Results 1 to 10 of about 598,814 (220)

DNA wrapping in transcription initiation by RNA polymerase II [PDF]

Biochemistry and Cell Biology, 1999
The DNA wrapping model of transcription stipulates that DNA bending and wrapping around RNA polymerase causes an unwinding of the DNA helix at the enzyme catalytic center that stimulates strand separation prior to initiation and during transcript elongation.
Benoit Coulombe
  +6 more sources

Differential impact of divalent metals on native elongating transcript sequencing (NET-seq) protocols for RNA polymerases I and II. [PDF]

PLoS ONE
Throughout all domains of life, RNA polymerases (Pols) synthesize RNA from DNA templates, a process called transcription. During transcription, Pols require divalent metal cations for nucleotide addition and cleavage of the nascent RNA after ...
Abigail K Huffines, David A Schneider
doaj   +2 more sources

Mechanism of DNA polymerase II-mediated frameshift mutagenesis [PDF]

Proceedings of the National Academy of Sciences, 2001
Escherichia coli possesses three SOS-inducible DNA polymerases (Pol II, IV, and V) that were recently found to participate in translesion synthesis and mutagenesis. Involvement of these polymerases appears to depend on the nature of the lesion and its local sequence context, as illustrated by the bypass of a single ...
Olivier J. Bécherel, Robert P. Fuchs
openalex   +6 more sources

Maintenance of long-range DNA interactions after inhibition of ongoing RNA polymerase II transcription. [PDF]

PLoS ONE, 2008
A relationship exists between nuclear architecture and gene activity and it has been proposed that the activity of ongoing RNA polymerase II transcription determines genome organization in the mammalian cell nucleus.
Robert-Jan Palstra, Marieke Simonis, Petra Klous, Emilie Brasset, Bart Eijkelkamp, Wouter de Laat   +5 more
doaj   +3 more sources

DNA-directed termination of RNA polymerase II transcription [PDF]

Molecular Cell, 2023
RNA polymerase II (RNAPII) transcription involves initiation from a promoter, transcriptional elongation through the gene, and termination in the terminator region. In bacteria, terminators often contain specific DNA elements provoking polymerase dissociation, but RNAPII transcription termination is thought to be driven entirely by protein co-factors ...
Zhong Han, George A. Moore, Richard Mitter, David Lopez Martinez, Li Wan, A. Barbara Dirac Svejstrup, David S. Rueda, Jesper Q. Svejstrup   +7 more
openaire   +3 more sources

Structure of an Intranucleosomal DNA Loop That Senses DNA Damage during Transcription

Cells, 2022
Transcription through chromatin by RNA polymerase II (Pol II) is accompanied by the formation of small intranucleosomal DNA loops containing the enzyme (i-loops) that are involved in survival of core histones on the DNA and arrest of Pol II during the ...
Nadezhda S. Gerasimova, Olesya I. Volokh, Nikolay A. Pestov, Grigory A. Armeev, Mikhail P. Kirpichnikov, Alexey K. Shaytan, Olga S. Sokolova, Vasily M. Studitsky   +7 more
doaj   +1 more source

Transcription shapes DNA replication initiation to preserve genome integrity

Genome Biology, 2021
Background Early DNA replication occurs within actively transcribed chromatin compartments in mammalian cells, raising the immediate question of how early DNA replication coordinates with transcription to avoid collisions and DNA damage.
Yang Liu, Chen Ai, Tingting Gan, Jinchun Wu, Yongpeng Jiang, Xuhao Liu, Rusen Lu, Ning Gao, Qing Li, Xiong Ji, Jiazhi Hu   +10 more
doaj   +1 more source

Acceleration of DNA Replication of Klenow Fragment by Small Resisting Force [PDF]

Chin. Phys. Lett. 2021, 38 (11): 118701, 2021
DNA polymerases are an essential class of enzymes or molecular motors that catalyze processive DNA syntheses during DNA replications. A critical issue for DNA polymerases is their molecular mechanism of processive DNA replication. We have previously proposed a model for chemomechanical coupling of DNA polymerases, based on which the predicted results ...
arxiv   +1 more source

DNA photodamage recognition by RNA polymerase II [PDF]

FEBS Letters, 2007
During gene transcription, RNA polymerase (Pol) II encounters obstacles, including lesions in the DNA template. Here, we review a recent structure–function analysis of Pol II transcribing DNA with a bulky photo‐lesion in the template strand. The study provided the molecular basis for recognition of a damaged DNA by Pol II, which is the first step in ...
Brückner, F., Cramer, P.
openaire   +3 more sources

DNA damage-induced transcription stress triggers the genome-wide degradation of promoter-bound Pol II

Nature Communications, 2022
DNA damage inhibits elongating RNA polymerase II, but also initiates genome-wide transcriptional responses. Here the authors reveal that particularly promoter-bound Pol II is degraded upon DNA damage in a GSK3 signaling-mediated response.
Barbara Steurer, Roel C. Janssens, Marit E. Geijer, Fernando Aprile-Garcia, Bart Geverts, Arjan F. Theil, Barbara Hummel, Martin E. van Royen, Bastiaan Evers, René Bernards, Adriaan B. Houtsmuller, Ritwick Sawarkar, Jurgen Marteijn   +12 more
doaj   +1 more source