Results 301 to 310 of about 390,387 (330)
Inhibition of DNA restriction enzyme digestion by anthracyclines [PDF]
La Ricerca in Clinica e in Laboratorio, 1988 The inhibition of restriction enzyme digestion of lambda phage DNA by anthracyclines (i.e., adriamycin, daunomycin, epirubicin, idarubicin and esorubicin) commonly used in the treatment of human leukemia and cancer has been studied in vitro. The anthracyclines used inhibit DNA digestion by SmaI, AvaII, HaeIII, HhaI and HpaII, which cut DNA at guanine ...
G. Corneo +3 more
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DNA Restriction Enzyme from E. coli [PDF]
Nature, 1968 An endonuclease which degrades foreign DNA has been isolated. The enzyme requires S-adenosylmethionine, ATP and Mg++.
Robert Yuan, Matthew Meselson
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Restriction enzyme analysis of the Plasmid ColIb DNA
Molecular and General Genetics MGG, 1979Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated.
Anna Skorupska +2 more
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Restriction enzyme cleavage of ultraviolet-damaged DNA
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1982SV40 and pBR322 DNAs damaged by ultraviolet light were cleaved abnormally by several restriction enzymes because of damage to pyrimidines in the recognition sequences. The use of a tandemly duplicated plasmid provided a particularly sensitive target molecule for detecting pyrimidine dimers and other possible photoproducts.
James E. Cleaver, L. Samson, G.H. Thomas
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1987
The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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The discovery of restriction and modification enzymes, which proved to be a major turning point in the progress of molecular biology, was a consequence of a bacteriological observation in the early 1950s (Luria and Human, 1952; Bertani and Weigle, 1953).
Alan D. B. Malcolm, Georges Snounou
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Programmable DNA-Guided Artificial Restriction Enzymes
ACS Synthetic Biology, 2017Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research. However, they have limited sequence specificity and availability. Here we report a Pyrococcus furiosus Argonaute (PfAgo) based platform for generating artificial restriction enzymes (AREs) capable of recognizing and cleaving DNA ...
Behnam Enghiad, Huimin Zhao
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Restriction Enzyme-Mediated DNA Family Shuffling
2014DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H. +2 more
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Recognition of DNA by Type II Restriction Enzymes
1989Publisher Summary This chapter discusses the recognition of DNA by type II restriction enzymes. A restriction/modification (R/M) system must possess two enzyme activities, the restriction endonuclease and the modification methylase, both of which are dependent on the recognition of the same DNA sequence.
Bennett Sp, Halford Se
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Restriction enzyme studies on human highly repeated DNAs
Experientia, 1982Various restriction enzymes digest human highly repeated homogeneous DNA to discrete fragments, some of which are present in the male and absent in the female. The male specific 2.4 kilobase HaeIII fragment corresponds to human male satellite DNA IV.
G. Corneo +4 more
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