Results 51 to 60 of about 1,237,824 (318)
MethodsX, 2021 Analyses of environmental DNA (eDNA) from macroorganisms in aquatic environments have greatly advanced in recent years. In particular, eDNA metabarcoding of fish using universal PCR primers has been reported in various waters.
Masaru Kawato +8 more
doaj +1 more source
Mechanistic insights into the transcriptional arrest in the presence of Double Strand Breaks [PDF]
, 2014 Double-strand breaks (DSBs) occur frequently in the genome during genome replication or by DNA damaging agents. DNA lesions affect fundamental DNA-dependent nuclear processes, such as replication and transcription.
Bonhomme, Celine +6 more
core +2 more sources
DNA-Dependent DNA Polymerases I and II from Normal Human-Blood Lymphocytes [PDF]
Proceedings of the National Academy of Sciences, 1972 Two DNA polymerases have been purified from blood lymphocytes of normal human individuals. In contrast to a DNA polymerase isolated from lymphoblasts from individuals with leukemia, neither of the DNA polymerases from normal lymphocytes transcribes the heteropolymeric regions of tumor-virus RNA into DNA.
R G, Smith, R C, Gallo
openaire +2 more sources
Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity [PDF]
Open Biology, 2017 During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase ...
Vasileios Paschalis +5 more
doaj +1 more source
RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
eLife, 2023 Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation ...
James ZJ Kwan +8 more
doaj +1 more source
Journal of Biological Chemistry, 1995 Wild-type DNA polymerase II (pol II) and an exonuclease-deficient pol II mutant (D155A/E157A) have been overexpressed and purified in high yield from Escherichia coli.
Hong Cai +4 more
semanticscholar +1 more source
Journal of Biological Chemistry, 1988 The newly identified yeast DNA polymerase III was compared to DNA polymerases I and II and the mitochondrial DNA polymerase. Inhibition by aphidicolin (I50) of DNA polymerases I, II, and III was 4, 6, and 0.6 micrograms/ml, respectively. The mitochondrial enzyme was insensitive to the drug.
P M, Burgers, G A, Bauer
openaire +2 more sources
Shiyan dongwu yu bijiao yixue, 2023 Objective To establish a set of single nucleotide polymorphisms (SNP) detection protocol for inbred rats based on multiplex PCR-ligase detection reaction (LDR).Methods A total of 40 rats SNP sites were selected on chromosomes 1-20 and X of rats among 5 ...
ZHAO Liya +7 more
doaj +1 more source
Journal of Biological Chemistry, 1998 Two forms of DNA polymerase II (ε) ofSaccharomyces cerevisiae, Pol II* and Pol II, were purified to near homogeneity from yeast cells. Pol II* is a four-subunit complex containing a 256-kDa catalytic polypeptide, whereas Pol II consists solely of a 145 ...
S. Maki +3 more
semanticscholar +1 more source
The trans-activation domain of the sporulation response regulator Spo0A revealed by X-ray crystallography [PDF]
, 2000 Sporulation in Bacillus involves the induction of scores of genes in a temporally and spatially co-ordinated programme of cell development. Its initiation is under the control of an expanded two-component signal transduction system termed a phosphorelay.
Baldus J.M. +32 more
core +1 more source