Results 231 to 240 of about 3,609,377 (271)

Internal Labeling of DNA Probes

2003
Ralph Rapley and BimalD.M. Theophilus, 'Internal labeling of DNA Probes' in R. Rapley and B. D. M. Theophilus, eds., PCR Mutation Detection Protocols (Totowa: Humana Press 2002) ISBN: 978-0-89603-617-8, eISBN: 978-1-59259-273-9, DOI: 10.1385/1-59259-273-2:013 © 2002 Humana Press Inc., Totowa, NJ.
Bimal D. M. Theophilus, Ralph Rapley
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Diagnostic Applications of DNA Probes [PDF]

Infection Control & Hospital Epidemiology, 1991
The use of recombinant DNA technology to develop specific molecular (usually DNA) probes has provided medical and veterinary diagnostic laboratories with powerful new tools to enhance the diagnosis of infectious diseases, genetic disorders, and malignancies, as well as more sensitive and specific means to accomplish such tasks as tissue typing and ...
openaire   +2 more sources

Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection.

ACS Nano, 2012
The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated ...
Pratik Shah, Andreas Rørvig-Lund, S. Chaabane, P. Thulstrup, H. Kjaergaard, E. Fron, J. Hofkens, S. Yang, T. Vosch   +8 more
semanticscholar   +1 more source

Graphene oxide-protected DNA probes for multiplex microRNA analysis in complex biological samples based on a cyclic enzymatic amplification method.

Chemical Communications, 2012
Based on graphene oxide-protected DNA probes, we have developed a cyclic enzymatic amplification method for sensitive miRNA detection in complex biological samples.
Liang Cui, Xiaoyan Lin, N. Lin, Yanling Song, Zhi Zhu, Xi Chen, C. Yang   +6 more
semanticscholar   +1 more source

[7] Probes of DNA structure

1992
Publisher Summary This chapter discusses the structure of DNA by enzymes and chemicals probing. Enzyme and chemical probing experiments have generated important insight into a number of altered DNA structures, including cruciform structures, left-handed Z-DNA, A tract-containing curved DNA, H-triplexes, G-tetrad structures, and parallel-stranded DNA,
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Binding-induced fluorescence turn-on assay using aptamer-functionalized silver nanocluster DNA probes.

Analytical Chemistry, 2012
We present here a binding-induced fluorescence turn-on assay for protein detection. Key features of this assay include affinity binding-induced DNA hybridization and fluorescence enhancement of silver nanoclusters (Ag NCs) using guanine-rich DNA ...
Jingjing Li, Xiaoqin Zhong, Hongquan Zhang, X. Le, Jun‐Jie Zhu   +4 more
semanticscholar   +1 more source

Probing RNA Translocases with DNA

2014
For some helicases, it is possible to investigate RNA translocase activity on DNA substrates because the enzyme acts on both substrates. Potassium permanganate (KMnO4) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins.
Kimberly A. Reynolds, Kevin D. Raney, Veronica M. Raney   +2 more
openaire   +3 more sources

Enzyme-Labeled DNA Probes

2003
Many research workers and clinicians working in routine laboratories are now using the "tools" developed by molecular biologists over the past 10 years to produce DNA sequences that can be used as probes to detect specific genes. Thus it is possible to screen food for salmonella (1) or other organisms in a test much more quickly than conventional ...
J. Lesley Woodhead, Rachel A. Fallon, Hermia Figueiredo, Alan D. B. Malcolm   +3 more
openaire   +3 more sources

Probing Amyloid-DNA Interaction with Nanofluidics

2022
Nanofluidics is an emerging methodology to investigate single biomacromolecules without functionalization and/or attachment of the molecules to a substrate. In conjunction with fluorescence microscopy, it can be used to investigate structural and dynamical aspects of amyloid-DNA interaction.
Basak, Rajib, Yadav, Indresh, Arluison, Véronique, van Kan, Jeroen, van der Maarel, Johan   +4 more
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Small DNA circles as probes of DNA topology

Biochemical Society Transactions, 2013
Small DNA circles can occur in Nature, for example as protein-constrained loops, and can be synthesized by a number of methods. Such small circles provide tractable systems for the study of the structure, thermodynamics and molecular dynamics of closed-circular DNA.
Anthony Maxwell, Agnes Noy, Andrew D. Bates, Michael M. Piperakis, Sarah A. Harris   +4 more
openaire   +3 more sources