Results 11 to 20 of about 900,531 (345)
Mechanism of Translesion DNA Synthesis by DNA Polymerase II [PDF]
Journal of Biological Chemistry, 1996 Bypass synthesis by DNA polymerase II was studied using a synthetic 40-nucleotide-long gapped duplex DNA containing a site-specific abasic site analog, as a model system for mutagenesis associated with DNA lesions. Bypass synthesis involved a rapid polymerization step terminating opposite the nucleotide preceding the lesion, followed by a slow bypass ...
Zvi Livneh +4 more
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Purification and DNA Synthesis in Cell-Free Extracts: Properties of DNA Polymerase II [PDF]
Proceedings of the National Academy of Sciences, 1971 The major DNA-synthesizing enzyme present in Pol A 1 - Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg ++ , NH 4
Thomas B. Kornberg, Malcolm L. Gefter
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Processive DNA synthesis by DNA polymerase II mediated by DNA polymerase III accessory proteins.
Journal of Biological Chemistry, 1992 An interesting property of the Escherichia coli DNA polymerase II is the stimulation in DNA synthesis mediated by the DNA polymerase III accessory proteins beta,gamma complex. In this paper we have studied the basis for the stimulation in pol II activity and have concluded that these accessory proteins stimulate pol II activity by increasing the ...
Mike O'Donnell +7 more
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Purification of DNA Polymerase II, a Distinct DNA Polymerase, from Saccharomyces cerevisiae
Journal of Biological Chemistry, 1989 Yeast DNA polymerases I and III have been well characterized physically, biochemically, genetically and immunologically. DNA polymerase II is present in very small amounts, and only partially purified preparations have been available for characterization, making comparison with DNA polymerases I and III difficult.
Martin E. Budd +2 more
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Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase II [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1972 A mutant of Escherichia coli deficient in DNA polymerase II has been isolated from E. coli polA1 by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine and assay of polymerase activity in extracts of survivors.
Judith L. Campbell +2 more
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Genome Biology, 2018 Background Alternative DNA secondary structures can arise from single-stranded DNA when duplex DNA is unwound during DNA processes such as transcription, resulting in the regulation or perturbation of these processes. We identify sites of high propensity
Karol Szlachta +5 more
doaj +2 more sources
The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II
Nature Communications, 2020 The response to DNA damage-stalled RNA polymerase II leads to the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. Here, the authors reveal the complex assembly mechanism of the TCR complex in human cells.
Yana van der Weegen +11 more
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A phenotype for enigmatic DNA polymerase II: A pivotal role for pol II in replication restart in UV-irradiated Escherichia coli [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 1999 DNA synthesis in Escherichia coli is inhibited transiently after UV irradiation. Induced replisome reactivation or "replication restart" occurs shortly thereafter, allowing cells to complete replication of damaged genomes.
Savithri Rangarajan +2 more
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DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America, 1990 The structural gene for DNA polymerase II was cloned by using a synthetic inosine-containing oligonucleotide probe corresponding to 11 amino acids, which were determined by sequencing the amino terminus of the purified protein.
C A Bonner +3 more
openalex +2 more sources
Structure of the p53/RNA polymerase II assembly
Communications Biology, 2021 Liou et al. report a 4.6 Å resolution structure of the human p53/ RNA polymerase II assembly, using single particle cryoelectron microscopy. This study suggests that p53’s functional domains regulate the DNA binding activity of RNA polymerase II ...
Shu-Hao Liou +4 more
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