Results 11 to 20 of about 9,418 (226)
DNA-Topology Simplification by Topoisomerases
Molecules, 2021 The topological properties of DNA molecules, supercoiling, knotting, and catenation, are intimately connected with essential biological processes, such as gene expression, replication, recombination, and chromosome segregation. Non-trivial DNA topologies
Andreas Hanke +2 more
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Conversion of DNA gyrase into a conventional type II topoisomerase [PDF]
Proceedings of the National Academy of Sciences, 1996 DNA gyrase is unique among topoisomerases in its ability to introduce negative supercoils into closed-circular DNA. We have demonstrated that deletion of the C-terminal DNA-binding domain of the A subunit of gyrase gives rise to an enzyme that cannot supercoil DNA but relaxes DNA in an ATP-dependent manner.
Kampranis, S C, Maxwell, A
openaire +2 more sources
SUMO: A Swiss Army Knife for Eukaryotic Topoisomerases
Frontiers in Molecular Biosciences, 2022 Topoisomerases play crucial roles in DNA metabolism that include replication, transcription, recombination, and chromatin structure by manipulating DNA structures arising in double-stranded DNA.
Yilun Sun, John L. Nitiss, Yves Pommier
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Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China [PDF]
Archives of Biological Sciences, 2021 Type II topoisomerases, including DNA gyrase (GyrA) and topoisomerase IV (ParC), contribute to fluoroquinolone resistance in Enterococcus spp. This study investigated the mutational status of the quinolone resistance-determining regions (QRDRs) of GyrA ...
Su Rina +4 more
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Mechanism of topology simplification by type II DNA topoisomerases [PDF]
Proceedings of the National Academy of Sciences, 2001 Type II DNA topoisomerases actively reduce the fractions of knotted and catenated circular DNA below thermodynamic equilibrium values. To explain this surprising finding, we designed a model in which topoisomerases introduce a sharp bend in DNA. Because the enzymes have a specific orientation relative to the bend, they act like Maxwell's demon ...
A V, Vologodskii +6 more
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Transcription facilitated genome-wide recruitment of topoisomerase I and DNA gyrase. [PDF]
PLoS Genetics, 2017 Movement of the transcription machinery along a template alters DNA topology resulting in the accumulation of supercoils in DNA. The positive supercoils generated ahead of transcribing RNA polymerase (RNAP) and the negative supercoils accumulating behind
Wareed Ahmed +5 more
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Inhibition of Zn(II) binding type IA topoisomerases by organomercury compounds and Hg(II). [PDF]
PLoS ONE, 2015 Type IA topoisomerase activities are essential for resolving DNA topological barriers via an enzyme-mediated transient single strand DNA break. Accumulation of topoisomerase DNA cleavage product can lead to cell death or genomic rearrangement.
Bokun Cheng +4 more
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Electrostatics of DNA–DNA juxtapositions: consequences for type II topoisomerase function [PDF]
Journal of Physics: Condensed Matter, 2006 Type II topoisomerases resolve problematic DNA topologies such as knots, catenanes, and supercoils that arise as a consequence of DNA replication and recombination. Failure to remove problematic DNA topologies prohibits cell division and can result in cell death or genetic mutation. Such catastrophic consequences make topoisomerases an effective target
Graham L, Randall +3 more
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Antibiotics, 2023 Novel bacterial topoisomerase inhibitors (NBTIs) are a new class of antibacterial agents that target bacterial type II topoisomerases (DNA gyrase and topoisomerase IV). Our recently disclosed crystal structure of an NBTI ligand in complex with DNA gyrase
Maja Kokot +5 more
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DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia. [PDF]
PLoS Neglected Tropical Diseases, 2013 The protozoan Giardia lamblia differentiates into infectious cysts within the human intestinal tract for disease transmission. Expression of the cyst wall protein (cwp) genes increases with similar kinetics during encystation.
Bo-Chi Lin +7 more
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