Results 11 to 20 of about 113,306 (267)

A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome [PDF]

, 2019
DNA topoisomerases are required to resolve DNA topological stress. Despite this essential role, abortive topoisomerase activity generates aberrant protein-linked DNA breaks, jeopardising genome stability.
Allison, Rachal M, Cooper, Tim J, Gittens, William H, Johnson, Dominic J, Neale, Matthew J, Thomas, Holly   +5 more
core   +1 more source

Determination of T Cell Responses in Thai Systemic Sclerosis Patients

Journal of Immunology Research, 2022
Objectives. This study is aimed at determining the role of T cells by assessing the numbers of IFN-γ- and IL-2-secreting T cells following stimulation with peptides derived from DNA topoisomerase-I protein in Thai SSc patients. Methods.
Oranit Likhit, Worawit Louthrenoo, Sa-nga Pattanakitsakul, Aroonroong Suttitheptumrong, Supot Hannongbua, Thanyada Rungrotmongkol, Hiroshi Noguchi, Fujio Takeuchi, Kobporn Boonnak   +8 more
doaj   +1 more source

Gel-Free Tools for Quick and Simple Screening of Anti-Topoisomerase 1 Compounds

Pharmaceuticals, 2023
With the increasing need for effective compounds against cancer or pathogen-borne diseases, the development of new tools to investigate the enzymatic activity of biomarkers is necessary.
Josephine Geertsen Keller, Kamilla Vandsø Petersen, Karol Mizielinski, Celine Thiesen, Lotte Bjergbæk, Rosa M. Reguera, Yolanda Pérez-Pertejo, Rafael Balaña-Fouce, Angela Trejo, Carme Masdeu, Concepcion Alonso, Birgitta R. Knudsen, Cinzia Tesauro   +12 more
doaj   +1 more source

Distinct requirements for the Rad32(Mre¹¹) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA [PDF]

, 2009
For a cancer cell to resist treatment with drugs that trap topoisomerases covalently on the DNA, the topoisomerase must be removed. In this study, we provide evidence that the Schizosaccharomyces pombe Rad32Mre11 nuclease activity is involved in the ...
Akamatsu, Antony M. Carr, Baldwin, Champoux, Connelly, Connelly, Edgar Hartsuiker, El-Khamisy, Farah, Gutz, Hartsuiker, Hopfner, Hopfner, Keeney, Keeney, Krogh, Krogh, Lengsfeld, Limbo, Liu, Malik, Matthew J. Neale, McKee, Miyoshi, Moreau, Neale, Nitiss, Osheroff, Porter, Pouliot, Prinz, Sartori, Shaw, Stracker, Subramanian, Takashima, Tomita, Utsugi, Young   +38 more
core   +2 more sources

Why Should DNA Topoisomerase I Have a Scaffold Activity?

Biology, 2021
Since the early 1990s, in vitro studies have demonstrated that DNA topoisomerase I promotes RNA polymerase II transcription, acting as a cofactor, regardless of its catalytic activity.
Francesca Di Felice, Giorgio Camilloni
doaj   +1 more source

TDP2 promotes repair of topoisomerase I-mediated DNA damage in the absence of TDP1 [PDF]

, 2012
The abortive activity of topoisomerases can result in clastogenic and/or lethal DNA damage in which the topoisomerase is covalently linked to the 3'- or 5'-terminus of a DNA strand break. This type of DNA damage is implicated in chromosome translocations
Abhishek Sharma, Barthelmes, Danny Huylebroeck, Davies, Davies, El-Khamisy, El-Khamisy, El-Khamisy, El-Khamisy, Esguerra, Giovanella, Hirano, Hudson, Interthal, Interthal, Junko Murai, Katyal, Keith W. Caldecott, Ledesma, Liesbeth Vermeire, Lieve Umans, Limei Ju, Murai, Nitiss, Pommier, Pommier, Pype, Sherif F. El-Khamisy, Shunichi Takeda, Takashima, Varady, Vilotti, Yang, Yves Pommier, Zeng, Zhihong Zeng, Zhou   +36 more
core   +1 more source

Numerical analysis of etoposide induced DNA breaks. [PDF]

PLoS ONE, 2009
BACKGROUND:Etoposide is a cancer drug that induces strand breaks in cellular DNA by inhibiting topoisomerase II (topoII) religation of cleaved DNA molecules.
Aida Muslimović, Susanne Nyström, Yue Gao, Ola Hammarsten   +3 more
doaj   +1 more source

Simple and Fast DNA Based Sensor System for Screening of Small-Molecule Compounds Targeting Eukaryotic Topoisomerase 1

Pharmaceutics, 2021
Background: Eukaryotic topoisomerase 1 is a potential target of anti-parasitic and anti-cancer drugs. Parasites require topoisomerase 1 activity for survival and, consequently, compounds that inhibit topoisomerase 1 activity may be of interest.
Kamilla Vandsø Petersen, Asier Selas, Kirstine Mejlstrup Hymøller, Karol Mizielinski, Maria Thorsager, Magnus Stougaard, Concepcion Alonso, Francisco Palacios, Yolanda Pérez-Pertejo, Rosa M. Reguera, Rafael Balaña-Fouce, Birgitta R. Knudsen, Cinzia Tesauro   +12 more
doaj   +1 more source

Fork rotation and DNA precatenation are restricted during DNA replication to prevent chromosomal instability [PDF]

, 2015
Faithful genome duplication and inheritance require the complete resolution of all intertwines within the parental DNA duplex. This is achieved by topoisomerase action ahead of the replication fork or by fork rotation and subsequent resolution of the DNA
Anna L. Chambers, Hiasa, Jessica A. Downs, Jonathan Baxter, Sahar Mansoubi, Stephanie A. Schalbetter   +5 more
core   +3 more sources

Role of Topoisomerase IIβ in DNA Damage Response following IR and Etoposide

Journal of Nucleic Acids, 2010
The role of topoisomerase IIβ was investigated in cell lines exposed to two DNA damaging agents, ionising radiation (IR) or etoposide, a drug which acts on topoisomerase II. The appearance and resolution of γH2AX foci in murine embryonic fibroblast cell
Nicola J. Sunter, Ian G. Cowell, Elaine Willmore, Gary P. Watters, Caroline A. Austin   +4 more
doaj   +1 more source