Results 1 to 10 of about 6,123 (114)
Biochemical Engineering Perspective on cGAS: From Enzyme Discovery to Potential Industrial Application. [PDF]
ChembiochemThis review examines the structural and enzymatic properties of cyclic GMP‐AMP synthase, including substrate promiscuity, homologs, and engineered variants. In addition, it evaluates potential expression systems for large‐scale enzyme production and analyzes reaction and purification strategies for the synthesis of cyclic dinucleotides to connect ...
Fataeri M, Rosenthal K.
europepmc +2 more sources
The arms race between bacteria CBASS and bacteriophages
Frontiers in Immunology, 2023 The Bacterial Cyclic oligonucleotide-Based Anti-phage Signaling System (CBASS) is an innate immune system that induces cell suicide to defend against phage infections.
Lan Wang +5 more
doaj +1 more source
Functional Dissection of the DNA Interface of the Nucleotidyltransferase Domain of Chlorella Virus DNA Ligase [PDF]
Journal of Biological Chemistry, 2011 Chlorella virus DNA ligase (ChVLig) has pluripotent biological activity and an intrinsic nick-sensing function. ChVLig consists of three structural modules that envelop nicked DNA as a C-shaped protein clamp: a nucleotidyltransferase (NTase) domain and an OB domain (these two are common to all DNA ligases) as well as a distinctive β-hairpin latch ...
Poulami, Samai, Stewart, Shuman
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Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli DNA Ligase (LigA) [PDF]
Journal of Biological Chemistry, 2009 NAD(+)-dependent DNA ligases (LigA) are ubiquitous in bacteria, where they are essential for growth and present attractive targets for antimicrobial drug discovery. LigA has a distinctive modular structure in which a nucleotidyltransferase catalytic domain is flanked by an upstream NMN-binding module and by downstream OB-fold, zinc finger, helix ...
Li Kai, Wang, Hui, Zhu, Stewart, Shuman
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Kinetic Analysis of DNA Strand Joining by Chlorella Virus DNA Ligase and the Role of Nucleotidyltransferase Motif VI in Ligase Adenylylation [PDF]
Journal of Biological Chemistry, 2012 Chlorella virus DNA ligase (ChVLig) is an instructive model for mechanistic studies of the ATP-dependent DNA ligase family. ChVLig seals 3'-OH and 5'-PO(4) termini via three chemical steps: 1) ligase attacks the ATP α phosphorus to release PP(i) and form a covalent ligase-adenylate intermediate; 2) AMP is transferred to the nick 5'-phosphate to form ...
Poulami, Samai, Stewart, Shuman
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Frontiers in Microbiology, 2016 Here we report the complete genome sequence of the chemoorganotrophic, extremely thermophilic bacterium, Dictyoglomus turgidum, which is a Gram negative, strictly anaerobic bacterium. D. turgidum and D.
Phillip Brumm +6 more
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Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli NAD+-dependent DNA Ligase (LigA) [PDF]
Journal of Biological Chemistry, 2005 NAD+-dependent DNA ligase (LigA) is essential for bacterial growth and a potential target for antimicrobial drug discovery. Here we queried the role of 14 conserved amino acids of Escherichia coli LigA by alanine scanning and thereby identified five new residues within the nucleotidyltransferase domain as being essential for LigA function in vitro and ...
Hui, Zhu, Stewart, Shuman
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Role of nucleotidyltransferase motifs I, III and IV in the catalysis of phosphodiester bond formation by Chlorella virus DNA ligase [PDF]
Nucleic Acids Research, 2002 ATP-dependent DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at DNA nicks by means of a series of three nucleotidyl transfer steps. Here we have analyzed by site-directed mutagenesis the roles of conserved amino acids of Chlorella virus DNA ligase during the third step of the ligation pathway, which entails reaction of the 3 ...
Verl, Sriskanda, Stewart, Shuman
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mBio, 2015 The aminoglycosides are highly effective broad-spectrum antimicrobial agents. However, their efficacy is diminished due to enzyme-mediated covalent modification, which reduces affinity of the drug for the target ribosome.
Georgina Cox +3 more
doaj +1 more source
Frontiers in Microbiology, 2019 Metagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR).
Pablo Rovira +13 more
doaj +1 more source