Results 231 to 240 of about 1,454,497 (332)

Borrelia burgdorferi gene encoding conserved 79 kDa antigen: DNA probe or DNA primer and polymerase chain reaction for Lyme disease diagnosis and recombinant vaccine; monoclonal antibody production

, 1993

openalex   +1 more source

Interdependence between DNA template secondary structure and priming efficiencies of short primers [PDF]

, 1998
L. Lvovsky, Ilya Ioshikhes, M. C. Raja, D. Zevin‐Sonkin, Ivan Sobolev, Arthur Liberzon, J. Shwartzburd, Levy Ulanovsky   +7 more
openalex   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Nested PCR to optimize <i>rpoB</i> metabarcoding for low-concentration and host-associated bacterial DNA. [PDF]

Microbiol Spectr
Leclerc L, Agoutin G, Brévault T, Champion A, Mainguy J, Nègre N, Yainna S, Pascal G, Gaudriault S, Ogier J-C.   +9 more
europepmc   +1 more source

Characterization of WAC interactions with R2TP and TTT chaperone complexes linking glucose and glutamine availability to mTORC1 activity

FEBS Open Bio, EarlyView.
TTT and R2TP chaperone complexes are required for the assembly and activation of mTORC1. WAC directly interacts with components of TTT, R2TP, and mTORC1, and these interactions are affected by the availability of glucose and glutamine, correlating with changes in mTORC1 activity.
Sofía Cabezudo, Natalia Cuervo, Carmen García‐Martín, Andrés López‐Perrote, Clara Reglero, Adrián Maqueda‐Real, Ana González‐Corpas, Marina Serna, Diego Megías, Alejo Efeyan, Solip Park, Oscar Llorca   +11 more
wiley   +1 more source

Loop mediated isothermal amplification (LAMP) as a rapid and portable diagnostic tool for the detection of pea root rot pathogens. [PDF]

Sci Rep
Pacheco-Moreno A, Bruty S, Tidd H, Morka E, Arora S.   +4 more
europepmc   +1 more source

Photoaffinity labeling of DNA template-primer binding site in Escherichia coli DNA polymerase I. Identification of involved amino acids. [PDF]

, 1995
Virendra N. Pandey, Neerja Kaushik, Mukund J. Modak   +2 more
openalex   +1 more source

Two‐way inhibition of PAX5 transcriptional activity by PAX5::CBFA2T3

FEBS Open Bio, EarlyView.
PAX5::CBFA2T3 (PAX5‐C) is a fusion protein of the B‐cell transcription factor, PAX5, and is found in B‐cell ALL. We propose a putative model of two‐way inhibition of PAX5 transcriptional activity by PAX5‐C. There are two ways of repression by PAX5‐C: DNA‐binding‐dependent way and HDAC‐dependent way, with either being sufficient for the repression. HDAC
Reina Ueno, Aki Terasaki, Yuiko Imai, Yuri Kimura, Yuna Kojima, Misa Irie, Koya Odaira, Mina Noura, Shuichi Okamoto, Takahiko Yasuda, Shinobu Tsuzuki, Hitoshi Kiyoi, Fumihiko Hayakawa   +12 more
wiley   +1 more source

Field-Deployable Detection of Chestnut Blight Pathogen <i>Cryphonectria parasitica</i> Using Enzyme-Mediated Duplex Exponential Amplification. [PDF]

Curr Issues Mol Biol
Wang S, Feng Z, Liu Y, Tang C, Guo K, Hu J.   +5 more
europepmc   +1 more source

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

FEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli, Sophie Valière, Julien Sarry, Cécile Donnadieu, Julie Demars   +4 more
wiley   +1 more source