Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes [PDF]
, 1984 Two cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization ...
C. R. M�ller +30 more
core +1 more source
DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications
BioTechniques, 2001 Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes.
Vladimir V. Didenko
doaj +1 more source
A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization [PDF]
, 2009 Quantum dots (Qdots) are semiconductor nanocrystals, which are photo-stable, show bright fluorescence with narrow, symmetric emission spectra and are available in multiple resolvable colors.
Cremer, M. +4 more
core +1 more source
Biosensing using hairpin DNA probes
Reviews in Analytical Chemistry, 2015 Hairpin DNA probes (HDPs) are specially designed single-stranded DNA and have excellent sensing specificity. The past decade has witnessed the fast development of HDP-based biosensors due to the tremendous applications in biology, medicine, environmental
Huang Jiahao, Wu Jueqi, Li Zhigang
doaj +1 more source
Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea) [PDF]
, 2018 Ascaridoid nematodes belonging to the genera Anisakis and Pseudoterranova are heteroxenous parasites, involving marine mammals as definitive hosts in their life-cycles, whereas crustaceans (krill), fish and squids acting as intermediate/paratenic hosts.
Colantoni, Alessandra +5 more
core +1 more source
DNA binding hydroxyl radical probes [PDF]
Radiation Physics and Chemistry, 2012 The hydroxyl radical is the primary mediator of DNA damage by the indirect effect of ionizing radiation. It is a powerful oxidizing agent produced by the radiolysis of water and is responsible for a significant fraction of the DNA damage associated with ionizing radiation.
Vicky J, Tang +3 more
openaire +2 more sources
Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR
BioTechniques, 2004 Real-time PCR technology using dual-labeled fluorescent oligonucleotide probes allows for sensitive, specific, and quantitative determination of mRNA or DNA targets.
Anthony T. Yeung +3 more
doaj +1 more source
Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
, 2013 Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
core +3 more sources
Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]
, 2016 We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto +3 more
core +2 more sources
BioTechniques, 2008 Microarrays printed on glass slides are often constructed by covalently linking modified oligonucleotide probes to a derivatized surface at considerable expense.
Haukur Gudnason +3 more
doaj +1 more source