Results 51 to 60 of about 3,135 (178)

Programmable Protein‐DNA Composite Nanostructures: from Nanostructure Construction to Protein‐Induced Micro‐Scale Material Self‐Assembly and Functionalization

Small, Volume 21, Issue 30, July 29, 2025.
This study presents a modular platform that integrates engineered proteins with DNA nanostructures to create dynamic, multifunctional materials. By combining orthogonal protein‐protein and protein‐DNA conjugation strategies, the authors demonstrate controlled self‐assembly into nanofibers and nanorods, along with reversible enzyme activation.
Weijun Zhou, Žiga Strmšek, Jaka Snoj, Miha Škarabot, Roman Jerala   +4 more
wiley   +1 more source

Human Smc5/6 recognises transcription-generated positive DNA supercoils [PDF]

, 2023
Aurélie Diman, Gaël Panis, Cédric Castrogiovanni, Julien Prados, Bastien Baechler, Michel Strubin   +5 more
openalex   +1 more source

Structural diversity of supercoiled DNA [PDF]

Nature Communications, 2015
Abstract By regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism. Despite its importance, however, much about supercoiled DNA (positively supercoiled DNA, in particular) remains unknown. Here we use electron cryo-tomography together with biochemical analyses to investigate structures of individual ...
Rossitza N, Irobalieva, Jonathan M, Fogg, Daniel J, Catanese, Daniel J, Catanese, Thana, Sutthibutpong, Muyuan, Chen, Anna K, Barker, Steven J, Ludtke, Sarah A, Harris, Michael F, Schmid, Wah, Chiu, Lynn, Zechiedrich   +11 more
openaire   +3 more sources

Unraveling the Role of Topoisomerase 3β (TOP3B) in mRNA Translation and Human Disease

WIREs RNA, Volume 16, Issue 4, July/August 2025.
The human genome encodes six topoisomerases—TOP1, TOP1mt, TOP2A, TOP2B, TOP3A, and TOP3B. The subcellular localization of each is shown. TOP3B, which harbors a bona fide RNA‐binding domain, predominantly localizes to the cytosol and is reported to function in mRNA translation and stability.
Julia E. Warrick, Michael G. Kearse
wiley   +1 more source

Rapid, DNA-induced interface swapping by DNA gyrase

eLife
DNA gyrase, a ubiquitous bacterial enzyme, is a type IIA topoisomerase formed by heterotetramerisation of 2 GyrA subunits and 2 GyrB subunits, to form the active complex.
Thomas RM Germe, Natassja G Bush, Victoria M Baskerville, Dominik Saman, Justin LP Benesch, Anthony Maxwell   +5 more
doaj   +1 more source

The Redox Activity of Protein Disulphide Isomerase Functions in Non‐Homologous End‐Joining Repair to Prevent DNA Damage

Aging Cell, Volume 24, Issue 7, July 2025.
Schematic diagram illustrating the protective role of protein disulphide isomerase (PDI) against DNA damage via non‐homologous end‐joining (NHEJ), which repairs double stranded DNA breaks (DSBs). Induction of DNA damage results in the formation of γH2AX and p53‐binding protein 1 foci during NHEJ repair of DSBs.
Sina Shadfar, Fabiha Farzana, Sayanthooran Saravanabavan, Ashley M. Rozario, Marta Vidal, Cyril Jones Jagaraj, Sonam Parakh, Esmeralda Paric, Kristy C. Yuan, Mariana Brocardo, Donna R. Whelan, Angela S. Laird, Julie D. Atkin   +12 more
wiley   +1 more source

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, Volume 599, Issue 12, Page 1749-1759, June 2025.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Phenolic profile, bioactivity and cytotoxicity of plant extracts from thyme, ginger, garlic, ground roasted coffee and coffee silverskin

International Journal of Food Properties
We evaluated selected biological properties of extracts from thyme (THY) (Thymus vulgaris L.), ginger (GNG) (Zingiber officinale Roscoe), garlic (GLC) (Allium sativum L.), ground roasted coffee (GRC), and coffee silverskin (CS) (Coffea sp. L.).
Fabíola de Lima Pena, Mariana Correa de Souza, Vitor Lacerda Sanches, Juliane Viganó, Mariana Camargo Silva Mancini, Thaís Carvalho Brito-Oliveira, Bárbara Geciana Tomaz dos Santos, Guilherme Henrique Aguiar de Queiroz, Leticia Tamborlin, Fernando Moreira Simabuco, Augusto Ducati Luchessi, Maurício Ariel Rostagno, Adriane Elisabete Costa Antunes   +12 more
doaj   +1 more source

DNA supercoiling in gyrase mutants [PDF]

Journal of Bacteriology, 1984
Nucleoids isolated from Escherichia coli strains carrying temperature-sensitive gyrA or gyrB mutations were examined by sedimentation in ethidium bromide-containing sucrose density gradients. A shift to restrictive temperature resulted in nucleoid DNA relaxation in all of the mutant strains.
T R, Steck, G J, Pruss, S H, Manes, L, Burg, K, Drlica   +4 more
openaire   +2 more sources

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria

FEBS Open Bio, Volume 15, Issue 6, Page 949-962, June 2025.
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama   +4 more
wiley   +1 more source