The bacterial hitchhiker’s guide to COI: Universal primer-based COI capture probes fail to exclude bacterial DNA, but 16S capture leaves metazoa behind [PDF]
Metabarcoding and Metagenomics, 2022 Environmental DNA (eDNA) metabarcoding from water samples has, in recent years, shown great promise for biodiversity monitoring. However, universal primers targeting the cytochrome oxidase I (COI) marker gene popular in metazoan studies have displayed ...
Sanni Hintikka +2 more
doaj +3 more sources
Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding? [PDF]
PeerJ, 2018 Background High throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring.
Melissa E. Carew +2 more
doaj +2 more sources
Applications of next-generation sequencing technologies and computational tools in molecular evolution and aquatic animals conservation studies : a short review [PDF]
, 2019 Aquatic ecosystems that form major biodiversity hotspots are critically threatened due to environmental and anthropogenic stressors. We believe that, in this genomic era, computational methods can be applied to promote aquatic biodiversity conservation ...
Afiqah-Aleng, Nor +8 more
core +1 more source
Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples [PDF]
Metabarcoding and Metagenomics, 2018 Characterisation of freshwater benthic biodiversity using DNA metabarcoding may allow more cost-effective environmental assessments than the current morphological-based assessment methods. DNA metabarcoding methods where sorting or pre-sorting of samples
Markus Majaneva +4 more
doaj +2 more sources
Merging DNA metabarcoding and ecological network analysis to understand and build resilient terrestrial ecosystems [PDF]
, 2016 Summary 1. Significant advances in both mathematical and molecular approaches in ecology offer unprecedented opportunities to describe and understand ecosystem functioning.
Evans, Darren M. +6 more
core +2 more sources
Categorization of species as native or nonnative using DNA sequence signatures without a complete reference library. [PDF]
, 2019 New genetic diagnostic approaches have greatly aided efforts to document global biodiversity and improve biosecurity. This is especially true for organismal groups in which species diversity has been underestimated historically due to difficulties ...
Andersen, Jeremy C +11 more
core +2 more sources
Foraging history of individual elephants using DNA metabarcoding
Royal Society Open Science, 2023 Individual animals should adjust diets according to food availability. We used DNA metabarcoding to construct individual-level dietary timeseries for elephants from two family groups in Kenya varying in habitat use, social position and reproductive status.
Brian A. Gill +4 more
openaire +3 more sources
DNA Authentication of St John’s Wort (Hypericum perforatum L.) Commercial Products Targeting the ITS Region [PDF]
, 2019 open access articleThere is considerable potential for the use of DNA barcoding methods to authenticate raw medicinal plant materials, but their application to testing commercial products has been controversial.
Hill, Eleanor +6 more
core +1 more source
PCR replication in environmental DNA metabarcoding [PDF]
, 2020 Environmental DNA (eDNA) metabarcoding is a common tool for measuring and cataloguing biodiversity, yet standard methodological approaches to generate metabarcoding data sets have yet to emerge, in part due to challenges understanding the biological and technical biases that affect eDNA profiles. Here, we explore how two experimental choices – depth of
Sabrina Shirazi +2 more
openaire +1 more source
Environmental DNA, 2020 Environmental DNA metabarcoding has been widely touted as a powerful tool for monitoring biodiversity in marine ecosystems. However, this method still requires thorough validation and standardization before it can be widely applied for ecological ...
Kevin C. Morey +2 more
doaj +1 more source