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Structural Insights into Eukaryotic DNA Replication [PDF]
Frontiers in Microbiology, 2014 Three DNA polymerases of the B family function at the replication fork in eukaryotic cells: DNA polymerases α, δ, and ε. DNA polymerase α, an heterotetramer composed of two primase subunits and two polymerase subunits, initiates replication.
Sylvie eDoublié, Karl E Zahn
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Eukaryotic DNA Replication Fork. [PDF]
Annu Rev Biochem, 2017 This review focuses on the biogenesis and composition of the eukaryotic DNA replication fork, with an emphasis on the enzymes that synthesize DNA and repair discontinuities on the lagging strand of the replication fork. Physical and genetic methodologies aimed at understanding these processes are discussed.
Burgers PMJ, Kunkel TA.
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Archaeology of eukaryotic DNA replication. [PDF]
Cold Spring Harb Perspect Biol, 2013 Recent advances in the characterization of the archaeal DNA replication system together with comparative genomic analysis have led to the identification of several previously uncharacterized archaeal proteins involved in replication and currently reveal a nearly complete correspondence between the components of the archaeal and eukaryotic replication ...
Makarova KS, Koonin EV.
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Emerging players in the initiation of eukaryotic DNA replication [PDF]
Cell Division, 2012 Faithful duplication of the genome in eukaryotes requires ordered assembly of a multi-protein complex called the pre-replicative complex (pre-RC) prior to S phase; transition to the pre-initiation complex (pre-IC) at the beginning of DNA replication ...
Shen Zhen, Prasanth Supriya G
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Nucleosome-directed replication origin licensing independent of a consensus DNA sequence
Nature Communications, 2022 Most eukaryotes do not use a consensus DNA sequence as binding sites for the origin recognition complex (ORC) to initiate DNA replication, however budding yeast do. Here the authors show S. cerevisiae ORC can bind nucleosomes near nucleosome-free regions
Sai Li +5 more
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Research, 2022 DNA replication initiation is a complex process involving various genetic and epigenomic signatures. The correct identification of replication origins (ORIs) could provide important clues for the study of a variety of diseases caused by replication. Here,
Fu-Ying Dao +3 more
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Nature Communications, 2021 Eukaryotic DNA replication is mediated by many proteins which are tightly regulated for an efficient firing of replication at each cell cycle. Here the authors report a cryo-EM structure of the yeast ORC–Cdc6 bound to an 85-bp ARS1 origin DNA revealing ...
Xiang Feng +5 more
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The organizer of chromatin topology RIF1 ensures cellular resilience to DNA replication stress
Life Science Alliance, 2023 The conserved protein RIF1 stabilizes replication factories and regulates cohesin recruitment in response to replication stress to limit DNA lesions. Eukaryotic genomes are duplicated from thousands of replication origins that fire sequentially forming a
Rana Lebdy +6 more
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Cell Reports, 2020 Summary: Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle.
Aftab Amin +10 more
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Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy [PDF]
, 2014 Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms.
Adam T. Watson +46 more
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