Results 21 to 30 of about 566,456 (323)
Replicative DNA Polymerases [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 In 1959, Arthur Kornberg was awarded the Nobel Prize for his work on the principles by which DNA is duplicated by DNA polymerases. Since then, it has been confirmed in all branches of life that replicative DNA polymerases require a single-stranded template to build a complementary strand, but they cannot start a new DNA strand de novo.
Johansson, Erik, Dixon, Nicholas
openaire +4 more sources
Persistence length of chromatin determines origin spacing in Xenopus early-embryo DNA replication: Quantitative comparisons between theory and experiment [PDF]
Cell Cycle 3, 223-229 (2004), 2003 In Xenopus early embryos, replication origins neither require specific DNA sequences nor is there an efficient S/M checkpoint, even though the whole genome (3 billion bases) is completely duplicated within 10-20 minutes. This leads to the"random-completion problem" of DNA replication in embryos, where one needs to find a mechanism that ensures complete,
arxiv +1 more source
Quantitative sensing and signalling of single-stranded DNA during the DNA damage response
Nature Communications, 2019 DNA damage triggers checkpoint signalling mechanisms. Here the authors reveal differential phosphorylation of targets of the Mec1-Ddc2 checkpoint kinase by analyzing the effect of quantitatively different ssDNA signals.
Susanne C. S. Bantele +2 more
doaj +1 more source
Nucleation and growth in one dimension, part II: Application to DNA replication kinetics [PDF]
Phys. Rev. E 71, 011909 (2005), 2004 Inspired by recent experiments on DNA replication, we apply a one-dimensional nucleation-and-growth model to DNA-replication kinetics, focusing on how to extract the time-dependent nucleation rate I(t) and growth speed v from data. We discuss generic experimental problems, namely spatial inhomogeneity, measurement noise, and finite-size effects.
arxiv +1 more source
Error correction during DNA replication [PDF]
Physical Review E, VOL.86, 011913 (2012), 2011 DNA polymerase (DNAP) is a dual-purpose enzyme that plays two opposite roles in two different situations during DNA replication. It plays its normal role as a {\it polymerase} catalyzing the elongation of a new DNA molecule by adding a monomer. However, it can switch to the role of an {\it exonuclease} and shorten the same DNA by cleavage of the last ...
arxiv +1 more source
eLife, 2017 DNA double strand breaks (DSBs) can be repaired by either recombination-based or direct ligation-based mechanisms. Pathway choice is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repair by recombination ...
Susanne CS Bantele +4 more
doaj +1 more source
SARS-CoV-2: from its discovery to genome structure, transcription, and replication
Cell & Bioscience, 2021 SARS-CoV-2 is an extremely contagious respiratory virus causing adult atypical pneumonia COVID-19 with severe acute respiratory syndrome (SARS). SARS-CoV-2 has a single-stranded, positive-sense RNA (+RNA) genome of ~ 29.9 kb and exhibits significant ...
Ayslan Castro Brant +4 more
doaj +1 more source
Attenuation of reverse transcriptase facilitates SAMHD1 restriction of HIV-1 in cycling cells
Retrovirology, 2023 Background SAMHD1 is a deoxynucleotide triphosphohydrolase that restricts replication of HIV-1 in differentiated leucocytes. HIV-1 is not restricted in cycling cells and it has been proposed that this is due to phosphorylation of SAMHD1 at T592 in these ...
Ming-Han C. Tsai +8 more
doaj +1 more source
Molecular Docking for the Development of Alternative Therapies against Leishmaniasis
Chemistry Proceedings, 2023 Topoisomerases play a pivotal role in regulating the topological structure of DNA during fundamental processes such as transcription, DNA repair, or DNA replication; because of this, topoisomerases are biological targets in pathogenic microorganisms or ...
Juan Diego Guarimata +3 more
doaj +1 more source
Case Reports in Genetics, 2021 Mitochondrial DNA (mtDNA) depletion syndromes are a group of autosomal recessive disorders associated with a spectrum of clinical diseases, which include progressive external ophthalmoplegia (PEO).
Justin Kurtz +4 more
doaj +1 more source