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Biomolecular computers with multiple restriction enzymes [PDF]

open access: yesGenetics and Molecular Biology, 2017
The development of conventional, silicon-based computers has several limitations, including some related to the Heisenberg uncertainty principle and the von Neumann “bottleneck”.
Sebastian Sakowski   +5 more
doaj   +4 more sources

Type I restriction enzymes and their relatives [PDF]

open access: yesNucleic Acids Research, 2014
Type I restriction enzymes (REases) are large pentameric proteins with separate restriction (R), methylation (M) and DNA sequence-recognition (S) subunits. They were the first REases to be discovered and purified, but unlike the enormously useful Type II
Elisabeth A Raleigh   +2 more
exaly   +4 more sources

Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]

open access: yesPLoS ONE, 2014
The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth   +8 more
doaj   +3 more sources

Restriction Enzymes [PDF]

open access: yesCold Spring Harbor Protocols, 2021
Restriction enzymes provided the foundation on which molecular cloning was built, and they remain as essential tools in current recombinant DNA technology. The three classes of restriction enzymes and their features are introduced here.
Michael R, Green, Joseph, Sambrook
openaire   +3 more sources

Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

open access: yesFermentation, 2023
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj   +1 more source

REBASE--restriction enzymes and methylases [PDF]

open access: yesNucleic Acids Research, 1993
REBASE is a comprehensive database of information about restriction enzymes and related proteins. It contains published and unpublished references, recognition and cleavage sites, isoschizomers, commercial availability, methylation sensitivity, crystal and sequence data.
Richard J. Roberts, Dana Macelis
openaire   +18 more sources

Highlights of the DNA cutters: a short history of the restriction enzymes [PDF]

open access: yesNucleic Acids Research, 2014
Elisabeth A Raleigh   +2 more
exaly   +2 more sources

The Effect of Myostatin Gene Polymorphisms on Some Biochemist Traits for Blood in Broiler Chicken [PDF]

open access: yesAl-Qadisiyah Journal For Agriculture Sciences, 2017
This study is conducted at the Poultry Farm of Animal Production Department of College of Agriculture, University of Al-Qadisiyah during the period 9/4/2015 - 20/5/2015 and in a laboratory of molecular genetic analysis of the College of Agriculture ...
Adnan Hussein Mohammed   +1 more
doaj   +1 more source

Isolation, Purification and Crystallization of Restriction Enzyme Bsa I and Its Preparation of Seleno-derived Derivatives

open access: yesShipin gongye ke-ji, 2023
Objective: To screen and optimize the method for preparing three-dimensional structural samples of restriction endonuclease Bsa I. Methods: In this study, the Escherichia coli expression system was employed to express the Bsa I protein and its ...
Hai ZHU   +8 more
doaj   +1 more source

REHUNT: a reliable and open source package for restriction enzyme hunting

open access: yesBMC Bioinformatics, 2018
Background Restriction enzymes are used frequently in biotechnology. However, manual mining of restriction enzymes is challenging. Furthermore, integrating available restriction enzymes into different bioinformatics systems is necessary for many ...
Yu-Huei Cheng   +2 more
doaj   +1 more source

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