Results 21 to 30 of about 29,684 (308)

Non‐specific amplification compromises environmental DNA metabarcoding with COI [PDF]

Methods in Ecology and Evolution, 2019
Abstract Metabarcoding extra‐organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when designing experiments, and especially so when developing community standards and legislative frameworks, is the choice of genetic marker and primer set.
Rupert A. Collins, Judith Bakker, Owen S. Wangensteen, Ana Z. Soto, Laura Corrigan, David Sims, Martin J. Genner, Stefano Mariani   +7 more
openalex   +8 more sources

Towards exhaustive community ecology via DNA metabarcoding

Molecular Ecology, 2023
AbstractExhaustive biodiversity data, covering all the taxa in an environment, would be fundamental to understand how global changes influence organisms living at different trophic levels, and to evaluate impacts on interspecific interactions. Molecular approaches such as DNA metabarcoding are boosting our ability to perform biodiversity inventories ...
Ficetola, Gentile Francesco, Taberlet, Pierre   +1 more
openaire   +4 more sources

Comparison of mechanical sorting and DNA metabarcoding for diet analysis with fresh and degraded wolf scats

Ecosphere, 2021
DNA metabarcoding has become a powerful technique for identifying the species present in a bulk environmental sample. The application of DNA metabarcoding to wildlife diet analysis is a particularly promising tool for exploring trophic interactions.
Aimee L. Massey, Gretchen H. Roffler, Tessa Vermeul, Jennifer M. Allen, Taal Levi   +4 more
doaj   +1 more source

Characterizing parasitic nematode faunas in faeces and soil using DNA metabarcoding

Parasites & Vectors, 2021
Background Gastrointestinal parasitic nematodes can impact fecundity, development, behaviour, and survival in wild vertebrate populations. Conventional monitoring of gastrointestinal parasitic nematodes in wild populations involves morphological ...
Marie Louise Davey, Kjersti Selstad Utaaker, Frode Fossøy   +2 more
doaj   +1 more source

Metabarcoding From Microbes to Mammals: Comprehensive Bioassessment on a Global Scale

Frontiers in Ecology and Evolution, 2020
Global biodiversity loss is unprecedented, and threats to existing biodiversity are growing. Given pervasive global change, a major challenge facing resource managers is a lack of scalable tools to rapidly and consistently measure Earth's biodiversity ...
Zacchaeus G. Compson, Beverly McClenaghan, Gregory A. C. Singer, Nicole A. Fahner, Mehrdad Hajibabaei, Mehrdad Hajibabaei   +5 more
doaj   +1 more source

What goes in, must come out:combining scat-based molecular diet analysis and quantification of ingested microplastics in a marine top predator [PDF]

, 2019
Context: Microplastics (plastic particles <5 mm in size) are highly available for ingestion by a wide range of organisms, either through direct consumption or indirectly, via trophic transfer, from prey to predator.
Bennett, Kimberley A., Galloway, Tamara S., Godley, Brendan J., Lindeque, Penelope K., Nelms, Sarah E., Parry, Helen E., Santillo, David   +6 more
core   +4 more sources

DNA metabarcoding data unveils invisible pollination networks [PDF]

Scientific Reports, 2017
AbstractAnimal pollination, essential for both ecological services and ecosystem functioning, is threatened by ongoing global changes. New methodologies to decipher their effects on pollinator composition to ecosystem health are urgently required. We compare the main structural parameters of pollination networks based on DNA metabarcoding data with ...
Pornon, André, Andalo, Christophe, Burrus, Monique, Escaravage, Nathalie   +3 more
openaire   +3 more sources

PIA : more accurate taxonomic assignment of metagenomic data demonstrated on sedaDNA from the North sea [PDF]

, 2020
Assigning metagenomic reads to taxa presents significant challenges. Existing approaches address some issues, but are mostly limited to metabarcoding or optimized for microbial data. We present PIA (Phylogenetic Intersection Analysis): a taxonomic binner
Allaby, Robin G., Cribdon, Becky, Gaffney, Vincent, Smith, Oliver, Ware, Roselyn   +4 more
core   +1 more source

DNA Metabarcoding of Amazonian Ichthyoplankton Swarms

PLOS ONE, 2017
Les forêts tropicales humides abritent une biodiversité extraordinaire. On pense que le bassin amazonien abrite 30 % de toutes les espèces de poissons de rivière dans le monde. Les informations sur l'écologie, la reproduction et le recrutement de la plupart des espèces font toujours défaut, ce qui entrave la gestion des pêches et le succès des ...
Marie-Eugénie Maggia, Yves Vigouroux, Jean‐François Renno, Fabrice Duponchelle, Érick Desmarais, Jesús Núñez-Rodríguez, Carmen Rosa García-Dávila, Fernando M. Carvajal‐Vallejos, Emmanuel Paradis, Jean Martin, Cédric Mariac   +10 more
openaire   +7 more sources

Sensitive and accurate DNA metabarcoding of parasitic helminth mock communities using the mitochondrial rRNA genes

Scientific Reports, 2022
Next-generation sequencing technologies have accelerated the pace of helminth DNA metabarcoding research, enabling species detection in bulk community samples.
Abigail Hui En Chan, Naowarat Saralamba, Sompob Saralamba, Jiraporn Ruangsittichai, Kittipong Chaisiri, Yanin Limpanont, Vachirapong Charoennitiwat, Urusa Thaenkham   +7 more
doaj   +1 more source