Results 71 to 80 of about 299,286 (332)

Evolutionary accessibility of mutational pathways [PDF]

, 2011
Functional effects of different mutations are known to combine to the total effect in highly nontrivial ways. For the trait under evolutionary selection (`fitness'), measured values over all possible combinations of a set of mutations yield a fitness ...
de Visser, J. Arjan G. M., Franke, Jasper, Klözer, Alexander, Krug, Joachim   +3 more
core   +5 more sources

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

DNA phosphorothioation in Streptomyces lividans: mutational analysis of the dnd locus

BMC Microbiology, 2009
Background A novel DNA phosphorothioate modification (DNA sulfur modification), in which one of the non-bridging oxygen atoms in the phosphodiester bond linking DNA nucleotides is exchanged by sulphur, was found to be genetically determined by dnd or dnd-
Xu Zhongli, Li Aiying, Wang Zhijun, You Delin, He Xinyi, Wang Lianrong, Chen Shi, Liang Jingdan, Xu Tiegang, Zhou Xiufen, Deng Zixin   +10 more
doaj   +1 more source

Mutational signatures reveal mutual exclusivity of homologous recombination and mismatch repair deficiencies in colorectal and stomach tumors

Scientific Data, 2023
Decomposing somatic mutation spectra into mutational signatures and their corresponding etiologies provides a powerful approach for investigating the mechanism of DNA damage and repair.
Amir Farmanbar, Robert Kneller, Sanaz Firouzi   +2 more
doaj   +1 more source

Tumor DNA From Tumor In Situ Fluid Reveals Mutation Landscape of Minimal Residual Disease After Glioma Surgery and Risk of Early Recurrence

Frontiers in Oncology, 2021
The recurrence of glioma is a difficult problem in clinical treatment. The molecular markers of primary tumors after resection cannot fully represent the characteristics of recurrent tumors.
Jinliang Yu, Zhiyuan Sheng, Zhiyuan Sheng, Shuang Wu, Shuang Wu, Yushuai Gao, Yushuai Gao, Zhaoyue Yan, Zhaoyue Yan, Chaojie Bu, Chaojie Bu, Jianjun Gu, Jianjun Gu, Yage Bu, Yage Bu, Kaiyuan Deng, Kaiyuan Deng, Sensen Xu, Sensen Xu, Zhongcan Chen, Zhongcan Chen, Qianqian Zhang, Ajmal Zemmar, Juha Hernesniemi, Juha Hernesniemi, Meiyun Wang, Meiyun Wang, Gang Liu, Tianxiao Li, Tianxiao Li, Xingyao Bu, Xingyao Bu   +31 more
doaj   +1 more source

Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS)

Nucleic Acids Research, 1989
We have improved the "polymerase chain reaction" (PCR) to permit rapid analysis of any known mutation in genomic DNA. We demonstrate a system, ARMS (Amplification Refractory Mutation System), that allows genotyping solely by inspection of reaction mixtures after agarose gel electrophoresis. The system is simple, reliable and non-isotopic.
C R, Newton, A, Graham, L E, Heptinstall, S J, Powell, C, Summers, N, Kalsheker, J C, Smith, A F, Markham   +7 more
openaire   +3 more sources

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

What information could the main actors of liquid biopsy provide? A representative case of non-small cell lung cancer (NSCLC) [PDF]

, 2018
In non-small cell lung cancer (NSCLC), there is a consensus regarding the use of liquid biopsy, generally, to detect "druggable" mutations and, in particular, to monitor tyrosine kinase inhibitor (TKI) treatments. However, whether circulating tumor cells
Manicone, M., Mariotta, S., Pezzuto, A., Ricci, A., Rossi, Elisabetta, Scaini, M. C., Zamarchi, R.   +6 more
core   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source