Results 41 to 50 of about 3,135 (178)

Biomanufacturing Potential of Streamlined Cells

Biotechnology and Bioengineering, Volume 122, Issue 12, Page 3309-3318, December 2025.
ABSTRACT A series of Escherichia coli streamlined strains was developed by removing the expression of genes encoding extracellular structures and unessential enzymes. The streamlined strains exhibited improved metabolic performance, including lower overflow metabolism and ATP maintenance coefficient, as well as a higher growth rate, compared to their ...
Alvaro R. Lara, Marie B. Andersen, Alexander A. V. Madsen, Kathrine Gravlund Fønss, Marta Irla, Hilal Taymaz‐Nikerel, Luz María Martínez, Max Daniel Dicke, Marcel Mann, Jørgen B. Magnus, Guillermo Gosset   +10 more
wiley   +1 more source

The Concise Guide to PHARMACOLOGY 2025/26: Introduction and Other Protein Targets

British Journal of Pharmacology, Volume 182, Issue S1, Page S1-S23, December 2025.
The Concise Guide to Pharmacology 2025/26 marks the seventh edition in this series of biennial publications in the British Journal of Pharmacology. Presented in landscape format, the guide provides a comparative overview of the pharmacology of drug target families. The concise nature of the Concise Guide refers to the style of presentation, being clear,
Stephen P. H. Alexander, Alasdair J. Gibb, Eamonn Kelly, Alistair A. Mathie, Chloe J. Peach, Emma L. Veale, John A. Cidlowski, Anthony P. Davenport, Doriano Fabbro, Michael Spedding, Jörg Striessnig, Jane F. Armstrong, O. Peter Buneman, Elena Faccenda, Simon D. Harding, Christopher Southan, Jamie A. Davies, Katelin E. Ahlers‐Dannen, Mohammed Alqinyah, Thiruma V. Arumugam, Christopher Bodle, Josephine Buo Dagner, Bandana Chakravarti, Shreoshi P. Choudhuri, Kirk M. Druey, Rory A. Fisher, Kyle J. Gerber, John R. Hepler, Shelley B. Hooks, Havish S. Kantheti, Behirda Karaj, Somayeh Layeghi‐Ghalehsoukhteh, Jae‐Kyung Lee, Zili Luo, Kirill Martemyanov, Luke D. Mascarenhas, Harrison McNabb, Carolina Montañez‐Miranda, Osita Ogujiofor, Hoa Phan, David L. Roman, Vincent Shaw, Benita Sjogren, Christopher Sobey, Mackenzie M. Spicer, Katherine E. Squires, Laurie Sutton, Menbere Wendimu, Thomas Wilkie, Keqiang Xie, Qian Zhang, Yalda Zolghadri   +51 more
wiley   +1 more source

Comprehensive review of DNA gyrase as enzymatic target for drug discovery and development

European Journal of Medicinal Chemistry Reports
DNA gyrase is a member of the DNA topoisomerase protein family that catalyzes the conversion of different topological forms of DNA into one another. It is the sole enzyme that causes DNA to negatively supercoil. The enzyme is tetrameric with two GyrA (“A'
K. Rajakumari, K. Aravind, M. Balamugundhan, Manjunathan Jagadeesan, Ambiga Somasundaram, Parthiban Brindha Devi, Pasiyappazham Ramasamy   +6 more
doaj   +1 more source

Bee‐Derived Antioxidants as a Protective Strategy Against Doxorubicin‐Induced Ovarian Damage

Chemistry &Biodiversity, Volume 22, Issue 11, November 2025.
Graphical abstract of Bee‐Derived Antioxidants as a Protective Strategy Against Doxorubicin‐Induced Ovarian Damage ABSTRACT Antineoplastic agents can induce tissue damage through oxidative stress mechanisms. Doxorubicin, a widely used chemotherapeutic agent, has been shown to cause permanent damage to reproductive tissues.
Meltem Arıkan Malkoc, Serap Özer Yaman, Şafak Ersöz, Sevgi Kolaylı   +3 more
wiley   +1 more source

Supercoiling in DNA and chromatin

Current Opinion in Genetics & Development, 2014
Supercoiling is a fundamental property of DNA and chromatin. It is modulated by polymerase and topoisomerase activities and, through regulated constraint, by DNA/chromatin binding proteins. As a non-covalent and elusive topological modification, supercoiling has proved intractable to research despite being a crucial regulator of nuclear structure and ...
Gilbert, N., Allan, J.
openaire   +3 more sources

The Lrs14‐Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid‐Organizing Protein

MicrobiologyOpen, Volume 14, Issue 5, October 2025.
Nucleoid organization in Crenarchaeota involves diverse small DNA‐binding proteins. The Lrs14‐type protein AbfR1 from Metallosphaera sedula binds nonsequence specifically across the genome and induces DNA condensation. These findings suggest a structural role for AbfR1Ms in chromatin architecture, functionally resembling bacterial nucleoid‐associated ...
Veerke De Kock, Ronnie Willaert, Yannick Gansemans, Filip Van Nieuwerburgh, Rani Baes, Eveline Peeters   +5 more
wiley   +1 more source

Effects of Static and Low‐Frequency Magnetic Fields on Gene Expression

Journal of Magnetic Resonance Imaging, Volume 62, Issue 2, Page 317-334, August 2025.
Substantial research over the past two decades has established that magnetic fields affect fundamental cellular processes, including gene expression. However, since biological cells and subcellular components exhibit diamagnetic behavior and are therefore subjected to very small magnetic forces that cannot directly compete with the viscoelastic and ...
Vitalii Zablotskii, Oksana Gorobets, Svitlana Gorobets, Tatyana Polyakova   +3 more
wiley   +1 more source

The Life of a Kidney Podocyte

Acta Physiologica, Volume 241, Issue 8, August 2025.
ABSTRACT Aim Podocytes, highly specialized epithelial cells located in the glomerulus of the kidney, are essential to the filtration barrier that ensures separation of blood and urine. These cells exhibit a unique architecture, characterized by an intricate network of foot processes interconnected by slit diaphragms, which serve as a critical selective
Desiree Loreth, Wiebke Sachs, Catherine Meyer‐Schwesinger   +2 more
wiley   +1 more source

Ratiometric nonfluorescent CRISPR assay utilizing Cas12a-induced plasmid supercoil relaxation

Communications Chemistry
Most CRISPR-based biosensors rely on labeled reporter molecules and expensive equipment for signal readout. A recent approach quantifies analyte concentration by sizing λ DNA reporters via gel electrophoresis, providing a simple solution for label-free ...
Noor Mohammad, Logan Talton, Selen Dalgan, Zach Hetzler, Anastasiia Steksova, Qingshan Wei   +5 more
doaj   +1 more source

DNA Supercoiling Measurement in Bacteria [PDF]

, 2017
DNA supercoiling plays critical roles in several essential DNA metabolic pathways, such as replication, transcription and recombination. Typically plasmid DNA molecules are used to measure DNA supercoiling status inside bacterial cells. In this chapter, we describe how to isolate plasmid DNA molecules from E.
Yingting, Liu, Zhi-Chun, Hua, Fenfei, Leng   +2 more
openaire   +2 more sources