Results 321 to 330 of about 900,531 (345)
Some of the next articles are maybe not open access.
Nature, 1970
A membrane associated DNA polymerizing enzyme has been solubilized, partially isolated and characterized.
R. Knippers
openaire +4 more sources
A membrane associated DNA polymerizing enzyme has been solubilized, partially isolated and characterized.
R. Knippers
openaire +4 more sources
The EMBO Journal, 1992 Two temperature-sensitive DNA polymerase II mutants (pol2-9 and pol2-18) of the yeast Saccharomyces cerevisiae were isolated by the plasmid shuffling method. DNA polymerase II activity partially purified from both mutants was thermolabile, while DNA polymerase I and III activities remained thermotolerant.
L H Johnston +5 more
openaire +4 more sources
Crystallization of DNA Polymerase II from Escherichia coli
Journal of Molecular Biology, 1994DNA polymerase II of Escherichia coli, an alpha-like or group B polymerase, has been crystallized. The crystals are orthorhombic, space group P2(1)2(1)2, with cell dimensions a = 94.4 A, b = 118.2 A, c = 84.2 A and diffract to at least 3.0 A resolution. This is the first example of a group B polymerase to be crystallized.
Myron F. Goodman +4 more
openaire +4 more sources
Role of DNA Polymerase II in Repair Replication in Escherichia coli
Nature New Biology, 1973Escherichia coli maintains at least three distinct enzymes capable of synthesizing DNA. Strains deficient in DNA polymerase I activity in vitro1 show increased sensitivity to ultraviolet radiation, suggesting that DNA polymerase I is involved in the repair of damaged DNA2,3.
Philip C. Hanawalt +2 more
openaire +4 more sources
Studies on the mechanism of enzymatic DNA elongation by Escherichia coli DNA polymerase II
Journal of Molecular Biology, 1976Abstract The mechanism of enzymatic elongation by Escherichia coli DNA polymerase II of a DNA primer, which is annealed to a unique position on the bacteriophage fd viral DNA, has been studied. The enzyme is found to dissociate from the substrate at specific positions on the genome which act as “barriers” to further primer extension.
Malcolm L. Gefter, Linda A. Sherman
openaire +4 more sources
Inhibition of E. coli DNA Polymerase II by Ara-CTP
Nature New Biology, 1971THE potent antileukaemic agent, 1-β-D-arabinofuranosylcytosine (ara-C), specifically inhibits DNA synthesis in bacterial and animal cells1,2. Although the exact mechanism of inhibition has been in doubt, it seems likely that it occurs at the DNA polymerization reaction itself2–5. We describe here the effects of ara-CTP, which is the most prominent form
G. V. Rama Reddy +2 more
openaire +4 more sources
Molecular Modeling of RNA Polymerase II Mutations onto DNA Polymerase I
Journal of Molecular Biology, 1994Genetic and molecular analysis in Drosophila melanogaster identifies eight suppressor mutations in the second largest subunit of RNA polymerase II. The suppressor mutations fall into two classes: five are strong, result from the same serine to cysteine amino acid residue substitution and rescue one conditional lethal allele in the largest subunit of ...
Wan Joon Kim +2 more
openaire +4 more sources
DNA Polymerase II, the Epsilon Polymerase of Saccharomyces cerevisiae
1993Publisher Summary This chapter discusses the data on DNA polymerase II in the context of current knowledge of eukaryotic DNA polymerases, DNA replication and its fidelity, and DNA repair. DNA polymerase II has been proffered as the “repair polymerase.” Contrary to the implicit assumption, DNA repair is not a single process but an array of different ...
Alan Morrison, Akio Sugino
openaire +3 more sources
DNA Melting on Yeast RNA Polymerase II Promoters
Science, 1993Transcription-dependent DNA melting on the yeast GAL1 and GAL10 promoters was found to be more closely correlated with the TATA box than the transcription start site. On both these genes, melting begins about 20 base pairs downstream of the TATA box. Physical and genetic analyses suggest that
Charles Giardina, John T. Lis
openaire +3 more sources