Results 71 to 80 of about 900,531 (345)
Error correction during DNA replication [PDF]
Physical Review E, VOL.86, 011913 (2012), 2011 DNA polymerase (DNAP) is a dual-purpose enzyme that plays two opposite roles in two different situations during DNA replication. It plays its normal role as a {\it polymerase} catalyzing the elongation of a new DNA molecule by adding a monomer. However, it can switch to the role of an {\it exonuclease} and shorten the same DNA by cleavage of the last ...
arxiv +1 more source
Journal of Biological Chemistry, 1998 Two forms of DNA polymerase II (ε) ofSaccharomyces cerevisiae, Pol II* and Pol II, were purified to near homogeneity from yeast cells. Pol II* is a four-subunit complex containing a 256-kDa catalytic polypeptide, whereas Pol II consists solely of a 145 ...
S. Maki +3 more
semanticscholar +1 more source
Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells
FEBS Letters, EarlyView.Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga +6 more
wiley +1 more source
PLoS Genetics, 2019 Repetitive DNA sequences within eukaryotic heterochromatin are poorly transcribed and replicate late in S-phase. In Saccharomyces cerevisiae, the histone deacetylase Sir2 is required for both transcriptional silencing and late replication at the ...
Eric J Foss +7 more
doaj +1 more source
Transcriptional regulation: Effects of promoter proximal pausing on speed, synchrony and reliability [PDF]
PLoS Comput Biol 7(5): e1001136 (2011), 2011 Recent whole genome polymerase binding assays have shown that a large proportion of unexpressed genes have pre-assembled RNA pol II transcription initiation complex stably bound to their promoters. Some such promoter proximally paused genes are regulated at transcription elongation rather than at initiation; it has been proposed that this difference ...
arxiv +1 more source
Nucleic Acids Research, 1999 Pyrococcus furiosus has an operon containing the DNA polymerase II (PolD) gene and three other genes. Using a two-hybrid screening to examine the interactions of the proteins encoded by the operon, we identified a specific interaction between the second ...
I. Hayashi, K. Morikawa, Y. Ishino
semanticscholar +1 more source
FEBS Letters, EarlyView.The S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Laetitia Gilet +4 more
wiley +1 more source
Comparison of Three RNA Amplification Methods as Sources of DNA for Sequencing
BioTechniques, 1998 DNA products generated from a region of the measles virus genome by three RNA reverse transcription and amplification methods were cloned and sequenced, and the results were compared in order to evaluate the methods’ relative fidelities. The methods were:
Nicholas Chadwick +3 more
doaj +1 more source
Escherichia coli DNA polymerase II is stimulated by DNA polymerase III holoenzyme auxiliary subunits
Journal of Biological Chemistry, 1991 DNA polymerase III of Escherichia coli requires multiple auxiliary factors to enable it to serve as a replicative complex. We demonstrate that auxiliary components of the DNA polymerase III holoenzyme, the gamma delta complex and beta subunit, markedly stimulate DNA polymerase II on long single-stranded templates. DNA polymerase II activity is enhanced
Hong Chen +4 more
openaire +3 more sources
An endonuclease closely associated with DNA‐dependent RNA polymerase II [PDF]
FEBS Letters, 1985 An endonuclease tightly bound to RNA polymerase II has been purified. The enzyme consists of two subunits, and is essential for the polymerase activity.
Jan Szopa +3 more
openaire +2 more sources