Results 101 to 110 of about 1,066,757 (310)

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Ciliate DNA barcoding [PDF]

open access: yes, 2012
DNA barcoding is a taxonomic method that uses a short genetic marker in an organisms DNA to identify it as belonging to a particular species. A desirable gene region for DNA barcoding should be standardized, present in most of the taxa of interest and ...
김세주, 민기식
core  

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells [PDF]

open access: yes, 2005
Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström   +16 more
core   +1 more source

Identification of MADS-box Gene in Oil Palm (Elaeis guineensis Jacq.)

open access: yesMakara Journal of Science, 2016
The bunch size represented by the fruit number is the main parameter of oil palm (Elaeis guineensis Jacq.) yield. The fruit number, which is determined during the initial phase of development, is related to various factors, including the genetic ...
Winda Nawfetrias   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

open access: yesFEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj   +3 more
wiley   +1 more source

Mutually Uncorrelated Primers for DNA-Based Data Storage

open access: yesIEEE Transactions on Information Theory, 2018
14 pages, 3 figures, 1 Table.
S. M. Hossein Tabatabaei Yazdi   +3 more
openaire   +3 more sources

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

open access: yesFEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile   +8 more
wiley   +1 more source

DNA primers used for site-directed mutagenesis. [PDF]

open access: yes, 2016
DNA primers used for site-directed mutagenesis.
Ruiping Cai (3161421)   +6 more
core   +1 more source

Conserve primers for sequencing complete ungulate mitochondrial cytochrome c oxidase I (COI) gene from problematic and decomposed biological samples

open access: yesMitochondrial DNA. Part B. Resources, 2017
We describe six novel ungulate-specific conserved primers for sequencing the complete mitochondrial cytochrome c oxidase I (COI) gene of selected threatened species from degraded samples for effective conservation planning.
Ajit Kumar   +5 more
doaj   +1 more source

PCR-based identification of toxinogenic Fusarium species

open access: yesCzech Journal of Food Sciences, 2009
Aim of this study was to develop sensitive PCR assay for mycotoxin producing Fusarium species. Strains Fusarium oxysporum 4199 and Fusarium culmorum 4044 were used as representatives of this group.
Iva Lacmanová   +3 more
doaj   +1 more source

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