Results 21 to 30 of about 1,137,892 (286)

A CRISPR-based approach using dead Cas9-sgRNA to detect SARS-CoV-2

Frontiers in Molecular Biosciences, 2023
Rapid, highly specific, and robust diagnostic kits to detect viruses and pathogens are needed to control disease spread and transmission globally. Of the many different methods proposed to diagnose COVID-19 infection, CRISPR-based detection of nucleic ...
Mustapha Aouida, Maryam Saifaldeen, Dana E. Al-Ansari, Dana E. Al-Ansari, Sara Taleb, Ali Ait Hssain, Ali Ait Hssain, Ali Ait Hssain, Dindial Ramotar   +8 more
doaj   +1 more source

Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes [PDF]

, 2009
Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used
Neely, Robert K., Jones, Anita C., Tamulaitis, Gintautas, Kubala, Marta, Neely, Robert K; id_orcid, Chen, Kai, Siksnys, Virginijus, Jones, Anita C   +7 more
core   +1 more source

Using of Data Base to Determine the Restriction Sites and Drawing Restriction Map for Lipase Gene from Bacillus stearothermophilus

مجلة مركز بحوث التقنيات الاحيائية, 2011
pecific data base was used for restriction enzymes (rebase) and related proteins, to design executive program in quick basic language to determine the restriction sites and drawing restriction map for the complete sequence of lipase gene from Bacillus ...
Hameed M. Jasim
doaj   +1 more source

REtools: A laboratory program for restriction enzyme work: enzyme selection and reaction condition assistance

BMC Bioinformatics, 2006
Background Restriction enzymes are one of the everyday tools used in molecular biology. The continuously expanding panel of known restriction enzymes (several thousands) renders their optimal use virtually impossible without computerized assistance ...
Boulukos Kim E, Martin Patrick, Pognonec Philippe   +2 more
doaj   +1 more source

Putative RFLP Analysis Between HSVd-sycv and Closely Related Variants [PDF]

Journal of Cell and Molecular Research, 2016
Yellow corky vein is a prevalent disease among navel oranges in the Fars province of Iran. Previously we showed that a variant of Hop stunt viroid (HSVd-sycv) was associated with the disease.
seyed ali akbar Bagherian
doaj   +1 more source

A NOVEL APPROACH TO GENOTYPING OF HOSPITAL ISOLATES OF CLOSTRIDIUM DIFFICILE

Журнал микробиологии, эпидемиологии и иммунобиологии, 2016
Aim. Development of a novel approach in genotyping of Clostridium difficile and its testing on the example of 140 hospital isolates. Materials and methods.
V. P. Terletsky, V. I. Tyschenko, O. B. Novikova, I. I. Novikova, E. D. Dzhavadov   +4 more
doaj   +1 more source

The restriction profile of the transformed phage ADUt with different restriction enzymes.

, 2023
A) The expected band size of ADUt phage with different restriction enzymes was obtained by in silico analysis of ADUt phage genome. B) The restriction profile of ADUt phage with different restriction enzymes was obtained by in vitro analysis of isolated ...
Bülent Bozdoğan (17145044), Abdulkerim Karaynir (17145041), Hanife Salih Doğan (17145047)   +2 more
core   +1 more source

Restriction site heteroplasmy in the mitochondrial DNA of Brycon opalinus (Cuvier, 1819) (Characiformes, Characidae, Bryconiae)

Brazilian Journal of Medical and Biological Research, 2004
Homoplasmy is a feature usually found in the mtDNA of higher animal taxa. On the other hand, the presence of two classes of mtDNA in the same cell or organism is rare and may appear in length or site variation.
A.W.S. Hilsdorf, J.E. Krieger
doaj   +1 more source

A simple method for the genetic identification of commercially important species in the Opisthonema genus Gill, 1861 in the southern Gulf of California

Ciencias Marinas, 2020
The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to identify 3 species in the genus Opisthonema (Opisthonema bulleri, Opisthonema medirastre, and Opisthonema libertate) in the southern Gulf of ...
Carlos Iván Pérez-Quiñonez, Casimiro Quiñonez-Velázquez, Francisco Javier García-Rodríguez   +2 more
doaj   +1 more source

Genotyping by Sequencing Advancements in Barley

Frontiers in Plant Science, 2022
Barley is considered an ideal crop to study cereal genetics due to its close relationship with wheat and diploid ancestral genome. It plays a crucial role in reducing risks to global food security posed by climate change. Genetic variations in the traits
Nirmal Raj Rajendran, Naeela Qureshi, Mohammad Pourkheirandish   +2 more
doaj   +1 more source