Results 91 to 100 of about 743,722 (313)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

The Odd “RB” Phage—Identification of Arabinosylation as a New Epigenetic Modification of DNA in T4-Like Phage RB69

Viruses, 2018
In bacteriophages related to T4, hydroxymethylcytosine (hmC) is incorporated into the genomic DNA during DNA replication and is then further modified to glucosyl-hmC by phage-encoded glucosyltransferases.
Julie A. Thomas, Jared Orwenyo, Lai-Xi Wang, Lindsay W. Black   +3 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

DNA-Methylation: Master or Slave of Neural Fate Decisions?

Frontiers in Neuroscience, 2018
The pristine formation of complex organs depends on sharp temporal and spatial control of gene expression. Therefore, epigenetic mechanisms have been frequently attributed a central role in controlling cell fate determination. A prime example for this is
Stefan H. Stricker, Stefan H. Stricker, Stefan H. Stricker, Magdalena Götz, Magdalena Götz, Magdalena Götz   +5 more
doaj   +1 more source

Molecular cloning and physical mapping of murine cytomegalovirus DNA [PDF]

, 1983
Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons.
Ebeling, Angelika, Koszinowski, Ulrich H., Knust, Elisabeth, Keil, Günther M.   +3 more
core  

Helicobacter pylori-induced histone modification, associated gene expression in gastric epithelial cells, and its implication in pathogenesis. [PDF]

, 2010
Histone modifications are critical in regulating gene expression, cell cycle, cell proliferation, and development. Relatively few studies have investigated whether Helicobacter pylori, the major cause of human gastric diseases, affects histone ...
Haas, Rainer, Rainer Haas, Grant, Patrick, Kaparakis-Liaskos, Maria, Hass, Rainer, Sheila E Crowe, Patrick A. Grant, Crowe, Sheila E., Crowe Sheila E., Liechti, George, Ferrero, Richard L., Richard L. Ferrero, D. Scott Merrell, Kaparakis-Liaskos Maria, Merrell, D. Scott, Wolfgang Fischer, Fischer, Wolfgang, Liechti George, Ferrero Richard L., Ding, Song-Ze, Goldberg Joanna B., Song-Ze Ding, Ding Song-Ze, George Liechti, Patrick A Grant, Crowe, Sheila, Goldberg, Joanna, Fischer Wolfgang, Joanna B Goldberg, D Scott Merrell, Hatakeyama, Masanori, Ferrero, Richard, Merrell D. Scott, Haas Rainer, Joanna B. Goldberg, Goldberg, Joanna B., Richard L Ferrero, Sheila E. Crowe, Masanori Hatakeyama, Grant Patrick A., Hatakeyama Masanori, Grant, Patrick A., Liaskos, Maria, Maria Kaparakis-Liaskos   +43 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Analysis of 8-oxo-7, 8-dihydroGuanine formation and oxidation mediated by Fenton reaction induced DNA oxidative stress

, 2005
DNA undergoes an estimated 10,000 oxidative hits per day. Oxidative DNA damage caused by reactive oxygen species (ROS) can result in multiple base modifications, which have been implicated in mutagenesis, disease and aging.
White, Blánaid
core  

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source