Results 51 to 60 of about 900,531 (345)

DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America, 1991
The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second
H. Araki, R. Hamatake, L. Johnston, A. Sugino   +3 more
semanticscholar   +1 more source

Interactions of the Bacillus subtilis DnaE polymerase with replisomal proteins modulate its activity and fidelity [PDF]

Open Biology, 2017
During Bacillus subtilis replication two replicative polymerases function at the replisome to collectively carry out genome replication. In a reconstituted in vitro replication assay, PolC is the main polymerase while the lagging strand DnaE polymerase ...
Vasileios Paschalis, Emmanuelle Le Chatelier, Matthew Green, François Képès, Panos Soultanas, Laurent Janniere   +5 more
doaj   +1 more source

RNA Polymerase II transcription independent of TBP in murine embryonic stem cells

eLife, 2023
Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation ...
James ZJ Kwan, Thomas F Nguyen, Anuli C Uzozie, Marek A Budzynski, Jieying Cui, Joseph MC Lee, Filip Van Petegem, Philipp F Lange, Sheila S Teves   +8 more
doaj   +1 more source

First-passage problems in DNA replication: effects of template tension on stepping and exonuclease activities of a DNA polymerase motor [PDF]

Journal of Physics: Condensed Matter, vol.25, 374105 (2013), 2013
A DNA polymerase (DNAP) replicates a template DNA strand. It also exploits the template as the track for its own motor-like mechanical movement. In the polymerase mode it elongates the nascent DNA by one nucleotide in each step. But, whenever it commits an error by misincorporating an incorrect nucleotide, it can switch to an exonuclease mode.
arxiv   +1 more source

Aphidicolin inhibits DNA polymerase II ofEscherichia coli, an alpha-like DNA polymerase

Nucleic Acids Research, 1990
The polB gene of E. coli encodes DNA polymerase II. We have reported the cloning (1,2) and sequencing of the polB gene (3) (GenBank Accession number M35371). DNA polymerase II appears to be a non-essential function since the polB gene is missing from some widely used strains (3). Several sub-families of DNA polymerases have been identified (4). In this
Robb E. Moses, Charles B. Lawrence, Hong Chen, S.K. Bryan   +3 more
openaire   +3 more sources

DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli

Molecular Microbiology, 2005
Escherichia coli DNA polymerase III holoenzyme (HE) is the main replicase responsible for replication of the bacterial chromosome. E. coli contains four additional polymerases, and it is a relevant question whether these might also contribute to ...
Magdalena Banach-Orłowska, I. Fijalkowska, R. Schaaper, P. Jonczyk   +3 more
semanticscholar   +1 more source

HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia

Nature Communications, 2022
Hypoxia-inducible factor-1 (HIF-1) is a transcription factor that modulates target gene expression in response to changes in oxygen availability. Here the authors show that HIF-1 forms a complex with TRIM28 and DNA-dependent protein kinase (DNA-PK) that ...
Yongkang Yang, Haiquan Lu, Chelsey Chen, Yajing Lyu, Robert N. Cole, Gregg L. Semenza   +5 more
doaj   +1 more source

Stochastic resetting in backtrack recovery by RNA polymerases [PDF]

Phys. Rev. E 93, 062411 (2016), 2016
Transcription is a key process in gene expression, in which RNA polymerases produce a complementary RNA copy from a DNA template. RNA polymerization is frequently interrupted by backtracking, a process in which polymerases perform a random walk along the DNA template.
arxiv   +1 more source

Involvement of Escherichia coli DNA polymerase II in response to oxidative damage and adaptive mutation

Journal of Bacteriology, 1994
DNA polymerase II (Pol II) is regulated as part of the SOS response to DNA damage in Escherichia coli. We examined the participation of Pol II in the response to oxidative damage, adaptive mutation, and recombination.
M. Escarceller, '. J. Hicks, G. Gudmundsson, '. G. Trump, D. Touati, S. Lovet, P. Foster, K. McEntee, F. Myron, '. Goodman   +9 more
semanticscholar   +1 more source

Establishing a Genetic Detection Protocol of Single Nucleotide Polymorphisms Panels in Inbred Rats Based on Multiplex PCR-LDR

Shiyan dongwu yu bijiao yixue, 2023
Objective To establish a set of single nucleotide polymorphisms (SNP) detection protocol for inbred rats based on multiplex PCR-ligase detection reaction (LDR).Methods A total of 40 rats SNP sites were selected on chromosomes 1-20 and X of rats among 5 ...
ZHAO Liya, NI Liju, ZHANG Caiqin, TANG Jianping, YAO Yangzheng, NIE Yanyan, GU Xiaoxue, ZHAO Ying   +7 more
doaj   +1 more source