Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples [PDF]
Characterisation of freshwater benthic biodiversity using DNA metabarcoding may allow more cost-effective environmental assessments than the current morphological-based assessment methods. DNA metabarcoding methods where sorting or pre-sorting of samples
Markus Majaneva +4 more
doaj +2 more sources
Categorization of species as native or nonnative using DNA sequence signatures without a complete reference library. [PDF]
New genetic diagnostic approaches have greatly aided efforts to document global biodiversity and improve biosecurity. This is especially true for organismal groups in which species diversity has been underestimated historically due to difficulties ...
Andersen, Jeremy C +11 more
core +2 more sources
Foraging history of individual elephants using DNA metabarcoding
Individual animals should adjust diets according to food availability. We used DNA metabarcoding to construct individual-level dietary timeseries for elephants from two family groups in Kenya varying in habitat use, social position and reproductive status.
Brian A. Gill +4 more
openaire +3 more sources
Merging DNA metabarcoding and ecological network analysis to understand and build resilient terrestrial ecosystems [PDF]
Summary 1. Significant advances in both mathematical and molecular approaches in ecology offer unprecedented opportunities to describe and understand ecosystem functioning.
Evans, Darren M. +6 more
core +3 more sources
PCR replication in environmental DNA metabarcoding [PDF]
Environmental DNA (eDNA) metabarcoding is a common tool for measuring and cataloguing biodiversity, yet standard methodological approaches to generate metabarcoding data sets have yet to emerge, in part due to challenges understanding the biological and technical biases that affect eDNA profiles. Here, we explore how two experimental choices – depth of
Sabrina Shirazi +2 more
openaire +1 more source
Editorial: Bridging the gap between policy and science in assessing the health status of marine ecosystems [PDF]
Human activities,both established and emerging, increasingly affect the provision of marine ecosystem services that deliver societal and economic benefits.
Austen, Melanie +10 more
core +2 more sources
Environmental DNA metabarcoding describes biodiversity across marine gradients
Abstract In response to climate change, biodiversity patterns in the oceans are predicted to shift rapidly, thus increasing the need for efficient monitoring methods. Environmental DNA (eDNA) metabarcoding recently emerged as a potent and cost-effective candidate to answer this challenge.
Clare I M Adams +12 more
openaire +3 more sources
Environmental DNA metabarcoding has been widely touted as a powerful tool for monitoring biodiversity in marine ecosystems. However, this method still requires thorough validation and standardization before it can be widely applied for ecological ...
Kevin C. Morey +2 more
doaj +1 more source
Signal and noise in metabarcoding data.
Metabarcoding is a powerful molecular tool for simultaneously surveying hundreds to thousands of species from a single sample, underpinning microbiome and environmental DNA (eDNA) methods.
Zachary Gold +14 more
doaj +1 more source
Disentangling bias for non-destructive insect metabarcoding [PDF]
A fast and reliable method for obtaining a species-level identification is a fundamental requirement for a wide range of activities, from plant protection and invasive species management to biodiversity assessments and ecological studies.
Francesco Martoni +3 more
doaj +2 more sources

