Results 1 to 10 of about 9,767 (189)

Characterisation of size variants of type I DNA topoisomerase isolated from calf thymus [PDF]

European Journal of Biochemistry, 1984
Calf thymus DNA topoisomerase I, which belongs to the eukaryotic type I topoisomerases, is in a typical preparation purified as a set of five major polypeptides with Mr between 70000 and 100000. At least four of these proteins have binding affinity for DNA as was shown by incubating them with radioactive single‐stranded DNA after separation in ...
Schmitt, B., Schuh-Buhre, U., Vosberg, H.   +2 more
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In vitro investigating of anticancer activity of new 7-MEOTA-tacrine heterodimers

Journal of Enzyme Inhibition and Medicinal Chemistry, 2019
A combination of biochemical, biophysical and biological techniques was used to study calf thymus DNA interaction with newly synthesized 7-MEOTA-tacrine thiourea 12–17 and urea heterodimers 18–22, and to measure interference with type I and II ...
Jana Janockova, Jan Korabecny, Jana Plsikova, Katerina Babkova, Eva Konkolova, Dana Kucerova, Jana Vargova, Jan Koval, Rastislav Jendzelovsky, Peter Fedorocko, Jana Kasparkova, Viktor Brabec, Jan Rosocha, Ondrej Soukup, Slavka Hamulakova, Kamil Kuca, Maria Kozurkova   +16 more
doaj   +1 more source

Role of Supercoiling and Topoisomerases in DNA Knotting

DNA
DNA knots are deleterious for living cells if not removed. Several theoretical and simulation approaches address the question of how topoisomerases select the intermolecular passages that preferentially lead to unknotting rather than to the knotting of ...
Jorge Cebrián, María-Luisa Martínez-Robles, Victor Martínez, Pablo Hernández, Dora B. Krimer, Jorge B. Schvartzman, María-José Fernández-Nestosa   +6 more
doaj   +1 more source

Lucanthone and its derivative hycanthone inhibit apurinic endonuclease-1 (APE1) by direct protein binding.

PLoS ONE, 2011
Lucanthone and hycanthone are thioxanthenone DNA intercalators used in the 1980s as antitumor agents. Lucanthone is in Phase I clinical trial, whereas hycanthone was pulled out of Phase II trials.
Mamta D Naidu, Rakhi Agarwal, Louis A Pena, Luis Cunha, Mihaly Mezei, Min Shen, David M Wilson, Yuan Liu, Zina Sanchez, Pankaj Chaudhary, Samuel H Wilson, Michael J Waring   +11 more
doaj   +1 more source

Genomic and Phenotypic Characterization of Streptomyces marxii sp. nov., Producer of Kinanthraquinone B

Microorganisms
Describing novel microbial species opens access to uncharted biosynthetic gene clusters and their associated secondary metabolites, offering fresh opportunities in the search for new antibiotics urgently needed to combat multidrug resistance.
Mikhail Yu. Dobryakov, Julia A. Buyuklyan, Mikhail V. Biryukov   +2 more
doaj   +1 more source

Crystal Structures, Molecular Docking and In Vitro Investigations of Two 4-Substituted 2-(5,5-dimethyl-3-styrylcyclohex-2-enylidene)malononitrile Derivatives as Potential Topoisomerase II Inhibitors

Crystals
Type II topoisomerases (TOP2s) play a key role in altering the DNA topology by transiently cleaving both strands of a DNA duplex. Therefore, increased TOP2 activity is associated with many cancers.
Martina I. Peeva, Maya G. Georgieva, Aneliya A. Balacheva, Maria Ponticelli, Ivan P. Bogdanov, Tsonko Kolev, Luigi Milella, Hans-Georg Stammler, Nikolay T. Tzvetkov   +8 more
doaj   +1 more source

Inhibitory action of exogenous cardiolipin on the bacterial type i and ii dna topoisomerases in vitro

Тонкие химические технологии, 2012
The influence of empty liposomes composed of cardiolipin (lipid which is a component of the bacterial cytoplasmic membrane and eukaryotic internal mitochondrial membrane) on Escherichia coli topoisomerase I and DNA gyrase (topoisomerase II) in vitro was ...
Yu. L. Mikulovich, G. М. Sorokoumova, А. А. Selishcheva, V. I. Shvets   +3 more
doaj  

Not only the top: Type I topoisomerases function in multiple tissues and organs development in plants

Journal of Advanced Research
Background: DNA topoisomerases (TOPs) are essential components in a diverse range of biological processes including DNA replication, transcription and genome integrity.
Hao Zhang, Lirong Tian, Yuru Ma, Jiahui Xu, Tianyu Bai, Qian Wang, Xigang Liu, Lin Guo   +7 more
doaj   +1 more source

Expression of the type I DNA topoisomerase gene in adenovirus-5 infected human cells

Nucleic Acids Research, 1990
The amount of topoisomerase I specific mRNA increases three- to fivefold during the early phase of infection of HeLa cells with adenovirus-5. The observed increase in specific mRNA is mainly due to an increased rate of transcription of the gene. In human 293 cells, which constitutively express the viral E1A and E1B genes, we determined an elevated ...
H, Romig, A, Richter
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Structural studies of type I DNA topoisomerases [PDF]

Acta Crystallographica Section A Foundations of Crystallography, 1996
A. Mondragón, C. Lima, N. Lue, A. Patera, A. Sharma   +4 more
openaire   +1 more source