SCF-FBXO31 E3 ligase targets DNA replication factor Cdt1 for proteolysis in the G2 phase of cell cycle to prevent re-replication. [PDF]
J Biol Chem, 2014 FBXO31 was originally identified as a putative tumor suppressor gene in breast, ovarian, hepatocellular, and prostate cancers. By screening a set of cell cycle-regulated proteins as potential FBXO31 interaction partners, we have now identified Cdt1 as a novel substrate.
Johansson P +6 more
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Regulation of cell cycle progression by forkhead transcription factor FOXO3 through its binding partner DNA replication factor Cdt1. [PDF]
Proc Natl Acad Sci U S A, 2012 To ensure genome stability, DNA must be replicated once and only once during each cell cycle. Cdt1 is tightly regulated to make sure that cells do not rereplicate their DNA. Multiple regulatory mechanisms operate to ensure degradation of Cdt1 in S phase. However, little is known about the positive regulators of Cdt1 under physiological conditions. Here
Zhang Y +6 more
europepmc +6 more sources
Chromatin Licensing and DNA Replication Factor 1 (CDT1) Is a Potential Prognostic Biomarker Involved in the Malignant Biological Behavior of Glioma. [PDF]
ACS Pharmacol Transl SciGlioma is the primary malignant tumor with the highest incidence rate in the adult central nervous system. The application of bioinformatics methods to analyze the RNA sequences of multiple gliomas revealed that the CDT1 gene has a significant impact on the cell cycle of glioma cells. Subsequently, we comprehensively and systematically investigated the
Chen T, Meng J, Yu K, Huang T, Zhao J.
europepmc +5 more sources
Stress-Stimulated Mitogen-Activated Protein Kinases Control the Stability and Activity of the Cdt1 DNA Replication Licensing Factor [PDF]
Molecular and Cellular Biology, 2011 DNA replication is tightly coordinated both with cell cycle cues and with responses to extracellular signals to maintain genome stability. We discovered that human Cdt1, an essential origin licensing protein whose activity must be restricted to G1 phase, is a substrate of the stress-activated mitogen-activated protein (MAP) kinases p38 and c-Jun N ...
Srikripa, Chandrasekaran +3 more
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Acetylation/Deacetylation Modulates the Stability of DNA Replication Licensing Factor Cdt1 [PDF]
Journal of Biological Chemistry, 2009 Proper expression of the replication licensing factor Cdt1 is primarily regulated post-translationally by ubiquitylation and proteasome degradation. In a screen to identify novel non-histone targets of histone deacetylases (HDACs), we found Cdt1 as a binding partner for HDAC11. Cdt1 associates specifically and directly with HDAC11.
Michele A, Glozak, Edward, Seto
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CDT1 (chromatin licensing and DNA replication factor 1) [PDF]
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011 Review on CDT1 (chromatin licensing and DNA replication factor 1), with data on DNA, on the protein encoded, and where the gene is implicated.
Deka, R, Tamuli, R
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Proteolysis of DNA Replication Licensing Factor Cdt1 in S-phase Is Performed Independently of Geminin through Its N-terminal Region [PDF]
Journal of Biological Chemistry, 2004 Licensing of replication origins is carefully regulated in a cell cycle to maintain genome integrity. Using an in vivo ubiquitination assay and temperature-sensitive cell lines we demonstrate that Cdt1 in mammalian cells is degraded through ubiquitin-dependent proteolysis in S-phase.
Hideo, Nishitani +2 more
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The Human Licensing Factor for DNA Replication Cdt1 Accumulates in G1 and Is Destabilized after Initiation of S-phase [PDF]
Journal of Biological Chemistry, 2001 S-phase onset is controlled, so that it occurs only once every cell cycle. DNA is licensed for replication after mitosis in G(1), and passage through S-phase removes the license to replicate. In fission yeast, Cdc6/18 and Cdt1, two factors required for licensing, are central to ensuring that replication occurs once per cell cycle.
H, Nishitani +3 more
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cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis. [PDF]
The EMBO Journal, 1994 We have used an immunoprecipitation-PCR cycle to isolate physically genomic DNA sequences that are bound by the fission yeast cdc10 gene product in an attempt to identify novel target genes. An essential gene, cdt1, has been isolated whose expression is cell cycle regulated in a cdc10 dependent manner.
J F, Hofmann, D, Beach
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Geminin inhibits DNA replication licensing by sterically blocking CDT1-MCM2 interactions [PDF]
Nature CommunicationsDNA replication is tightly regulated to occur once per cell cycle, with the MCM2-7 helicase loaded onto replication origins only during G1-phase. In higher eukaryotes, geminin negatively regulates this process during S-, G2- and M-phases by binding the ...
Joshua Tomkins +13 more
doaj +2 more sources