Structural visualization of key steps in human transcription initiation. [PDF]
, 2013 Eukaryotic transcription initiation requires the assembly of general transcription factors into a pre-initiation complex that ensures the accurate loading of RNA polymerase II (Pol II) at the transcription start site. The molecular mechanism and function
Fang, Jie +3 more
core +1 more source
DNA dependent protein kinase (DNA-PK) enhances HIV transcription by promoting RNA polymerase II activity and recruitment of transcription machinery at HIV LTR. [PDF]
, 2020 Despite reductions in mortality from the use of highly active antiretroviral therapy (HAART), the presence of latent or transcriptionally silent proviruses prevents HIV cure/eradication.
Bukrinsky, Michael +10 more
core +2 more sources
Impact of template backbone heterogeneity on RNA polymerase II transcription. [PDF]
, 2014 Variations in the sugar component (ribose or deoxyribose) and the nature of the phosphodiester linkage (3'-5' or 2'-5' orientation) have been a challenge for genetic information transfer from the very beginning of evolution.
Chong, Jenny +5 more
core +1 more source
Modulation of RNA primer formation by Mn(II)-substituted T7 DNA primase
Scientific Reports, 2017 Lagging strand DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase. The N-terminal primase domain of the gene 4 protein of phage T7 comprises a zinc-binding domain that recognizes a specific DNA sequence and an RNA polymerase ...
Stefan Ilic +7 more
doaj +1 more source
Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis. [PDF]
, 2017 Most BRCA1-associated breast tumours are basal-like yet originate from luminal progenitors. BRCA1 is best known for its functions in double-strand break repair and resolution of DNA replication stress.
Angel Pujana, Miguel +29 more
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Monofunctional DNA-platinum(II) adducts block frequently DNA polymerases
Nucleic Acids Research, 1992 The question of whether monofunctional DNA platinum(II) adducts block synthesis of DNA by purified DNA polymerases of different types and origin has been investigated by comparing the time dependence of synthesis arrest and of DNA adduct formation.
E, Holler, R, Bauer, F, Bernges
openaire +3 more sources
COMPARISON OF SIX COMMERCIALLY-AVAILABLE DNA POLYMERASES FOR DIRECT PCR
Revista do Instituto de Medicina Tropical de São Paulo, 2013 SUMMARY The use of a “direct PCR” DNA polymerase enables PCR amplification without any prior DNA purification from blood samples due to the enzyme's resistance to inhibitors present in blood components. Such DNA polymerases are now commercially available.
Masashi Miura +3 more
doaj +1 more source
Inhibition of RNA polymerase II transcription in human cells by synthetic DNA-binding ligands [PDF]
, 1998 Sequence-specific DNA-binding small molecules that can permeate human cells potentially could regulate transcription of specific genes. Multiple cellular DNA-binding transcription factors are required by HIV type 1 for RNA synthesis.
Baird, Eldon E. +6 more
core +1 more source
PLoS ONE, 2014 Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle and RNA polymerase II mediated transcription. Several pharmacological CDK inhibitors are currently in clinical trials as potential cancer therapeutics and some of them also exhibit ...
Jitka Holcakova +8 more
doaj +1 more source
Life, 2022 RNAs not only offer valuable information regarding our bodies but also regulate cellular functions, allowing for their specific manipulations to be extensively explored for many different biological and clinical applications.
Hyesung Jo, Jiyun Beon, Seung Soo Oh
doaj +1 more source