Results 101 to 110 of about 3,903,497 (411)

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

open access: yesFEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp   +5 more
wiley   +1 more source

Detection of rtxA Gene as a Biomarker of Seafood-Borne Pathogen Vibrio cholerae using In Silico PCR Assay

open access: yesSqualen, 2020
Seafood-borne outbreaks caused by Vibrio cholerae have led to the increased need for food safety risk assessment of marine products. An in silico investigation about the potential of virulence gene of V.
Stalis Norma Ethica   +6 more
doaj   +1 more source

DNA fingerprinting of red jungle fowl, village chicken and broilers [PDF]

open access: yes, 2000
The genomic mapping of Red Jungle Fowl (Gallus gallus), local Village Chicken, and broiler was carried out by random amplified polymorphism DNA (RAPD) technique. Two different sets of arbitrary primers were used (Operon OPAO1-20 and Genemed GM01-50). All
Ali, A. S.   +2 more
core   +1 more source

Brucella NyxA and NyxB dimerization enhances effector function during infection

open access: yesFEBS Letters, EarlyView.
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre   +4 more
wiley   +1 more source

Analysis of Metagenome Composition by the Method of Random Primers [PDF]

open access: yesarXiv, 2016
Metagenome, a mixture of different genomes (as a rule, bacterial), represents a pattern, and the analysis of its composition is, currently, one of the challenging problems of bioinformatics. In the present study, the possibility of evaluating metagenome composition by DNA-marker methods is investigated.
arxiv  

Development of A PCR - Cycle Sequencing System for Animal Mitochondrial DNA Analysis [PDF]

open access: yes, 1994
A system for the study of sequence polymorphisms in animal mitochondrial DNA was developed. This system consists of two candidate markers which are mitochondrial cytochrome b and D-loop.
Lau, Chin Hoon
core  

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

open access: yesFEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg   +2 more
wiley   +1 more source

Constructing a DNA ladder Range for Lambda Phage by multiplex PCR

open access: yesIranian Journal of Microbiology, 2010
Background and Objectives: DNA ladder contains DNA fragments of different length but with known size, used to determine the size of unknown DNA molecules. Different DNA ladders are available for expected DNA length.
R Gopalakrishnan, S Joseph, S Sellappa
doaj  

Efficient and Explicit Balanced Primer Codes [PDF]

open access: yesarXiv, 2019
To equip DNA-based data storage with random-access capabilities, Yazdi et al. (2018) prepended DNA strands with specially chosen address sequences called primers and provided certain design criteria for these primers. We provide explicit constructions of error-correcting codes that are suitable as primer addresses and equip these constructions with ...
arxiv  

Designing of primer for PCR [PDF]

open access: yes, 2003
The polymerase chain reaction (PCR) is the technique for in vitro synthesis of multiple copies of a given DNA molecule. Primers are the most important components of PCR and success of PCR largely depends on the primers. A primer is an oligonucleotide. It
Thomas, P C
core  

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