Results 141 to 150 of about 3,903,497 (411)

Natural site-directed mutagenesis might exist in eukaryotic cells [PDF]

arXiv, 2016
Site-directed mutagenesis refers to a man-made molecular biology method that is used to make genetic alterations in the DNA sequence of a gene of interest. But based on our recently published experimental findings, we propose that natural site-directed mutagenesis might exist in the eukaryotic cells, which is triggered by harmful agents and co-directed
arxiv  

Pcr Primers for the Amplification of Mitochondrial Small Subunit Ribosomal DNA of Lichen-forming Ascomycetes [PDF]

, 2017
Four primers for the amplification of mitochondrial DNA of lichenforming ascomycetes are presented. The primers match the conserved regions U2, U4, and U6, respectively, of mitochondrial small subunit (SSU) ribosomal DNA (rDNA). Polymerase chain reaction
Scheidegger, Christoph, Sperisen, Christoph, Zoller, Stefan   +2 more
core  

KRAS and GNAS mutations in cell‐free DNA and in circulating epithelial cells in patients with intraductal papillary mucinous neoplasms—an observational pilot study

Molecular Oncology, EarlyView.
This study demonstrates that KRAS and GNAS mutations are more prevalent in patients with resected intraductal papillary mucinous neoplasms (IPMN) compared to those under clinical surveillance. GNAS mutations significantly differ between the two patient cohorts, indicating that their absence may serve as a potential biomarker to support conservative ...
Christine Nitschke, Marie Tölle, Philipp Walter, Kira Meißner, Mara Goetz, Jolanthe Kropidlowski, Andreas W. Berger, Jakob R. Izbicki, Felix Nickel, Thilo Hackert, Klaus Pantel, Harriet Wikman, Faik G. Uzunoglu   +12 more
wiley   +1 more source

A rapid molecular approach to determining the occurrence of pathogen indicators in compost [PDF]

arXiv, 2014
An accurate method for enumerating pathogen indicators, such as Escherichia coli (E. coli), and Salmonella spp. is important for assessing the safety of compost samples. This study aimed to determine the occurrence of pathogen indicators in compost samples by using a molecular approach known as Polymerase Chain Reaction (PCR).
arxiv  

Primer-independent DNA polymerases and their use for DNA synthesis

, 2018
Consejo Superior de Investigaciones Científicas (España), Institut ...
Salas, Margarita, Redrejo-Rodríguez, Modesto, Krupovic, Mart, Forterre, Patrick   +3 more
openaire   +3 more sources

Primer removal during mammalian mitochondrial DNA replication

DNA Repair, 2015
The small circular mitochondrial genome in mammalian cells is replicated by a dedicated replisome, defects in which can cause mitochondrial disease in humans. A fundamental step in mitochondrial DNA (mtDNA) replication and maintenance is the removal of the RNA primers needed for replication initiation. The nucleases RNase H1, FEN1, DNA2, and MGME1 have
Maria Falkenberg, Jay P. Uhler
openaire   +3 more sources

Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

mSystems, 2015
We continue to uncover a wealth of information connecting microbes in important ways to human and environmental ecology. As our scientific knowledge and technical abilities improve, the tools used for microbiome surveys can be modified to improve the ...
William Walters, Embriette R. Hyde, Donna berg-Lyons, Gail L Ackermann, G. Humphrey, A. Parada, J. Gilbert, J. Jansson, J. Caporaso, J. Fuhrman, A. Apprill, R. Knight   +11 more
semanticscholar   +1 more source

Cellular liquid biopsy provides unique chances for disease monitoring, preclinical model generation and therapy adjustment in rare salivary gland cancer patients

Molecular Oncology, EarlyView.
We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić, Florian Lüke, Steffi Treitschke, Andrea Coluccio, Martin Hoffmann, Giancarlo Feliciello, Adithi Ravikumar Varadarajan, Xin Lu, Kathrin Weidele, Catherine Botteron, Silvia Materna–Reichelt, Felix Keil, Katja Evert, Florian Weber, Thomas Schamberger, Michael Althammer, Jirka Grosse, Dirk Hellwig, Christian Schulz, Stephan Seitz, Peter Ugocsai, Anke Schlenska‐Lange, Roman Mayr, Ulrich Kaiser, Wolfgang Dietmaier, Bernhard Polzer, Jens Warfsmann, Kamran Honarnejad, Tobias Pukrop, Daniel Heudobler, Christoph A. Klein, Christian Werno   +31 more
wiley   +1 more source

SELEÇÃO DE PRIMERS ISSR PARA ANÁLISES DE DIVERSIDADE GENÉTICA EM Plathymenia reticulata Benth

Revista UniVap, 2017
O objetivo deste trabalho foi selecionar primers ISSR para serem utilizados em futuras análises de diversidade genética em populações naturais de P. reticulata. Amostras foliares de cinco indivíduos da espécie foram coletadas para extração e purificação
Lucimara Cruz de Souza, Adelson Lemes da Silva Júnior, Aléxia Gonçalves Pereira, Mariana Cruz de Souza, Kelmer Mozer Moro, Alessandra Abreu Rodrigues Vieira, Izabela Ferreira Ribeiro, Sustanis Horn Kunz, Fábio Demolinari de Miranda   +8 more
doaj   +1 more source

Collision Aware Data Allocation In Multi-tube DNA Storage [PDF]

arXiv
DNA storage is a promising archival data storage solution to today's big data problem. A DNA storage system encodes and stores digital data with synthetic DNA sequences and decodes DNA sequences back to digital data via sequencing. For efficient target data retrieving, existing Polymerase Chain Reaction (PCR) based DNA storage systems apply primers as ...
arxiv