Results 11 to 20 of about 3,135 (178)
Supercoiling DNA optically [PDF]
Proceedings of the National Academy of Sciences, 2019 AbstractCellular DNA is regularly subject to torsional stress during genomic processes, such as transcription and replication, resulting in a range of supercoiled DNA structures.1,2,3 For this reason, methods to prepare and study supercoiled DNA at the single-molecule level are widely used, including magnetic,4,5,6 angular-optical,7,8,9 micro-pipette ...
King, Graeme A. +3 more
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Mechanistic basis for relaxation of DNA supercoils by human topoisomerase IIIα-RMI1-RMI2. [PDF]
Proc Natl Acad Sci U S ASpakman D +6 more
europepmc +3 more sources
Interaction of the Escherichia coli HU Protein with Various Topological Forms of DNA
Biomolecules, 2021 E. coli histone-like protein HU has been shown to interact with different topological forms of DNA. Using radiolabeled HU, we examine the effects of DNA supercoiling on HU–DNA interactions.
Li Huang, Zhenfeng Zhang, Roger McMacken
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Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2022 Two new copper(II) complexes of bidentate arylhydrazone ligands have prepared and studied. Elemental analysis, SEM-EDS, thermal gravimetric analysis (TGA), powder X-ray diffraction (XRD), and a number of spectroscopic techniques were used to determine ...
Cansu Topkaya
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Supercoiling DNA Locates Mismatches [PDF]
Physical Review Letters, 2017 We present a method of detecting sequence defects by supercoiling DNA with magnetic tweezers. The method is sensitive to a single mismatched base pair in a DNA sequence of several thousand base pairs. We systematically compare DNA molecules with 0 to 16 adjacent mismatches at 1Mmonovalent salt and 3.5 pN force and show that, under these conditions, a ...
Dittmore, Andrew +4 more
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DNA knots and DNA supercoiling [PDF]
Cell Cycle, 2011 Keywords: DNA topoisomerases; DNA topology; DNA structure Reference EPFL-ARTICLE-167094doi:10.4161/cc.10.9.15293View record in Web of Science Record created on 2011-06-24, modified on 2017-05 ...
Guillaume, Witz +2 more
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Mechanics of the IL2RA gene activation revealed by modeling and atomic force microscopy. [PDF]
PLoS ONE, 2011 Transcription implies recruitment of RNA polymerase II and transcription factors (TFs) by DNA melting near transcription start site (TSS). Combining atomic force microscopy and computer modeling, we investigate the structural and dynamical properties of ...
Pascale Milani +8 more
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Sen1 and Rrm3 ensure permissive topological conditions for replication termination
Cell Reports, 2023 Summary: Replication forks terminate at TERs and telomeres. Forks that converge or encounter transcription generate topological stress. Combining genetics, genomics, and transmission electron microscopy, we find that Rrm3hPif1 and Sen1hSenataxin ...
Ramveer Choudhary +6 more
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DNA supercoiling inhibits DNA knotting [PDF]
Nucleic Acids Research, 2008 Despite the fact that in living cells DNA molecules are long and highly crowded, they are rarely knotted. DNA knotting interferes with the normal functioning of the DNA and, therefore, molecular mechanisms evolved that maintain the knotting and catenation level below that which would be achieved if the DNA segments could pass randomly through each ...
Burnier Y, Dorier J, Stasiak A
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Preferential binding of hot spot mutant p53 proteins to supercoiled DNA in vitro and in cells. [PDF]
PLoS ONE, 2013 Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53 to DNA is assumed to be involved in mutp53-mediated repression or activation of several mutp53 target genes.
Marie Brázdová +11 more
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