Results 131 to 140 of about 3,796,516 (329)

DNA Probes for Cas12a-Based Assay with Fluorescence Anisotropy Enhanced Due to Anchors and Salts. [PDF]

Biosensors (Basel), 2023
Safenkova IV, Samokhvalov AV, Serebrennikova KV, Eremin SA, Zherdev AV, Dzantiev BB.   +5 more
europepmc   +1 more source

Electrical Probes of DNA-Binding Proteins [PDF]

, 2017
A DNA electrochemistry platform has been developed to probe proteins bound to DNA electrically. Here gold electrodes are modified with thiol-modified DNA, and DNA charge transport chemistry is used to probe DNA binding and enzymatic reaction both with redox-silent and redox-active proteins.
Barton, Jacqueline K., Bartels, Phillip L., Deng, Yingxin, O'Brien, Elizabeth   +3 more
openaire   +5 more sources

On‐treatment dynamics of circulating extracellular vesicles in the first‐line setting of patients with advanced non‐small cell lung cancer: the LEXOVE prospective study

Molecular Oncology, EarlyView.
The LEXOVE prospective study evaluated plasma cell‐free extracellular vesicle (cfEV) dynamics using Bradford assay and dynamic light scattering in metastatic non‐small cell lung cancer patients undergoing first‐line treatments, correlating a ∆cfEV < 20% with improved median progression‐free survival in responders versus non‐responders.
Valerio Gristina, Viviana Bazan, Nadia Barraco, Simona Taverna, Mauro Manno, Samuele Raccosta, Anna Paola Carreca, Marco Bono, Tancredi Didier Bazan Russo, Francesco Pepe, Pasquale Pisapia, Lorena Incorvaia, Giuseppe Badalamenti, Giancarlo Troncone, Umberto Malapelle, Daniele Santini, Antonio Russo, Antonio Galvano   +17 more
wiley   +1 more source

Chemically programmable bacterial probes for the recognition of cell surface proteins

Materials Today Bio, 2023
Common methods to label cell surface proteins (CSPs) involve the use of fluorescently modified antibodies (Abs) or small-molecule-based ligands. However, optimizing the labeling efficiency of such systems, for example, by modifying them with additional ...
Pragati K. Prasad, Noa Eizenshtadt, Inna Goliand, Liat Fellus-Alyagor, Roni Oren, Ofra Golani, Leila Motiei, David Margulies   +7 more
doaj  

A Critical Study on DNA Probes Attached to Microplate for CRISPR/Cas12 Trans-Cleavage Activity. [PDF]

Biosensors (Basel), 2023
Burkin KM, Ivanov AV, Zherdev AV, Dzantiev BB, Safenkova IV.   +4 more
europepmc   +1 more source

Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes

Applied and Environmental Microbiology, 2006
Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted ...
K. Kubota, A. Ohashi, H. Imachi, H. Harada   +3 more
semanticscholar   +1 more source

Cellular liquid biopsy provides unique chances for disease monitoring, preclinical model generation and therapy adjustment in rare salivary gland cancer patients

Molecular Oncology, EarlyView.
We quantified and cultured circulating tumor cells (CTCs) of 62 patients with various cancer types and generated CTC‐derived tumoroid models from two salivary gland cancer patients. Cellular liquid biopsy‐derived information enabled molecular genetic assessment of systemic disease heterogeneity and functional testing for therapy selection in both ...
Nataša Stojanović Gužvić, Florian Lüke, Steffi Treitschke, Andrea Coluccio, Martin Hoffmann, Giancarlo Feliciello, Adithi Ravikumar Varadarajan, Xin Lu, Kathrin Weidele, Catherine Botteron, Silvia Materna–Reichelt, Felix Keil, Katja Evert, Florian Weber, Thomas Schamberger, Michael Althammer, Jirka Grosse, Dirk Hellwig, Christian Schulz, Stephan Seitz, Peter Ugocsai, Anke Schlenska‐Lange, Roman Mayr, Ulrich Kaiser, Wolfgang Dietmaier, Bernhard Polzer, Jens Warfsmann, Kamran Honarnejad, Tobias Pukrop, Daniel Heudobler, Christoph A. Klein, Christian Werno   +31 more
wiley   +1 more source

Imaging and detecting intercellular tensile forces in spheroids and embryoid bodies using lipid-modified DNA probes. [PDF]

Front Cell Dev Biol, 2023
Tian Q, Yang F, Jiang H, Bhattacharyya P, Xie T, Ali AA, Sun Y, You M.   +7 more
europepmc   +1 more source

DNA-probes for the differentiation ofCapnocytophagaspecies

Molecular and Cellular Probes, 1997
We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical Capnocytophaga isolates as well
Conrads, Georg, Mutters, Reinier, Seyfarth, I., Pelz, Klaus   +3 more
openaire   +4 more sources

Novel DNA probes with low background and high hybridization-triggered fluorescence

Nucleic Acids Research, 2007
Novel fluorogenic DNA probes are described. The probes (called Pleiades) have a minor groove binder (MGB) and a fluorophore at the 5′-end and a non-fluorescent quencher at the 3′-end of the DNA sequence.
E. Lukhtanov, S. Lokhov, V. Gorn, M. Podyminogin, W. Mahoney   +4 more
semanticscholar   +1 more source