Results 121 to 130 of about 1,469,775 (287)

Maximizing eDNA detections of rare marine organisms: A case study of a delphinid species

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Environmental DNA (eDNA) is an emerging tool for surveying marine organisms. Advances in molecular assay designs and bioinformatic methods provide new opportunities for detecting rare species; however, overcoming methodological challenges of rare species detection remains an important obstacle for the widespread deployment of eDNA methods for ...
Nastassia V. Patin   +12 more
wiley   +1 more source

Metabarcoding replicate detection frequency tracks ddPCR copy number for cod and herring eDNA in ancient marine sediments

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Detecting environmental DNA (eDNA) from rare or low‐abundance aquatic species remains a major challenge, particularly when it is highly degraded, present at low concentrations, and dominated by DNA from non‐target taxa. These challenges are further amplified in sedimentary ancient DNA (sedaDNA) studies, where thousands of years can degrade ...
Elena Baños   +3 more
wiley   +1 more source

Enhanced fish detection sensitivity in aquatic systems using environmental RNA

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Environmental RNA (eRNA) can potentially augment the biological monitoring ability of the established environmental DNA (eDNA) application in conservation. Despite faster degradation, environmental RNA could offer unique advantages: it may help reduce the legacy signal compared to eDNA, provide insights into physiological status through gene ...
Ana Ramón‐Laca   +9 more
wiley   +1 more source

Prey Partitioning in a Diverse Carnivore Community: Implications for Reintroduced Fishers in Washington

open access: yesAnimal Conservation, EarlyView.
Using fecal DNA metabarcoding, we quantified dietary overlap among reintroduced fishers and sympatric coyotes, bobcats, and Pacific martens in Washington's North Cascades. Niche overlap was substantial for common prey but varied with body size, revealing fine‐scale resource partitioning that may limit fishers' access to energetically efficient prey ...
Kayla A. Shively   +7 more
wiley   +1 more source

DNA Metabarcoding: Simplifying Biodiversity

open access: yesAdvances in Zoology and Botany, 2023
Namita Goyat   +3 more
openaire   +1 more source

Comparative Efficiency of eDNA, Camera Traps and Scat Surveys to Detect a Semi‐Aquatic Mammal Across Multiple Catchments

open access: yesAnimal Conservation, EarlyView.
Monitoring semi‐aquatic mammals is essential for their conservation, but it is made difficult by their elusive lifestyle and generally low abundance. Eurasian otter (Lutra lutra) monitoring has traditionally relied on scat surveys, but eDNA and camera‐trapping are emerging as promising alternatives.
Simon Lacombe   +15 more
wiley   +1 more source

Assessing the host plants of the invasive apricot aphid, Myzus mumecola (Hemiptera: Aphididae)

open access: yesAgricultural and Forest Entomology, EarlyView.
We investigated the host plants of the invasive apricot aphid Myzus mumecola (Hemiptera: Aphididae), an emerging pest species in Europe, by combining molecular and ecological approaches. The winter host plant was confirmed as apricot by both methods.
Marta Chignola   +2 more
wiley   +1 more source

16S/18S metabarcoding versus culture for aetiological assessment in infectious keratitis: A prospective paired‐sample study

open access: yesActa Ophthalmologica, EarlyView.
Abstract Purpose Infectious keratitis (IK) is a vision‐threatening condition with diverse microbial aetiologies, yet conventional culture‐based diagnostics often have limited sensitivity. We investigated whether 16S/18S rRNA amplicon‐based next‐generation sequencing (NGS) can enhance aetiological assessment when used alongside standard microbiological ...
Sandra M. Skovdal   +6 more
wiley   +1 more source

Environmental DNA succeeds in detecting a low‐density population of endangered harlequin frogs

open access: yesConservation Science and Practice, EarlyView.
This work evaluates the use of environmental DNA (eDNA) to detect the Critically Endangered Jambato Harlequin Frog (Atelopus ignescens)—an iconic species once thought extinct and now known only from a single locality in the Ecuadorian Andes. Our study demonstrates the effectiveness of eDNA in detecting the Jambato in a low‐density amphibian population,
Mónica I. Páez‐Vacas   +14 more
wiley   +1 more source

Assessing aerial biodiversity over Keller Peninsula, King George Island, maritime Antarctica, using DNA metabarcoding

open access: yes
Antarctic ice-free areas are dominated by wind-dispersed organisms. However, which organisms arrive and circulate in Antarctica and how remain poorly understood.
Rosa, Luiz Henrique   +9 more
core   +1 more source

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