Results 1 to 10 of about 9,967 (240)

Trapping of the transport-segment DNA by the ATPase domains of a type II topoisomerase [PDF]

Nature Communications, 2018
Abstract Type II topoisomerases alter DNA topology to control DNA supercoiling and chromosome segregation and are targets of clinically important anti-infective and anticancer therapeutics. They act as ATP-operated clamps to trap a DNA helix and transport it through a transient break in a second DNA.
Ivan Laponogov, Xiao-Su Pan, Dennis A. Veselkov, Galyna B. Skamrova, Trishant R. Umrekar, L. Mark Fisher, Mark R. Sanderson   +6 more
openaire   +6 more sources

Topoisomerase IIβ targets DNA crossovers formed between distant homologous sites to induce chromatin opening

Scientific Reports, 2020
Type II DNA topoisomerases (topo II) flip the spatial positions of two DNA duplexes, called G- and T- segments, by a cleavage-passage-resealing mechanism. In living cells, these DNA segments can be derived from distant sites on the same chromosome.
Mary Miyaji, Ryohei Furuta, Osamu Hosoya, Kuniaki Sano, Norikazu Hara, Ryozo Kuwano, Jiyoung Kang, Masaru Tateno, Kimiko M. Tsutsui, Ken Tsutsui   +9 more
doaj   +1 more source

Crystal Structure of an Intact Type II DNA Topoisomerase: Insights into DNA Transfer Mechanisms [PDF]

Structure, 2008
DNA topoisomerases resolve DNA topological problems created during transcription, replication, and recombination. These ubiquitous enzymes are essential for cell viability and are highly potent targets for the development of antibacterial and antitumoral drugs.
Graille, Marc, Cladière, Lionel, Durand, Dominique, Lecointe, François, Gadelle, Danièle, Quevillon-Cheruel, Sophie, Vachette, Patrice, Forterre, Patrick, van Tilbeurgh, Herman   +8 more
openaire   +2 more sources

The antimicrobial activity of exogeno us anionic phospholipids against Mycobacterium tuberculosis and Escherichia coli

Тонкие химические технологии, 2016
The effect of anionic phospholipids, namely, cardiolipin, phosphatidylglycerol and phosphatidic acid, on the growth of gram-negative bacteria E. coli BL21(DE3), as well as gram-positive bacteria M. tuberculosis H37Rv was investigated in this study.
Yu. L. Mikulovich, G. M. Sorokoumova, А. А. Selishcheva, V. I. Shvets   +3 more
doaj   +1 more source

Type II DNA Topoisomerases Cause Spontaneous Double-Strand Breaks in Genomic DNA [PDF]

Genes, 2019
Type II DNA topoisomerase enzymes (TOP2) catalyze topological changes by strand passage reactions. They involve passing one intact double stranded DNA duplex through a transient enzyme-bridged break in another (gated helix) followed by ligation of the break by TOP2.
Suguru Morimoto, Masataka Tsuda, Heeyoun Bunch, Hiroyuki Sasanuma, Caroline Austin, Shunichi Takeda   +5 more
openaire   +2 more sources

Synthesis and In Vitro Cytotoxic Activity of N-2-(2-Furyl)-2-(chlorobenzyloxyimino)Ethyl Ciprofloxacin Derivatives

E-Journal of Chemistry, 2011
Quinolone are a class of potent broad-spectrum antibacterial drugs that target the bacterial type II DNA topoisomerases (DNA gyrase) and topoisomerase IV.
Eskandar Alipour, Negar Mohammad Hosseini, Fatemeh Panah, Sussan K. Ardestani, Maliheh Safavi, Abbas Shafiee, Alireza Foroumadi   +6 more
doaj   +1 more source

Breakage-reunion domain of Streptococcus pneumoniae topoisomerase IV: crystal structure of a gram-positive quinolone target.

PLoS ONE, 2007
The 2.7 A crystal structure of the 55-kDa N-terminal breakage-reunion domain of topoisomerase (topo) IV subunit A (ParC) from Streptococcus pneumoniae, the first for the quinolone targets from a gram-positive bacterium, has been solved and reveals a ...
Ivan Laponogov, Dennis A Veselkov, Maninder K Sohi, Xiao-Su Pan, Aniruddha Achari, Cheng Yang, Joseph D Ferrara, L Mark Fisher, Mark R Sanderson   +8 more
doaj   +1 more source

The African Swine Fever Virus (ASFV) Topoisomerase II as a Target for Viral Prevention and Control

Vaccines, 2020
African swine fever (ASF) is, once more, spreading throughout the world. After its recent reintroduction in Georgia, it quickly reached many neighboring countries in Eastern Europe.
João Coelho, Alexandre Leitão
doaj   +1 more source

Amsacrine Derivatives Selectively Inhibit Mycobacterial Topoisomerase I (TopA), Impair M. smegmatis Growth and Disturb Chromosome Replication

Frontiers in Microbiology, 2018
Amsacrine, which inhibits eukaryotic type II topoisomerase via DNA intercalation and stabilization of the cleavable topoisomerase-DNA complex, promotes DNA damage and eventually cell death.
Marcin J. Szafran, Marta Kołodziej, Patrycja Skut, Brahmam Medapi, Agnieszka Domagała, Damian Trojanowski, Jolanta Zakrzewska-Czerwińska, Jolanta Zakrzewska-Czerwińska, Dharmarajan Sriram, Dagmara Jakimowicz, Dagmara Jakimowicz   +10 more
doaj   +1 more source

Over-expression of Topoisomerase II Enhances Salt Stress Tolerance in Tobacco

Frontiers in Plant Science, 2016
Topoisomerases are unique enzymes having an ability to remove or add DNA supercoils and untangle the snarled DNA. They can cut, shuffle and religate DNA strands and remove the torsional stress during DNA replication, transcription or recombination events.
Riffat John, Uma Ganeshan, Badri Nath Singh, Tanushri Kaul, Malireddy K Reddy, Sudhir Kumar Sopory, Manchikatla Venkat Rajam   +6 more
doaj   +1 more source