Results 61 to 70 of about 812,933 (320)
An endonuclease closely associated with DNA‐dependent RNA polymerase II [PDF]
FEBS Letters, 1985 An endonuclease tightly bound to RNA polymerase II has been purified. The enzyme consists of two subunits, and is essential for the polymerase activity.
Jan Szopa +3 more
openaire +2 more sources
A stepwise emergence of evolution in the RNA world
FEBS Letters, EarlyView.How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley +1 more source
Escherichia coli DNA polymerase II is stimulated by DNA polymerase III holoenzyme auxiliary subunits
Journal of Biological Chemistry, 1991 DNA polymerase III of Escherichia coli requires multiple auxiliary factors to enable it to serve as a replicative complex. We demonstrate that auxiliary components of the DNA polymerase III holoenzyme, the gamma delta complex and beta subunit, markedly stimulate DNA polymerase II on long single-stranded templates. DNA polymerase II activity is enhanced
Hong Chen +4 more
openaire +3 more sources
FEBS Letters, EarlyView.The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp +5 more
wiley +1 more source
Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.
PLoS ONE, 2010 DNA cytosine methylation is an important epigenetic mechanism that is involved in transcriptional silencing of developmental genes. Several molecular pathways have been described that interfere with Pol II initiation, but at individual genes the ...
Yongguang Tao +3 more
doaj +1 more source
Loci-specific phase separation of FET fusion oncoproteins promotes gene transcription
Nature Communications, 2021 FUS/EWS/TAF15 (FET) fusion oncoproteins contain low complexity domain which forms biomolecular condensates that recruit RNA polymerase II. Here the authors develop a single-molecule assay to visualize this phenomenon providing in vitro evidence to ...
Linyu Zuo +10 more
doaj +1 more source
Amitochondriate amoebae and the evolution of DNA-dependent RNA polymerase II [PDF]
Proceedings of the National Academy of Sciences, 1998 Unlike parasitic protist groups that are defined by the absence of mitochondria, the Pelobiontida is composed mostly of free-living species. Because of the presence of ultrastructural and cellular features that set them apart from all other eukaryotic organisms, it has been suggested that pelobionts are primitively amitochondriate and may ...
Ellen C. S. Duffield +2 more
openaire +3 more sources
FEBS Letters, EarlyView.UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg +2 more
wiley +1 more source
Comparison of Three RNA Amplification Methods as Sources of DNA for Sequencing
BioTechniques, 1998 DNA products generated from a region of the measles virus genome by three RNA reverse transcription and amplification methods were cloned and sequenced, and the results were compared in order to evaluate the methods’ relative fidelities. The methods were:
Nicholas Chadwick +3 more
doaj +1 more source
The Mediator complex as a master regulator of transcription by RNA polymerase II
Nature reviews. Molecular cell biology, 2022 The Mediator complex, which in humans is 1.4 MDa in size and includes 26 subunits, controls many aspects of RNA polymerase II (Pol II) function. Apart from its size, a defining feature of Mediator is its intrinsic disorder and conformational flexibility,
William F. Richter +3 more
semanticscholar +1 more source