Results 81 to 90 of about 390,387 (330)
Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning. [PDF]
PLoS ONE, 2014 The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert.
Eszter Tóth +8 more
doaj +1 more source
DNA restriction enzyme analysis of bovine adenoviruses.
Intervirology, 1988 Eight reference strains of the nine accepted bovine adenovirus serotypes were compared by means of restriction enzyme (RE) analysis of the viral DNA. The study was carried out with the aim to identify RE giving restriction patterns which are particularly feasible for identification of new isolates and useful for their classification into subgroups. The
Mária Benko, Göran Wadell, A. Bartha
openaire +3 more sources
Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
Restriction and modification of deoxyarchaeosine (dG+)-containing phage 9 g DNA
Scientific Reports, 2017 E. coli phage 9 g contains the modified base deoxyarchaeosine (dG+) in its genome. The phage encodes its own primase, DNA ligase, DNA polymerase, and enzymes necessary to synthesize and incorporate dG+.
Rebecca Tsai +3 more
doaj +1 more source
Investigation of DNA-protein Sequence-Specific Interactions with a ds-DNA Array
Molecules, 2005 The sequence specific recognitions between DNAs and proteins play important roles in many biological functions. The use of double-stranded DNA arrays (ds-DNA arrays) for studying sequence specific recognition between DNAs and proteins is a promising ...
Zuhong Lu +5 more
doaj +1 more source
Biochemical and mutational analysis of EcoRII functional domains reveals evolutionary links between restriction enzymes [PDF]
The archetypal Type IIE restriction endonuclease EcoRII is a dimer that has a modular structure. DNA binding studies indicate that the isolated C-terminal domain dimer has an interface that binds a single cognate DNA molecule whereas the N-terminal ...
Mucke, Merlind +2 more
core +1 more source
Archaeal diversity in deep-sea sediments estimated by means of different Terminal-Restriction Fragment Length Polymorphisms (T-RFLP) protocols [PDF]
, 2009 Despite the increasing recognition of the quantitative importance of Archaea in all marine systems, the protocols for a rapid estimate of Archaeal diversity patterns in deep-sea sediments have been only poorly tested yet.
Danovaro, Roberto +3 more
core +1 more source
Molecular Oncology, EarlyView.Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever +1 more
wiley +1 more source
BMC Biotechnology, 2005 Background Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize ...
Silverman Mel +2 more
doaj +1 more source
Characterization of the DNA of Canine Adenovirus by Restriction Enzyme Analysis
Intervirology, 1985 The DNA of canine adenovirus (canAV 1, strain Behring H.c.c. 269) was characterized in detail. The molecular weight of canAV DNA was (20.1 +/- 0.1) x 10(6) daltons as determined by contour length measurements, and 19.9 x 10(6) daltons as determined by restriction enzyme analysis.
Angela Rösen +3 more
openaire +3 more sources