Results 31 to 40 of about 812,933 (320)
RNA Pol II promotes transcription of centromeric satellite DNA in beetles. [PDF]
PLoS ONE, 2008 Transcripts of centromeric satellite DNAs are known to play a role in heterochromatin formation as well as in establishment of the kinetochore.
Zeljka Pezer, Durdica Ugarković
doaj +1 more source
Proofreading-defective DNA polymerase II increases adaptive mutation in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America, 1995 The role of Escherichia coli DNA polymerase (Pol) II in producing or avoiding mutations was investigated by replacing the chromosomal Pol II gene (polB+) by a gene encoding an exonuclease-deficient mutant Pol II (polBex1).
P. Foster +4 more
semanticscholar +1 more source
Modulation of RNA primer formation by Mn(II)-substituted T7 DNA primase
Scientific Reports, 2017 Lagging strand DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase. The N-terminal primase domain of the gene 4 protein of phage T7 comprises a zinc-binding domain that recognizes a specific DNA sequence and an RNA polymerase ...
Stefan Ilic +7 more
doaj +1 more source
Genetic dissection of the RNA polymerase II transcription cycle
eLife, 2022 How DNA sequence affects the dynamics and position of RNA Polymerase II (Pol II) during transcription remains poorly understood. Here, we used naturally occurring genetic variation in F1 hybrid mice to explore how DNA sequence differences affect the ...
Shao-Pei Chou +4 more
doaj +1 more source
COMPARISON OF SIX COMMERCIALLY-AVAILABLE DNA POLYMERASES FOR DIRECT PCR
Revista do Instituto de Medicina Tropical de São Paulo, 2013 SUMMARY The use of a “direct PCR” DNA polymerase enables PCR amplification without any prior DNA purification from blood samples due to the enzyme's resistance to inhibitors present in blood components. Such DNA polymerases are now commercially available.
Masashi Miura +3 more
doaj +1 more source
PLoS ONE, 2014 Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle and RNA polymerase II mediated transcription. Several pharmacological CDK inhibitors are currently in clinical trials as potential cancer therapeutics and some of them also exhibit ...
Jitka Holcakova +8 more
doaj +1 more source
Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae.
Nucleic Acids Research, 1991 DNA polymerase II purified from Saccharomyces cerevisiae contains polypeptides with apparent molecular masses of greater than 200, 80, 34, 30 and 29 kDa, the two largest of which (subunits A and B) are encoded by the essential genes POL2 and DPB2.
H. Araki +5 more
semanticscholar +1 more source
RNA Polymerase II Blockage by Cisplatin-damaged DNA [PDF]
Journal of Biological Chemistry, 2006 The consequences of human RNA polymerase II (pol II) arrest at the site of DNA damaged by cisplatin were studied in whole cells and cell extracts, with a particular focus on the stability of stalled pol II and its subsequent ubiquitylation. Site-specifically platinated DNA templates immobilized on a solid support were used to perform in vitro ...
Jung, YW Jung, Yongwon +1 more
openaire +4 more sources
Trajectory of DNA in the RNA polymerase II transcription preinitiation complex [PDF]
Proceedings of the National Academy of Sciences, 1997 By using site-specific protein-DNA photocrosslinking, we define the positions of TATA-binding protein, transcription factor IIB, transcription factor IIF, and subunits of RNA polymerase II (RNAPII) relative to promoter DNA within the human transcription preinitiation complex.
Richard H. Ebright +5 more
openaire +4 more sources
DNA polymerase ε associates with the elongating form of RNA polymerase II and nascent transcripts [PDF]
The FEBS Journal, 2006 DNA polymerase ε co‐operates with polymerases α and δ in the replicative DNA synthesis of eukaryotic cells. We describe here a specific physical interaction between DNA polymerase ε and RNA polymerase II, evidenced by reciprocal immunoprecipitation experiments.
Markku Vaara +11 more
openaire +3 more sources