Proofreading-defective DNA polymerase II increases adaptive mutation in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America, 1995 The role of Escherichia coli DNA polymerase (Pol) II in producing or avoiding mutations was investigated by replacing the chromosomal Pol II gene (polB+) by a gene encoding an exonuclease-deficient mutant Pol II (polBex1).
P. Foster +4 more
semanticscholar +1 more source
Modulation of RNA primer formation by Mn(II)-substituted T7 DNA primase
Scientific Reports, 2017 Lagging strand DNA synthesis by DNA polymerase requires RNA primers produced by DNA primase. The N-terminal primase domain of the gene 4 protein of phage T7 comprises a zinc-binding domain that recognizes a specific DNA sequence and an RNA polymerase ...
Stefan Ilic +7 more
doaj +1 more source
Computational Investigations on Polymerase Actions in Gene Transcription and Replication Combining Physical Modeling and Atomistic Simulations [PDF]
Chin. Phys. B Vol. 25, No. 1 (2016) 018706, 2015 Polymerases are protein enzymes that move along nucleic acid chains and catalyze template-based polymerization reactions during gene transcription and replication. The polymerases also substantially improve transcription or replication fidelity through the non-equilibrium enzymatic cycles.
arxiv +1 more source
RNA Pol II promotes transcription of centromeric satellite DNA in beetles. [PDF]
PLoS ONE, 2008 Transcripts of centromeric satellite DNAs are known to play a role in heterochromatin formation as well as in establishment of the kinetochore.
Zeljka Pezer, Durdica Ugarković
doaj +1 more source
Genetic dissection of the RNA polymerase II transcription cycle
eLife, 2022 How DNA sequence affects the dynamics and position of RNA Polymerase II (Pol II) during transcription remains poorly understood. Here, we used naturally occurring genetic variation in F1 hybrid mice to explore how DNA sequence differences affect the ...
Shao-Pei Chou +4 more
doaj +1 more source
Density- and elongation speed-dependent error correction in RNA polymerization [PDF]
, 2021 Backtracking of RNA polymerase (RNAP) is an important pausing mechanism during DNA transcription that is part of the error correction process that enhances transcription fidelity. We model the backtracking mechanism of RNA polymerase, which usually happens when the polymerase tries to incorporate a mismatched nucleotide triphosphate.
arxiv +1 more source
COMPARISON OF SIX COMMERCIALLY-AVAILABLE DNA POLYMERASES FOR DIRECT PCR
Revista do Instituto de Medicina Tropical de São Paulo, 2013 SUMMARY The use of a “direct PCR” DNA polymerase enables PCR amplification without any prior DNA purification from blood samples due to the enzyme's resistance to inhibitors present in blood components. Such DNA polymerases are now commercially available.
Masashi Miura +3 more
doaj +1 more source
The Escherichia coli polB gene, which encodes DNA polymerase II, is regulated by the SOS system
Journal of Bacteriology, 1990 The dinA (damage inducible) gene was previously identified as one of the SOS genes with no known function; it was mapped near the leuB gene, where the polB gene encoding DNA polymerase II was also mapped.
H. Iwasaki +3 more
semanticscholar +1 more source
Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation. [PDF]
PLoS ONE, 2010 DNA cytosine methylation is an important epigenetic mechanism that is involved in transcriptional silencing of developmental genes. Several molecular pathways have been described that interfere with Pol II initiation, but at individual genes the ...
Yongguang Tao +3 more
doaj +1 more source
RNA Polymerase II Blockage by Cisplatin-damaged DNA [PDF]
Journal of Biological Chemistry, 2006 The consequences of human RNA polymerase II (pol II) arrest at the site of DNA damaged by cisplatin were studied in whole cells and cell extracts, with a particular focus on the stability of stalled pol II and its subsequent ubiquitylation. Site-specifically platinated DNA templates immobilized on a solid support were used to perform in vitro ...
Jung, YW Jung, Yongwon +1 more
openaire +4 more sources