Results 331 to 340 of about 900,531 (345)

SOS-inducible DNA polymerase II of E coli is homologous to replicative DNA polymerase of eukaryotes

Biochimie, 1991
The polB gene of Escherichia coli encodes DNA polymerase II whose role in vivo is not defined. The polB gene has been cloned and shown to be identical to a DNA damage-inducible gene dinA which is regulated by the LexA repressor. Nucleotide sequencing of polB reveals that E coli DNA polymerase II is highly homologous to replicative DNA polymerases of ...
Shinagawa, H., Hiroshi Iwasaki, Ishino, Y., Nakata, A.   +3 more
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Transcription by RNA polymerase II: A process linked to DNA repair

BioEssays, 1994
AbstractThe proteins that are implicated in the basal transcription of protein coding genes have now been identified. Although little is known about their function, recent data demonstrate the ability of these proteins, previously called class II transcription factors, to participate in other reactions: TBP, the TATA‐box binding factor, is involved in ...
Chalut, Christian, Moncollin, Vincent, Egly, Jean Marc   +2 more
openaire   +5 more sources

Inhibition of DNA polymerases and DNA topoisomerase II by triterpenes produced by plant callus

Biochemical and Biophysical Research Communications, 2003
We found that some triterpene compounds could not only selectively inhibit the activities of mammalian DNA polymerase alpha (pol alpha) and beta (pol beta), but could also potently inhibit DNA topoisomerase II (topo II) [Biochem. J. 350 (2000) 757]. Here, we report that natural triterpenes produced by callus from an ancient Chinese medicinal plant were
Hiroshi Takazawa, Yoshiyuki Mizushina, Hiromi Yoshida, Kengo Sakaguchi, Kenji Endoh, Hiromasa Morita, Kohei Kamiya, Fumio Sugawara, Akira Ikuta, Masahiko Oshige, Toshiko Satake, Nobuyuki Kasai, Hiroaki Tomiyasu   +12 more
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Conformational change of DNA binding subunit of RNA polymerase II on binding to DNA

Biochemical and Biophysical Research Communications, 1985
The spatial arrangement of the subunits of RNA polymerase II from Ehrlich ascites tumor cells was investigated by measuring the sensitivity of each subunit in the native enzyme to various proteinases. The results showed that the largest two subunits (a and b) were sensitive to all the proteinases tested, whereas two smaller subunits (e and h) were ...
Kazuhisa Sekimizu, Shunji Natori, Masami Horikoshi   +2 more
openaire   +3 more sources

Escherichia coli DNA Polymerases II and III

1974
When cells of an E. coli mutant (pol Al−)1 which is deficient in DNA polymerase I (pol I) are lysed in a French pressure cell, they yield a cell-free extract with a small but demonstrable amount of DNA polymerizing activity.2 Following the removal of nucleic acid from such an extract, phosphocellulose chromatography can resolve two separate DNA ...
Thomas Kornberg, Thomas Kornberg, Ian J. Molineux, Ian J. Molineux, Malcolm L. Gefter, Malcolm L. Gefter   +5 more
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DNA Polymerases II and III of Escherichia coli

1974
Publisher Summary This chapter deals with the recent developments concerning the isolation and characterization of DNA polymerases II and III of Escherichia coli. Genetic and physiological experiments designed to elucidate the biological function of these enzymes are also discussed.
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Template properties of human dna‐dependent rna polymerase II

Medical and Pediatric Oncology, 1976
AbstractDNA‐dependent RNA polymerase II has been purified from lymphocytes of patients with chronic lymphocytic leukemia (CLL). Form II polymerase and Escherichia coli RNA polymerase have been used to study the transcription of human DNA and chromatin. The hybridization kinetics of the transcripts of DNA and chromatin by the human polymerase are quite ...
Grady F. Saunders, Ming‐Jer Tsai, Hector D. Garcia   +2 more
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Transcription of methylated viral DNA by eukaryotic RNA polymerase II

Cell Biophysics, 1989
The genome of the large icosahedral DNA virus, frog virus 3 (FV3), is heavily methylated at the cytosine residues of dCdG dinucleotide pairs, with more than 22% of the total cytosine residues in the form of 5-methylcytosine (5mC). This methylation is carried out postreplicatively in the cytoplasm of infected cells by a virus-encoded DNA ...
Rakesh Goorha, Dawn B. Willis, James P. Thompson, Karim Essani   +3 more
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DNA Polymerases of Chlorella. II. Characterization to Distinguish the Two Enzymes1

The Journal of Biochemistry, 1984
Previously, we reported two DNA polymerases (DPols), Pol I and Pol II, in the unicellular green alga, Chlorella (Aoshima, J., Nishimura, T., & Iwamura, T. (1982) Cell Struc. Funct. 7, 71-86). Changes in their activities during the cell cycle either in the normal and drug-inhibited courses indicated that Pol I and Pol II functioned to replicate nuclear ...
Takao Nishimura, Motoo Kubota, Jiro Aoshima, Tatsuichi Iwamura   +3 more
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Unwinding of DNA polymerases by the antitumor drug, cis-diamminedichloroplatinum(ii)

Chemical Communications, 2003
The helix-turn-helix motifs of the DNA binding domains of human polymerase-alpha and polymerase-kappa are dramatically perturbed upon binding to cisplatin with concomitant release of zinc.
Rathindra N. Bose, Wei W. Yang, Ferenc Evanics, Erika Volckova   +3 more
openaire   +3 more sources