Probing Rad51-DNA interactions by changing DNA twist [PDF]
Nucleic Acids Research, 2012 In eukaryotes, Rad51 protein is responsible for the recombinational repair of double-strand DNA breaks. Rad51 monomers cooperatively assemble on exonuclease-processed broken ends forming helical nucleo-protein filaments that can pair with homologous regions of sister chromatids.
Atwell, Scott +7 more
openaire +9 more sources
Label-Free Potentiometry for Detecting DNA Hybridization Using Peptide Nucleic Acid and DNA Probes
Italian National Conference on Sensors, 2013 Peptide nucleic acid (PNA) has outstanding affinity over DNA for complementary nucleic acid sequences by forming a PNA-DNA heterodimer upon hybridization via Watson-Crick base-pairing.
T. Goda +6 more
semanticscholar +1 more source
Rescue of DNA damage after constricted migration reveals a mechano-regulated threshold for cell cycle. [PDF]
, 2019 Migration through 3D constrictions can cause nuclear rupture and mislocalization of nuclear proteins, but damage to DNA remains uncertain, as does any effect on cell cycle.
Bannister +77 more
core +1 more source
Long Oligonucleotide Arrays on Nylon for Large-Scale Gene Expression Analysis
BioTechniques, 2002 To date, most studies of multigenic expression patterns by long DNA array have used DNA fragments as probes. These probes are usually obtained as PCR products, and this represents a time-consuming and error-prone approach, requiring strict quality ...
M. El Atifi +5 more
doaj +1 more source
Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes [PDF]
, 1984 Two cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization ...
C. R. M�ller +30 more
core +1 more source
Probing helical transitions in a DNA duplex [PDF]
Physical Chemistry Chemical Physics, 2017 Conformational switch from B-DNA to Z-DNA, an energy landscape perspective.
Wales, DJ, Chakraborty, Debayan
openaire +3 more sources
Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection [PDF]
, 2007 Background: Affymetrix GeneChip arrays are widely used for transcriptomic studies in a diverse range of species. Each gene is represented on a GeneChip array by a probe-set, consisting of up to 16 probe-pairs. Signal intensities across probe-pairs within
Broadley, Martin R. +4 more
core +4 more sources
Direct probing: covalent attachment of probe DNA to double-stranded target DNA [PDF]
Nucleic Acids Research, 1998 We report a novel procedure, which can be applied to probing of specific DNA, for covalently attaching probe DNA to complementary sequences in double-stranded target DNA. Employing hairpin-like oligonucleotide probes in combination with successive use of recA protein and DNA ligase, probes can be attached directly to target DNA molecules without ...
Michio Oishi, Jun Fujiwara
openaire +3 more sources
FoxO1 signaling in B cell malignancies and its therapeutic targeting
FEBS Letters, EarlyView.FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac +3 more
wiley +1 more source
DNA-based digital tension probes reveal integrin forces during early cell adhesion
Nature Communications, 2014 Mechanical stimuli profoundly alter cell fate, yet the mechanisms underlying mechanotransduction remain obscure because of a lack of methods for molecular force imaging.
Yun Zhang +3 more
semanticscholar +1 more source