Results 301 to 310 of about 634,910 (347)

Recognition of DNA by Type II Restriction Enzymes

1989
Publisher Summary This chapter discusses the recognition of DNA by type II restriction enzymes. A restriction/modification (R/M) system must possess two enzyme activities, the restriction endonuclease and the modification methylase, both of which are dependent on the recognition of the same DNA sequence.
S P, Bennett, S E, Halford
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Restriction enzyme analysis of human leukemic mitochondrial DNA

Leukemia Research, 1980
Abstract Mitochondrial DNA from both normal human tissue and leukemic human leukocytes (AML and CML) were analyzed by restriction-enzyme digestion, polyacrylamide gradient gel electrophoresis and ethidium bromide staining. Both normal and leukemic human mitochondrial DNA show molecular heterogeneity from individual to individual.
A.M. Gianni, R. Dalla Favera, E. Polli
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Restriction Enzyme-Mediated DNA Family Shuffling

2014
DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without ...
Behrendorff, James B.Y.H., Johnston, Wayne A., Gillam, Elizabeth M.J.   +2 more
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DNA fingerprinting of yeast strains by restriction enzyme analysis

Research in Microbiology, 1994
Restriction endonuclease analysis was used as a new method to obtain genomic DNA fingerprints in yeast. Fifteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces were examined. Restriction fragments obtained with ApaI or KspI endonucleases were separated by SDS-PAGE and silver-stained.
BARBERIO, CLAUDIA, FANI, RENATO, A. Raso, A. Carli, POLSINELLI, MARIO   +4 more
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Resistance of Tumor-Derived DNA to Restriction Enzyme Digestion

Cancer Investigation, 1990
The major finding of this work is that there are specific restriction enzyme inhibitors present in "purified" tumor DNA which cause partial digestion patterns when tumor DNA is digested by standard procedures with any of three commonly employed restriction enzymes (HindIII, KpnI, XbaI).
J L, Parkes, F C, Hubbard, A, Penn
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Restriction enzyme studies on human highly repeated DNAs

Experientia, 1982
Various restriction enzymes digest human highly repeated homogeneous DNA to discrete fragments, some of which are present in the male and absent in the female. The male specific 2.4 kilobase HaeIII fragment corresponds to human male satellite DNA IV.
G. Corneo, D. Meazza, M. Bregni, P. Tripputi, L. Ceccherini Nelli   +4 more
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Restriction enzyme analysis of mitochondrial DNA in colorectal tumours

Biochemical and Biophysical Research Communications, 1991
Total cellular DNA samples were isolated from 15 colorectal adenocarcinomas, 8 colon adenomas and their adjacent histologically normal colon mucosa. These DNA samples were digested separately with 13 different restriction endonucleases and analysed by Southern blot hybridization using a purified 32P-labelled human mtDNA probe.
W, Cui, I C, Talbot, J M, Northover
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The manipulation of DNA with restriction enzymes in low water systems

Biochimica et Biophysica Acta (BBA) - General Subjects, 1991
The cleavage of phage lambda (lambda) DNA by the restriction enzyme HindIII in low water systems has been investigated. Two types of low water systems have been studied--those which contain a surfactant in a reverse micelle environment and a surfactant-free system in which a solid support (celite) is used.
A B, Hanley, C S, Furniss, C A, Kwiatkowska, A R, Mackie   +3 more
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XcmI as a universal restriction enzyme for single-stranded DNA

Gene, 1993
Single-stranded DNA can be cleaved into defined fragments at any predetermined site by interaction with a specially designed oligodeoxyribonucleotide (oligo) adaptor and the class-IIN restriction endonuclease, XcmI. The oligo adaptor has the structure [sequence: see text]. Upon hybridization to the target DNA through the central 9-nucleotide region and
P C, Shaw, Y K, Mok
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An assay for DNA photolyases using non-radioactive DNA and restriction enzymes

Journal of Photochemistry and Photobiology B: Biology, 1993
Restriction enzymes, such as Eco RI, Hind III, etc., which have a potential pyrimidine dimer site in their recognition sequence, fail to cleave DNA if their recognition site is modified by the formation of pyrimidine dimers as a result of UV irradiation of DNA (J. E. Cleaver, J. Mol. Biol., 170 (1983) 305-317).
K, Dutta, V S, Hejmadi, N C, Verma
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