Results 81 to 90 of about 405,469 (354)

Meat molecular detection: sensitivity of polymerase chain reaction-restriction fragment length polymorphism in species differentiation of meat from animal origin [PDF]

, 2007
Three restriction enzymes were used in Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using the mitochondrial cytochrome b region to establish a differential diagnosis which detect and discriminate between three meat ...
Chai, Lay Ching, Cheah, Yoke Kqueen, Gunsalam, Jurin Wolmon, Mat Issa @ Zakaria, Zuraini, Mohamad Ghazali, Farinazleen, Ong, Sang Bing, Radu, Son, Robin, Tunung, Yuli, Haryani   +8 more
core  

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source

Genetic Analysis Using Partial Sequencing of Melanocortin 4 Receptor (MC4R) Gene in Bligon Goat

Media Peternakan, 2017
Melanocortin 4 Receptor gene is involved in sympathetic nerve activity, adrenal and thyroid functions, and media for leptin in regulating energy balance and homeostasis. The aim of this research was to perform genetic analysis of MC4R gene sequences from
Latifah Latifah, D. A. Priyadi, D. Maharani, Kustantinah Kustantinah, T. Hartatik   +4 more
doaj   +1 more source

The Epigenomic Landscape of Prokaryotes. [PDF]

PLoS Genetics, 2016
DNA methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. Studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and ...
Matthew J Blow, Tyson A Clark, Chris G Daum, Adam M Deutschbauer, Alexey Fomenkov, Roxanne Fries, Jeff Froula, Dongwan D Kang, Rex R Malmstrom, Richard D Morgan, Janos Posfai, Kanwar Singh, Axel Visel, Kelly Wetmore, Zhiying Zhao, Edward M Rubin, Jonas Korlach, Len A Pennacchio, Richard J Roberts   +18 more
doaj   +1 more source

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]

PLoS ONE, 2014
The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth, Krisztina Huszár, Petra Bencsura, Péter István Kulcsár, Barbara Vodicska, Antal Nyeste, Zsombor Welker, Szilvia Tóth, Ervin Welker   +8 more
doaj   +1 more source

Inhibition of restriction enzyme's DNA sequence recognition by PUVA treatment [PDF]

Nucleic Acids Symposium Series, 2001
Applying various restriction enzymes on a specially designed 1.5 kb DNA fragment revealed that the inhibitory effects of PUVA treatment on restriction endonuclease activities are caused by recognition inhibition. In this study, Restriction enzymes which have a 5'-TpA sequence at the cleaving site (Kpn I, Xba I, Pme I, and Dra I), and non-cleaving site (
Hanawa, F., Okamoto, M., Towers, G.
openaire   +4 more sources

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source

Restriction modification systems in archaea: A panoramic outlook

Heliyon
Restriction modification (RM) systems are one of the ubiquitous yet primitive defense responses employed by bacteria and archaea with the primary role of safeguarding themselves against invading bacteriophages.
Pallavi Gulati, Ashish Singh, Sandeep Patra, Shreyas Bhat, Anil Verma   +4 more
doaj   +1 more source

Restriction and modification of deoxyarchaeosine (dG+)-containing phage 9 g DNA

Scientific Reports, 2017
E. coli phage 9 g contains the modified base deoxyarchaeosine (dG+) in its genome. The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to synthesize and incorporate dG+.
Rebecca Tsai, Ivan R. Corrêa, Michael Y. Xu, Shuang-yong Xu   +3 more
doaj   +1 more source