that is essential for maintaining genome integrity during replication and repair.
Arqam Alomari +10 more
doaj +1 more source
Molecular Microbiology, 2001 DNA ligases join breaks in the phosphodiester backbone of DNA molecules and are used in many essential reactions within the cell. All DNA ligases follow the same reaction mechanism, but they may use either ATP or NAD+ as a cofactor. All Bacteria (eubacteria) contain NAD+‐dependent DNA ligases, and the uniqueness of these enzymes to Bacteria makes them ...
Wilkinson, A, Day, J, Bowater, R
openaire +3 more sources
Detection of ligation products of DNA linkers with 5'-OH ends by denaturing PAGE silver stain. [PDF]
PLoS ONE, 2012 To explore if DNA linkers with 5'-hydroxyl (OH) ends could be joined by commercial T4 and E. coli DNA ligase, these linkers were synthesized by using the solid-phase phosphoramidite method and joined by using commercial T4 and E. coli DNA ligases.
Feng Gao +3 more
doaj +1 more source
DNA Ligase I, the Replicative DNA Ligase [PDF]
, 2012 Multiple DNA ligation events are required to join the Okazaki fragments generated during lagging strand DNA synthesis. In eukaryotes, this is primarily carried out by members of the DNA ligase I family. The C-terminal catalytic region of these enzymes is composed of three domains: a DNA binding domain, an adenylation domain and an OB-fold domain.
Timothy R L, Howes, Alan E, Tomkinson
openaire +2 more sources
Inactivating UBE2M impacts the DNA damage response and genome integrity involving multiple cullin ligases. [PDF]
PLoS ONE, 2014 Protein neddylation is involved in a wide variety of cellular processes. Here we show that the DNA damage response is perturbed in cells inactivated with an E2 Nedd8 conjugating enzyme UBE2M, measured by RAD51 foci formation kinetics and cell based DNA ...
Scott Cukras +3 more
doaj +1 more source
Biomedicines, 2023 Ubiquitination is a post-translational modification (PTM) that is involved in proteolysis, protein–protein interaction, and signal transduction. Accumulation of mutations and genomic instability are characteristic of cancer cells, and dysfunction of the ...
Hae Ryung Chang
doaj +1 more source
Novel E3 ubiquitin ligases that regulate histone protein levels in the budding yeast Saccharomyces cerevisiae. [PDF]
PLoS ONE, 2012 Core histone proteins are essential for packaging the genomic DNA into chromatin in all eukaryotes. Since multiple genes encode these histone proteins, there is potential for generating more histones than what is required for chromatin assembly.
Rakesh Kumar Singh +3 more
doaj +1 more source