Results 1 to 10 of about 812,933 (320)
Mechanism of DNA polymerase II-mediated frameshift mutagenesis [PDF]
Proceedings of the National Academy of Sciences, 2001 Escherichia coli possesses three SOS-inducible DNA polymerases (Pol II, IV, and V) that were recently found to participate in translesion synthesis and mutagenesis. Involvement of these polymerases appears to depend on the nature of the lesion and its local sequence context, as illustrated by the bypass of a single ...
Olivier J. Bécherel, Robert P. Fuchs
semanticscholar +7 more sources
Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase II [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1972 A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine and assay of polymerase activity in extracts of survivors.
Judith L. Campbell +2 more
openalex +2 more sources
Mechanism of Translesion DNA Synthesis by DNA Polymerase II [PDF]
Journal of Biological Chemistry, 1996 Bypass synthesis by DNA polymerase II was studied using a synthetic 40-nucleotide-long gapped duplex DNA containing a site-specific abasic site analog, as a model system for mutagenesis associated with DNA lesions.
T. Paz-Elizur +4 more
semanticscholar +4 more sources
Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. [PDF]
Proceedings of the National Academy of Sciences, 1971 The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+),
T. Kornberg, M. Gefter
semanticscholar +4 more sources
A phenotype for enigmatic DNA polymerase II: A pivotal role for pol II in replication restart in UV-irradiated Escherichia coli [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1999 DNA synthesis in Escherichia coli is inhibited transiently after UV irradiation. Induced replisome reactivation or "replication restart" occurs shortly thereafter, allowing cells to complete replication of damaged genomes.
Savithri Rangarajan +2 more
openalex +2 more sources
DNA wrapping in transcription initiation by RNA polymerase II [PDF]
Biochemistry and Cell Biology, 1999 The DNA wrapping model of transcription stipulates that DNA bending and wrapping around RNA polymerase causes an unwinding of the DNA helix at the enzyme catalytic center that stimulates strand separation prior to initiation and during transcript elongation.
Benoit Coulombe
+6 more sources
Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins.
Journal of Biological Chemistry, 1992 An interesting property of the Escherichia coli DNA polymerase II is the stimulation in DNA synthesis mediated by the DNA polymerase III accessory proteins beta,gamma complex. In this paper we have studied the basis for the stimulation in pol II activity and have concluded that these accessory proteins stimulate pol II activity by increasing the ...
Cynthia A. BonnerS +8 more
semanticscholar +4 more sources
Purification of DNA polymerase II, a distinct DNA polymerase, from Saccharomyces cerevisiae.
Journal of Biological Chemistry, 1989 Yeast DNA polymerases I and III have been well characterized physically, biochemically, genetically and immunologically. DNA polymerase II is present in very small amounts, and only partially purified preparations have been available for characterization, making comparison with DNA polymerases I and III difficult.
M. Budd, K. Sitney, J. Campbell
semanticscholar +3 more sources
Nature Communications, 2022 Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that modulates target gene expression in response to changes in oxygen availability. Here the authors show that HIF-1 forms a complex with TRIM28 and DNA-dependent protein kinase (DNA-PK) that ...
Yongkang Yang +5 more
doaj +2 more sources
Escherichia coli DNA polymerase II is homologous to α-like DNA polymerases
Molecular and General Genetics MGG, 1991 The Escherichia coli polB gene encodes DNA polymerase II and is regulated by the SOS system. We sequenced a 4081 nucleotide segment of the E. coli chromosome that contains the polB gene and its flanking regions. DNA polymerase II, as deduced from the DNA sequence, consists of 782 amino acids, has a molecular weight of 89,917, and is structurally ...
H. Iwasaki +4 more
semanticscholar +4 more sources