Results 131 to 140 of about 28,236 (304)

Rapid construction of insect–plant interaction networks via multiplexed long‐fragment DNA metabarcoding and NGS

open access: yesMethods in Ecology and Evolution, EarlyView.
Abstract Molecular identification of insect food webs can accurately reveal complex trophic interactions and serve as a foundation for understanding ecosystem functioning and advancing biodiversity conservation. This process typically involves DNA library construction, high‐throughput sequencing and subsequent steps, including data assembly, annotation,
Xiao‐man Zhang   +9 more
wiley   +1 more source

Prey Partitioning in a Diverse Carnivore Community: Implications for Reintroduced Fishers in Washington

open access: yesAnimal Conservation, EarlyView.
Using fecal DNA metabarcoding, we quantified dietary overlap among reintroduced fishers and sympatric coyotes, bobcats, and Pacific martens in Washington's North Cascades. Niche overlap was substantial for common prey but varied with body size, revealing fine‐scale resource partitioning that may limit fishers' access to energetically efficient prey ...
Kayla A. Shively   +7 more
wiley   +1 more source

Advancing DNA Barcoding and Metabarcoding Applications for Plants Requires Systematic Analysis of Herbarium Collections—An Australian Perspective

open access: yesFrontiers in Ecology and Evolution, 2018
Building DNA barcode databases for plants has historically been ad hoc, and often with a relatively narrow taxonomic focus. To realize the full potential of DNA barcoding for plants, and particularly its application to metabarcoding for mixed-species ...
Eleanor E. Dormontt   +15 more
doaj   +1 more source

Application of a novel molecular diagnostic method to examine the spatio‐temporal trends of Carcelia iliaca, a larval parasitoid of oak processionary moth (Thaumetopoea processionea)

open access: yesAgricultural and Forest Entomology, EarlyView.
The oak processionary moth is an invasive pest in the United Kingdom that threatens biodiversity and public health, with rising control costs highlighting the need for sustainable management alternatives Conservation biocontrol using the parasitoid fly Carcelia iliaca is promising but key biological data gaps hinder evidence‐based policy development. A
Kyle Alexander Miller   +5 more
wiley   +1 more source

Dead or Alive? Challenges in Discriminating Dietary From Host‐Associated Community via RNA and DNA Metabarcoding in a Filter Feeder

open access: yesEnvironmental DNA
Environmental DNA metabarcoding has found application in a range of research areas by simplifying the collection of high‐quality field data in a cost‐efficient way.
Isabelle Junk   +3 more
doaj   +1 more source

Assessing the host plants of the invasive apricot aphid, Myzus mumecola (Hemiptera: Aphididae)

open access: yesAgricultural and Forest Entomology, EarlyView.
We investigated the host plants of the invasive apricot aphid Myzus mumecola (Hemiptera: Aphididae), an emerging pest species in Europe, by combining molecular and ecological approaches. The winter host plant was confirmed as apricot by both methods.
Marta Chignola   +2 more
wiley   +1 more source

Refining a molecular tool kit to capture tropicalization in Mediterranean marine protected areas

open access: yesConservation Science and Practice, EarlyView.
We assess complementarity and trade‐offs between visual census and eDNA metabarcoding for monitoring fish community changes in MPAs. Abstract Tropicalization, the process by which tropical species expand their ranges poleward due to global ocean warming, is a prominent threat to Mediterranean marine ecosystems, challenging their effective management ...
Erika Frances Neave   +4 more
wiley   +1 more source

Prey DNA Detection in Lycosid Spiders Is Primarily Influenced by Time Since Feeding and Primer Choice

open access: yesEntomologia Experimentalis et Applicata, EarlyView.
Gut content analysis using DNA metabarcoding has improved understanding of predator–prey interactions involving spiders. We tested how starvation before feeding, time since feeding, spider size, and the presence of egg sacs or spiderlings affect prey DNA detection in Lycosidae.
Jeffery Marker   +3 more
wiley   +1 more source

Metabarcoding of Pollen Carried by Syrphids Reveals Novel Plant–Pollinator Interactions in a Protected Natural Area and Agricultural Sites

open access: yesEntomologia Experimentalis et Applicata, EarlyView.
Using DNA metabarcoding, this study investigates pollen transported by syrphids (Syrphidae) in the Dolomiti Bellunesi National Park and agricultural sites in Northern Italy. The analysis reveals a high diversity of visited plant taxa, including previously undocumented plant–pollinator interactions.
Serena Magagnoli   +6 more
wiley   +1 more source

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