Results 81 to 90 of about 391,854 (357)

Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]

PLoS ONE, 2014
The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth, Krisztina Huszár, Petra Bencsura, Péter István Kulcsár, Barbara Vodicska, Antal Nyeste, Zsombor Welker, Szilvia Tóth, Ervin Welker   +8 more
doaj   +1 more source

Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin [PDF]

, 2007
Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat ...
Chai, Lay Ching, Cheah, Yoke Kqueen, Gunsalam, Jurin Wolmon, Mat Issa @ Zakaria, Zuraini, Mohamad Ghazali, Farinazleen, Ong, Sang Bing, Radu, Son, Robin, Tunung, Yuli, Haryani   +8 more
core  

Sirtuin functions and modulation: from chemistry to the clinic [PDF]

, 2016
Sirtuins are NAD+ -dependent histone deacetylases regulating important metabolic pathways in prokaryotes and eukaryotes and are involved in many biological processes such as cell survival, senescence, proliferation, apoptosis, DNA repair, cell ...
Altucci, Lucia, Carafa, Vincenzo, Cuomo, Francesca, Forgione, Mariantonietta, Hailu, GEBREMEDHIN SOLOMON, Jarho, Elina, Lahtela Kakkonen, Maija, Mai, Antonello, Rotili, Dante, Serretiello, Enrica   +9 more
core   +2 more sources

A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes [PDF]

Nucleic Acids Research, 2003
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes.
Roberts, R. J., Belfort, M., Bestor, T., Bhagwat, A. S., Bickle, T. A., Bitinaite, J., Blumenthal, R. M., Degtyarev, S. K., Dryden, D. T. F., Dybvig, K., Firman, K., Gromova, E. S., Gumports, R. I., Halford, S. E., Hattman, S., Heitman, J., Hornby, D. P., Janulaitis, A., Jeltsch, A., Josephsen, J., Kiss, A., Klaenhammer, T. R., Kobayashi, I., Kong, H., Krüger, D. H., Lacks, S., Marinus, M. G., Miyahara, M., Morgan, R. D., Murray, N. E., Nagaraja, V., Piekarowicz, A., Pingoud, A., Raleich, E., Rao, D. N., Reich, N., Repin, V. E., Selker, E. U., Shaw, P.-C., Stein, D. C., Stoddard, B. L., Szybalski, W., Trautner, T. A., Etten, J. L. v., Vitor, J. M. B., Wilson, G. G., Xu, S.-Y.   +46 more
openaire   +8 more sources

Neutrophil deficiency increases T cell numbers at the site of tissue injury in mice

FEBS Letters, EarlyView.
In wild‐type mice, injury or acute inflammation induces neutrophil influx followed by macrophage accumulation. Mcl1ΔMyelo (neutrophil‐deficient) mice lack neutrophils, and in response to muscle injury show fewer macrophages and exhibit strikingly elevated T‐cell numbers, primarily non‐conventional “double‐negative” (DN) αβ and γδ T cells.
Hajnalka Halász, Albert Bálint Papp, Lukács Sándor Lesinszki, Attila Mócsai, Tamás Varga, László Nagy, Péter Gogolák   +6 more
wiley   +1 more source

Restriction enzymes use a 24 dimensional coding space to recognize 6 base long DNA sequences.

PLoS ONE, 2019
Restriction enzymes recognize and bind to specific sequences on invading bacteriophage DNA. Like a key in a lock, these proteins require many contacts to specify the correct DNA sequence.
Thomas D Schneider, Vishnu Jejjala
doaj   +1 more source

The Epigenomic Landscape of Prokaryotes. [PDF]

PLoS Genetics, 2016
DNA methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. Studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and ...
Matthew J Blow, Tyson A Clark, Chris G Daum, Adam M Deutschbauer, Alexey Fomenkov, Roxanne Fries, Jeff Froula, Dongwan D Kang, Rex R Malmstrom, Richard D Morgan, Janos Posfai, Kanwar Singh, Axel Visel, Kelly Wetmore, Zhiying Zhao, Edward M Rubin, Jonas Korlach, Len A Pennacchio, Richard J Roberts   +18 more
doaj   +1 more source

DNA restriction enzyme analysis of bovine adenoviruses.

Intervirology, 1988
Eight reference strains of the nine accepted bovine adenovirus serotypes were compared by means of restriction enzyme (RE) analysis of the viral DNA. The study was carried out with the aim to identify RE giving restriction patterns which are particularly feasible for identification of new isolates and useful for their classification into subgroups. The
Mária Benko, Göran Wadell, A. Bartha
openaire   +3 more sources

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

Restriction modification systems in archaea: A panoramic outlook

Heliyon
Restriction modification (RM) systems are one of the ubiquitous yet primitive defense responses employed by bacteria and archaea with the primary role of safeguarding themselves against invading bacteriophages.
Pallavi Gulati, Ashish Singh, Sandeep Patra, Shreyas Bhat, Anil Verma   +4 more
doaj   +1 more source