Results 81 to 90 of about 673,449 (404)
There were diffferent performance between silkworms breeded in Soppeng and Temanggung. There might be different proportion of parenstock and environment which causing polymorphism. This experiment was to know the genetic band pattern as the effect of the
Nur Cholis
doaj +1 more source
Pulsed-Field Gel Electrophoresis Analysis of Bovine Associated Staphylococcus aureus: A Review
For decades now, DNA fingerprinting by means of pulsed-field gel electrophoresis (PFGE) continues to be the most widely used to separate large DNA molecules and distinguish between different strains in alternating pulses. This is done by isolating intact
Zoubida Dendani Chadi+1 more
doaj +1 more source
Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases
The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher+6 more
wiley +1 more source
RestrictionDigest: A powerful Perl module for simulating genomic restriction digests
Background: Reduced-representation sequencing technology is widely used in genotyping for its economical and efficient features. A popular way to construct the reduced-representation sequencing libraries is to digest the genomic DNA with restriction ...
Jinpeng Wang+4 more
doaj +1 more source
A cleavage map of bacteriophage Phi-X174 genome [PDF]
Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments.
Lee, Amy Shiu, Sinsheimer, Robert L.
core
Restriction Enzyme Analysis of Tomato Chloroplast and Chromoplast DNA [PDF]
Plastid DNA was isolated from the chloroplasts of tomato (Lycopersicon esculentum var Traveler 76) leaves and the chromoplasts of ripe tomato fruit. Comparisons of the two DNAs were made by restriction endonuclease analysis using PvuII, HpaI, and Bg1I.
Charles D. Boyer+2 more
openaire +3 more sources
LHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Victor Zegarra+7 more
wiley +1 more source
On the DNA cleavage mechanism of Type I restriction enzymes [PDF]
Although the DNA cleavage mechanism of Type I restriction-modification enzymes has been extensively studied, the mode of cleavage remains elusive. In this work, DNA ends produced by EcoKI, EcoAI and EcoR124I, members of the Type IA, IB and IC families ...
Bickle, Thomas A.+4 more
core
Restriction enzyme-mediated DNA integration in Coprinus cinereus
Restriction enzyme-mediated DNA integration (REMI) has recently received attention as a new technique for the generation of mutants by transformation in fungi. Here we analyse this method in the basidiomycete Coprinus cinereus using the homologous pabI gene as a selectable marker and the restriction enzymes BamHI, EcoRI and PstI.
Granado, J.D.+3 more
openaire +4 more sources
Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga+6 more
wiley +1 more source