Electrically Guided DNA Immobilization and Multiplexed DNA Detection with Nanoporous Gold Electrodes. [PDF]
Molecular diagnostics have significantly advanced the early detection of diseases, where the electrochemical sensing of biomarkers (e.g., DNA, RNA, proteins) using multiple electrode arrays (MEAs) has shown considerable promise.
Daggumati, Pallavi +3 more
core +3 more sources
Development of probes that allow for sequence-unrestricted recognition of double-stranded DNA (dsDNA) continues to attract much attention due to the prospect for molecular tools that enable detection, regulation, and manipulation of genes.
Brooke A. Anderson +2 more
doaj +1 more source
In the present study we describe sandwich design hybridization probes consisting of magnetic particles (MP) and quantum dots (QD) with target DNA, and their application in the detection of avian influenza virus (H5N1) sequences. Hybridization of 25-, 40-,
Alexey Dan Chin Yu +4 more
doaj +1 more source
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy [PDF]
Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single ...
Jalocha, A. +5 more
core +3 more sources
Physico-chemical foundations underpinning microarray and next-generation sequencing experiments [PDF]
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS).
A. Buhot +70 more
core +8 more sources
Development and application of DNA molecular probes
The development of DNA probes started from 1950's for diagnostic purposes and it is still growing. DNA probes are applied in several fields such as food, medical, veterinary, environment and security, with the aim of prevention, diagnosis and treatment ...
Priya Vizzini +3 more
doaj +1 more source
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye.
W. Gregory Cox, Victoria L. Singer
doaj +1 more source
A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones [PDF]
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ ...
A Kallioniemi +31 more
core +1 more source
DNA fingerprinting and cloning of hypervariable minisatellite repeats in salmonids [PDF]
We used heterologous jeffreys' 33.6 core sequence and microsatellites (CAC)(5) and (CA)(12) as probes and compared them with probes based on the minisatellite sequences from tilapia (Oreochromis niloticus) and Atlantic salmon (Salmo salar) in ...
Leung, FCC +3 more
core +1 more source
Multicolor chromosome bar codes [PDF]
Chromosome bar codes are multicolor banding patterns produced by fluorescence in situ hybridization (FISH) with differentially labeled and pooled sub-regional DNA probes.
Müller, Stefan, Wienberg, Johannes
core +1 more source

