Results 211 to 220 of about 1,646,380 (263)
Some of the next articles are maybe not open access.
DNA Sequencing with Transposons
Journal of Computational Biology, 2000The use of transposons offers the possibility of a directed approach to DNA sequencing, where a target DNA up to about 6kb in length can be sequenced quickly and with minimal redundancy. Transposons are mobile DNA elements which can be inserted in a reasonably random fashion into the target DNA.
Simon Cawley, Terence P. Speed
openaire +2 more sources
Aligning a DNA sequence with a protein sequence
Proceedings of the first annual international conference on Computational molecular biology - RECOMB '97, 1997We develop several algorithms for the problem of aligning DNA sequence with a protein sequence. Our methods account for frameshift errors, but not for introns in the DNA sequence. Thus, they are particularly appropriate for comparing a cDNA sequence that suffers from sequencing errors with an amino acid sequence or a protein sequence database.
Zheng Zhang 0004 +2 more
openaire +2 more sources
Characteristic Sequences for DNA Primary Sequence
Journal of Chemical Information and Computer Sciences, 2002A DNA sequence can be identified with a word over an alphabet N = [A, C, G, T]. Characteristic sequences of a DNA sequence are given in term of classifications of bases of nucleic acids. Using the characteristic sequences, we construct a set of 2 x 2 matrices to represent DNA primary sequences, which are based on counting of the frequency of occurrence
Ping-an He 0001, Jun Wang
openaire +2 more sources
1988
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
M A, Kashdan, G L, Trainor
openaire +2 more sources
DNA sequencing is a technique that is routinely performed in many molecular biology laboratories. This paper will describe some of the advances in DNA sequencing spanning the past ten years. With current manual sequencing techniques, it is possible for an average researcher to sequence 10100,000 bases per year.
M A, Kashdan, G L, Trainor
openaire +2 more sources
Science, 1988
The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
G M, Church, S, Kieffer-Higgins
openaire +2 more sources
The increasing demand for DNA sequences can be met by replacement of each DNA sample in a device with a mixture of N samples so that the normal throughput is increased by a factor of N . Such a method is described.
G M, Church, S, Kieffer-Higgins
openaire +2 more sources
2009
The ability to sequence the DNA of an organism has become one of the most important tools in modern biological research. Beginning as a manual process, where DNA was sequenced a few tens or hundreds of nucleotides at a time, DNA sequencing is now performed by high throughput sequencing machines, with billions of bases of DNA being sequenced daily ...
Edwards, D., Hansen, D., Stajich, J.E.
openaire +3 more sources
The ability to sequence the DNA of an organism has become one of the most important tools in modern biological research. Beginning as a manual process, where DNA was sequenced a few tens or hundreds of nucleotides at a time, DNA sequencing is now performed by high throughput sequencing machines, with billions of bases of DNA being sequenced daily ...
Edwards, D., Hansen, D., Stajich, J.E.
openaire +3 more sources
Current Protocols in Molecular Biology, 2006
AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
Gregory J, Porreca +2 more
openaire +2 more sources
AbstractPolony DNA sequencing provides an inexpensive, accurate, high‐throughput way to resequence genomes of interest by comparison to a reference genome. Mate‐paired in vitro shotgun genomic libraries are produced and clonally amplified on microbeads by emulsion PCR. These serve as templates for sequencing by fluorescent nonamer ligation reactions on
Gregory J, Porreca +2 more
openaire +2 more sources
Science, 1999
The News Focus article by Eliot Marshall titled “A high-stakes gamble on genome sequencing” (18 June, p. [1906][1]) states that the PE Biosystems model 3700 DNA sequencer was developed by industry. While PE Biosystems invested significant resources in their outstanding engineering work for the instrument, the original prototypes were developed by H ...
openaire +2 more sources
The News Focus article by Eliot Marshall titled “A high-stakes gamble on genome sequencing” (18 June, p. [1906][1]) states that the PE Biosystems model 3700 DNA sequencer was developed by industry. While PE Biosystems invested significant resources in their outstanding engineering work for the instrument, the original prototypes were developed by H ...
openaire +2 more sources
Nature Methods, 2021
Loop-seq is a high-throughput sequencing assay that measures DNA looping and can help explain how DNA bendability contributes to nucleosome organization.
openaire +2 more sources
Loop-seq is a high-throughput sequencing assay that measures DNA looping and can help explain how DNA bendability contributes to nucleosome organization.
openaire +2 more sources