Nucleic Acids Research, 1999 A detailed analysis of the polbeta superfamily of nucleotidyltransferases was performed using computer methods for iterative database search, multiple alignment, motif analysis and structural modeling. Three previously uncharacterized families of predicted nucleotidyltransferases are described. One of these new families includes small proteins found in
L, Aravind, E V, Koonin
openaire +5 more sources
Biochemical Engineering Perspective on cGAS: From Enzyme Discovery to Potential Industrial Application. [PDF]
ChembiochemThis review examines the structural and enzymatic properties of cyclic GMP‐AMP synthase, including substrate promiscuity, homologs, and engineered variants. In addition, it evaluates potential expression systems for large‐scale enzyme production and analyzes reaction and purification strategies for the synthesis of cyclic dinucleotides to connect ...
Fataeri M, Rosenthal K.
europepmc +2 more sources
Role of nucleotidyltransferase motifs I, III and IV in the catalysis of phosphodiester bond formation by Chlorella virus DNA ligase [PDF]
Nucleic Acids Research, 2002 ATP-dependent DNA ligases catalyze the sealing of 5'-phosphate and 3'-hydroxyl termini at DNA nicks by means of a series of three nucleotidyl transfer steps. Here we have analyzed by site-directed mutagenesis the roles of conserved amino acids of Chlorella virus DNA ligase during the third step of the ligation pathway, which entails reaction of the 3 ...
Verl, Sriskanda, Stewart, Shuman
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MINTmap: fast and exhaustive profiling of nuclear and mitochondrial tRNA fragments from short RNA-seq data. [PDF]
, 2017 Transfer RNA fragments (tRFs) are an established class of constitutive regulatory molecules that arise from precursor and mature tRNAs. RNA deep sequencing (RNA-seq) has greatly facilitated the study of tRFs.
Loher, Phillipe +2 more
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Frontiers in Microbiology, 2019 Metagenomic investigations have the potential to provide unprecedented insights into microbial ecologies, such as those relating to antimicrobial resistance (AMR).
Pablo Rovira +13 more
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Characterization of wild and captive baboon gut microbiota and their antibiotic resistomes [PDF]
, 2018 Antibiotic exposure results in acute and persistent shifts in the composition and function of microbial communities associated with vertebrate hosts.
Boolchandani, Manish +9 more
core +2 more sources
Biology Direct, 2012 Background Proteinaceous toxins are observed across all levels of inter-organismal and intra-genomic conflicts. These include recently discovered prokaryotic polymorphic toxin systems implicated in intra-specific conflicts.
Zhang Dapeng +4 more
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Mechanism of Mg 2+ -accompanied product release in sugar nucleotidyltransferases [PDF]
, 2018 The nucleotidyl transfer reaction, catalyzed by sugar nucleotidyltransferases (SNTs), is assisted by two active site Mg 2+ ions. While studying this reaction using X-ray crystallography, we captured snapshots of the pyrophosphate (product) as it exits ...
Awasthi, Shalini +6 more
core +1 more source
NF45 dimerizes with NF90, Zfr and SPNR via a conserved domain that has a nucleotidyltransferase fold [PDF]
, 2012 Nuclear factors NF90 and NF45 form a complex involved in a variety of cellular processes and are thought to affect gene expression both at the transcriptional and translational level.
Adams +47 more
core +1 more source
Biophysical and enzymatic properties of aminoglycoside adenylyltransferase AadA6 from Pseudomonas aeruginosa [PDF]
, 2015 The gene coding for the aminoglycoside adenylyltransferase (aadA6) from a clinical isolate of Pseudomonas aeruginosa was cloned and expressed in Escherichia coli strain BL21(DE3)pLysS.
Akdemir, G. +9 more
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