Results 21 to 30 of about 1,237,824 (318)
Purification of DNA polymerase II, a distinct DNA polymerase, from Saccharomyces cerevisiae.
Journal of Biological Chemistry, 1989 Yeast DNA polymerases I and III have been well characterized physically, biochemically, genetically and immunologically. DNA polymerase II is present in very small amounts, and only partially purified preparations have been available for characterization, making comparison with DNA polymerases I and III difficult.
M. Budd, K. Sitney, J. Campbell
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Mechanism of DNA polymerase II-mediated frameshift mutagenesis [PDF]
Proceedings of the National Academy of Sciences, 2001 Escherichia coli possesses three SOS-inducible DNA polymerases (Pol II, IV, and V) that were recently found to participate in translesion synthesis and mutagenesis. Involvement of these polymerases appears to depend on the nature of the lesion and its local sequence context, as illustrated by the bypass of a single ...
O. Becherel, R. Fuchs
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Escherichia coli DNA polymerase II is homologous to α-like DNA polymerases
Molecular and General Genetics MGG, 1991 The Escherichia coli polB gene encodes DNA polymerase II and is regulated by the SOS system. We sequenced a 4081 nucleotide segment of the E. coli chromosome that contains the polB gene and its flanking regions. DNA polymerase II, as deduced from the DNA sequence, consists of 782 amino acids, has a molecular weight of 89,917, and is structurally ...
H. Iwasaki +4 more
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DNA-directed termination of RNA polymerase II transcription [PDF]
Molecular Cell, 2023 RNA polymerase II (RNAPII) transcription involves initiation from a promoter, transcriptional elongation through the gene, and termination in the terminator region. In bacteria, terminators often contain specific DNA elements provoking polymerase dissociation, but RNAPII transcription termination is thought to be driven entirely by protein co-factors ...
Zhong Han +7 more
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Structure of an Intranucleosomal DNA Loop That Senses DNA Damage during Transcription
Cells, 2022 Transcription through chromatin by RNA polymerase II (Pol II) is accompanied by the formation of small intranucleosomal DNA loops containing the enzyme (i-loops) that are involved in survival of core histones on the DNA and arrest of Pol II during the ...
Nadezhda S. Gerasimova +7 more
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Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. [PDF]
Proceedings of the National Academy of Sciences, 1971 The major DNA-synthesizing enzyme present in Pol A 1 - Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg ++ , NH 4
T. Kornberg, M. Gefter
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Transcription shapes DNA replication initiation to preserve genome integrity
Genome Biology, 2021 Background Early DNA replication occurs within actively transcribed chromatin compartments in mammalian cells, raising the immediate question of how early DNA replication coordinates with transcription to avoid collisions and DNA damage.
Yang Liu +10 more
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Nature Communications, 2022 DNA damage inhibits elongating RNA polymerase II, but also initiates genome-wide transcriptional responses. Here the authors reveal that particularly promoter-bound Pol II is degraded upon DNA damage in a GSK3 signaling-mediated response.
Barbara Steurer +12 more
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DNA photodamage recognition by RNA polymerase II [PDF]
FEBS Letters, 2007 During gene transcription, RNA polymerase (Pol) II encounters obstacles, including lesions in the DNA template. Here, we review a recent structure–function analysis of Pol II transcribing DNA with a bulky photo‐lesion in the template strand. The study provided the molecular basis for recognition of a damaged DNA by Pol II, which is the first step in ...
Brückner, F., Cramer, P.
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SnapShot: DNA Polymerases II Mammals
Cell, 2010 DNA polymerases ensure the faithful duplication of genetic information inside the nuclease and mitochondria of eukaryotic cells and the nucleoid of prokaryotic cells. These remarkable enzymes synthesize polynucleotide chains based on the complementarity of an incoming nucleotide to an existing DNA template. DNA polymerases are grouped into six families
Foti, James J., Walker, Graham C.
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