Results 221 to 230 of about 1,646,380 (263)
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Science, 1993
This article describes the emerging technologies for rapid sequencing of DNA. Technologies discussed include the use of ultrathin gels, replaceable or reusable gels, automated systems, new types of primers to replace the current shotgun method of DNA sequencing, mass spectrometric direct sequencing technologies, and sequencing by hybridization.
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This article describes the emerging technologies for rapid sequencing of DNA. Technologies discussed include the use of ultrathin gels, replaceable or reusable gels, automated systems, new types of primers to replace the current shotgun method of DNA sequencing, mass spectrometric direct sequencing technologies, and sequencing by hybridization.
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Analytical Biochemistry, 1988
A modification of Lin's systematic DNA sequencing strategy is described. A method based on the religation of compatible cohesive ends generated by Sau3AI and BamHI was developed. The original procedure has been simplified and the yield of transfectant has been greatly improved.
Y M, Lee, S C, Lee
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A modification of Lin's systematic DNA sequencing strategy is described. A method based on the religation of compatible cohesive ends generated by Sau3AI and BamHI was developed. The original procedure has been simplified and the yield of transfectant has been greatly improved.
Y M, Lee, S C, Lee
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On enumerating the DNA sequences
Proceedings of the ACM Conference on Bioinformatics, Computational Biology and Biomedicine, 2012DNA sequences are denoted mostly in raw text format, where 2-bit compressed representations are preferred either to gain space or perform some processing fast via computers intrinsic bitwise operations, e.g., search applications. Studies aiming to compress DNA sequences have deployed various encoding schemes such as Lempel--Ziv type factorizations ...
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2010
Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Yvonne, Wallis, Natalie, Morrell
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Fluorescent cycle sequencing of PCR products is a multistage process and several methodologies are available to perform each stage. This chapter will describe the more commonly utilised dye-terminator cycle sequencing approach using BigDye® terminator chemistry (Applied Biosystems) ready for analysis on a 3730 DNA genetic analyzer.
Yvonne, Wallis, Natalie, Morrell
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Proceedings of the Paris C. Kanellakis memorial workshop on Principles of computing & knowledge: Paris C. Kanellakis memorial workshop on the occasion of his 50th birthday, 2003
Paired-read shotgun sequencing of a genome consists of randomly sampling segments of a fixed length, say 10,000 base pairs (10Kbp), and directly determining a "read" of 500 to 700bp at the two ends of each segment with an automated DNA sequencing instrument.
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Paired-read shotgun sequencing of a genome consists of randomly sampling segments of a fixed length, say 10,000 base pairs (10Kbp), and directly determining a "read" of 500 to 700bp at the two ends of each segment with an automated DNA sequencing instrument.
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[Proceedings] DCC `93: Data Compression Conference, 2002
The authors propose a lossless algorithm based on regularities, such as the presence of palindromes, in the DNA. The results obtained, although not satisfactory, are far beyond classical algorithms. >
Stéphane Grumbach, Fariza Tahi
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The authors propose a lossless algorithm based on regularities, such as the presence of palindromes, in the DNA. The results obtained, although not satisfactory, are far beyond classical algorithms. >
Stéphane Grumbach, Fariza Tahi
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Analytical Chemistry, 1995
Faster methods than are currently available will be needed to sequence the billions of base pairs of DNA in the human genome in a reasonable number of years and without exhausting available funds. A new sequencing technology in which DNA binds or hybridizes' to an array of oligonucleotides on a silicon chip appears to be promising as a high-throughput ...
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Faster methods than are currently available will be needed to sequence the billions of base pairs of DNA in the human genome in a reasonable number of years and without exhausting available funds. A new sequencing technology in which DNA binds or hybridizes' to an array of oligonucleotides on a silicon chip appears to be promising as a high-throughput ...
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Statistical Methods in Medical Research, 1993
Recent developments in the statistical analysis of DNA sequences are reviewed. The pace with which sequence data are being generated and analysed has increased with the growth of the human genome project. Two areas of activity are emphasized: attention to error rates in recorded sequences, and heterogeneity in structure of sequences.
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Recent developments in the statistical analysis of DNA sequences are reviewed. The pace with which sequence data are being generated and analysed has increased with the growth of the human genome project. Two areas of activity are emphasized: attention to error rates in recorded sequences, and heterogeneity in structure of sequences.
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2003
The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
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The polymerase chain reaction (PCR), first published by Mullis and Faloona in 1987 (1), has become an invaluable molecular biology tool in both research and routine applications. The combination of PCR with the chain-termination sequencing technique developed by Sanger et al.
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