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Restriction enzyme analysis of the Plasmid ColIb DNA
Molecular and General Genetics MGG, 1979Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated.
A, Skorupska +2 more
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Restriction enzyme analysis of human leukemic mitochondrial DNA
Leukemia Research, 1980Abstract Mitochondrial DNA from both normal human tissue and leukemic human leukocytes (AML and CML) were analyzed by restriction-enzyme digestion, polyacrylamide gradient gel electrophoresis and ethidium bromide staining. Both normal and leukemic human mitochondrial DNA show molecular heterogeneity from individual to individual.
A.M. Gianni, R. Dalla Favera, E. Polli
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Restriction enzyme studies on human highly repeated DNAs
Experientia, 1982Various restriction enzymes digest human highly repeated homogeneous DNA to discrete fragments, some of which are present in the male and absent in the female. The male specific 2.4 kilobase HaeIII fragment corresponds to human male satellite DNA IV.
G. Corneo +4 more
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Recognition of DNA by Type II Restriction Enzymes
1989Publisher Summary This chapter discusses the recognition of DNA by type II restriction enzymes. A restriction/modification (R/M) system must possess two enzyme activities, the restriction endonuclease and the modification methylase, both of which are dependent on the recognition of the same DNA sequence.
S P, Bennett, S E, Halford
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Physical map of bacteriophage BF23 DNA: restriction enzyme analysis
Journal of Virology, 1979Cleavage maps of bacteriophage BF23 DNA have been constructed for the restriction endonucleases SalI (3 fragments), BamHI (5 fragments), EcoRI, (8 fragments), BalI (13 fragments), and HpaI (49 fragments, 32 of which have been ordered). The maps were determined by (i) analysis of deletion mutants, (ii) digestion with two endonucleases, (iii) digestion ...
B, Lange-Gustafson, M, Rhoades
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DNA fingerprinting of yeast strains by restriction enzyme analysis
Research in Microbiology, 1994Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained.
BARBERIO, CLAUDIA +4 more
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Resistance of Tumor-Derived DNA to Restriction Enzyme Digestion
Cancer Investigation, 1990The major finding of this work is that there are specific restriction enzyme inhibitors present in "purified" tumor DNA which cause partial digestion patterns when tumor DNA is digested by standard procedures with any of three commonly employed restriction enzymes (HindIII, KpnI, XbaI).
J L, Parkes, F C, Hubbard, A, Penn
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Restriction enzyme analysis of mitochondrial DNA in colorectal tumours
Biochemical and Biophysical Research Communications, 1991Total cellular DNA samples were isolated from 15 colorectal adenocarcinomas, 8 colon adenomas and their adjacent histologically normal colon mucosa. These DNA samples were digested separately with 13 different restriction endonucleases and analysed by Southern blot hybridization using a purified 32P-labelled human mtDNA probe.
W, Cui, I C, Talbot, J M, Northover
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1992
An approach to high-resolution restriction-fragment DNA mapping, known as Multiple-Restriction-Enzyme mapping (MRE mapping), is present. This approach significantly reduces the uncertainty of clone placement by using clone ends to synchronize the position in of clones within different maps, each map being constructed from fragment-length data produced ...
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An approach to high-resolution restriction-fragment DNA mapping, known as Multiple-Restriction-Enzyme mapping (MRE mapping), is present. This approach significantly reduces the uncertainty of clone placement by using clone ends to synchronize the position in of clones within different maps, each map being constructed from fragment-length data produced ...
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Exploring Ligand-DNA Space Using Type IIS Restriction Enzymes
Combinatorial Chemistry & High Throughput Screening, 2002Investigating ligand-DNA interactions using type IIS restriction enzymes (IISRE) as footprinting reagents is reviewed and contemplated. Ligand binding at a IISRE's cleavage but not sequence recognition site protects DNA from strand scission. This spatial arrangement has been exploited in the development of qualitative (combinatorial) and quantitative ...
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